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NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="GPR88 was detected in immersion fixed NS0 mouse myeloma cell line transfected with GPR88 (positive staining) and absent in NS0 mouse myeloma cell line (wild type, negative control) using Mouse Anti-Human GPR88 Monoclonal Antibody (Catalog # MAB108281) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />
VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">
VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="GPR88 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Mouse Anti-Human GPR88 Monoclonal Antibody (Catalog # MAB10828) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />