CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="Serpin F2/a2‑Antiplasmin was detected in perfusion fixed frozen sections of mouse intestine using 5 µg/mL Goat Anti-Mouse Serpin F2/a2‑Antiplasmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1239) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1239-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1470-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1470-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90305-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1239-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89394-50ul | |||
![Western Blot: Serpin F2/alpha 2-Antiplasmin Antibody [NB100-96916] - Staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence. Approx 70 kDa band observed in lysates of cell line HepG2 (calculated MW of 54.6 kDa according to NP_000925.2).](http://images.novusbio.com/fullsize/Serpin-F2-alpha-2-Antiplasmin-Antibody-Western-Blot-NB100-96916-img0006.jpg "Western Blot: Serpin F2/alpha 2-Antiplasmin Antibody [NB100-96916] - Staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence. Approx 70 kDa band observed in lysates of cell line HepG2 (calculated MW of 54.6 kDa according to NP_000925.2).")
![Immunocytochemistry/Immunofluorescence: Serpin F2/alpha 2-Antiplasmin Antibody [NB100-96916] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Serpin-F2-alpha-2-Antiplasmin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-96916-img0007.jpg)
![Immunohistochemistry-Paraffin: Serpin F2/alpha 2-Antiplasmin Antibody [NB100-96916] - Staining of paraffin embedded Human Liver. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Serpin-F2-alpha-2-Antiplasmin-Antibody-Immunohistochemistry-NB100-96916-img0003.jpg)
![Flow Cytometry: Serpin F2/alpha 2-Antiplasmin Antibody [NB100-96916] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Serpin-F2-alpha-2-Antiplasmin-Antibody-Flow-Cytometry-NB100-96916-img0008.jpg)
![N/A Serpin F2/alpha 2-Antiplasmin [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Serpin_F2_DY147005_ELISA_2359.jpg "N/A Serpin F2/alpha 2-Antiplasmin [Biotin]")
![ELISA: Human Serpin F2/alpha 2-Antiplasmin ELISA Kit (Colorimetric) [NBP3-08193] - Samples were spiked with high concentrations of Human Serpin F2/alpha 2-Antiplasmin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-08193_human-serpin-f2-alpha-2-antiplasmin-elisa-kit-colorimetric-13220241613063.jpg "ELISA: Human Serpin F2/alpha 2-Antiplasmin ELISA Kit (Colorimetric) [NBP3-08193] - Samples were spiked with high concentrations of Human Serpin F2/alpha 2-Antiplasmin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Serpin F2/alpha 2-Antiplasmin ELISA Kit (Colorimetric) [NBP3-08193] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-08193_human-serpin-f2-alpha-2-antiplasmin-elisa-kit-colorimetric-4320248574334.jpg)
![Western Blot: Serpin F2/alpha 2-Antiplasmin Antibody (001) [NBP2-89394] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:56 kDa Observed band size:60 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Serpin-F2-alpha-2-Antiplasmin-Antibody-001-Western-Blot-NBP2-89394-img0001.jpg "Western Blot: Serpin F2/alpha 2-Antiplasmin Antibody (001) [NBP2-89394] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:56 kDa Observed band size:60 kDa (We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: Serpin F2/alpha 2-Antiplasmin Antibody (001) [NBP2-89394] - Lane A:0.5 mg HepG2 Whole Cell Lysate 0.5 uL anti-SERPINF2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-SERPINF2 rabbit monoclonal antibody,at 1:1000 dilution Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 55 kDaObserved band size: 66 kDa](http://images.novusbio.com/fullsize/Serpin-F2-alpha-2-Antiplasmin-Antibody-001-Immunoprecipitation-NBP2-89394-img0002.jpg)
