Products Related to Gene-ID: 5133

156-B7) is coated at 0.5 µg/mL, 100 µL/well, Biotinylated Recombinant Human PD-1 Fc Chimera (Catalog # BT1086) binds with an ED₅₀ of 10.0-100 ng/mL." class="big_lightbox"> 156-B7) is coated at 0.5 µg/mL, 100 µL/well, Biotinylated Recombinant Human PD-1 Fc Chimera (Catalog # BT1086) binds with an ED₅₀ of 10.0-100 ng/mL." alt="When Recombinant Human B7-H1/PD-L1 Fc Chimera (156-B7) is coated at 0.5 µg/mL, 100 µL/well, Biotinylated Recombinant Human PD-1 Fc Chimera (Catalog # BT1086) binds with an ED₅₀ of 10.0-100 ng/mL." class="big_thumb" />

F0050), which does not stain the cells in the absence of recombinant protein (B)." class="big_lightbox"> F0050), which does not stain the cells in the absence of recombinant protein (B)." class="big_thumb" />

F0101B). Quadrant markers were set based on Mouse IgG1 Isotype Control (MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox"> F0101B). Quadrant markers were set based on Mouse IgG1 Isotype Control (MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol. " alt="HEK293 human embryonic kidney cell line transfected with (A) human PD-1 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human PD-1 Monoclonal Antibody (Catalog # MAB10868) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG1 Isotype Control (MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />

156-B7) to immobilized Recombinant Human PD‑1 His-tagged Protein (8986-PD) coated at 1 µg/mL (100 µL/well). At 10 µg/mL, this antibody will block >90% of the binding." class="big_lightbox"> 156-B7) to immobilized Recombinant Human PD‑1 His-tagged Protein (8986-PD) coated at 1 µg/mL (100 µL/well). At 10 µg/mL, this antibody will block >90% of the binding." class="big_thumb" />

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox"> DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="PD1 Antibody was detected in immersion fixed paraffin-embedded sections of human Tonsil using Rabbit Anti-Human PD1, Monoclonal Antibody (Catalog # MAB11638) at 15ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

HAF008). A specific band was detected for PD-1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for PD-1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NS0 mouse myeloma cell line mock transfected or transfected with human PD-1. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Cynomolgus Monkey PD-1 Monoclonal Antibody (Catalog # MAB10470) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PD-1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

1019-B7) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Mouse PD-1 His protein (Catalog # 9047-PD) was measured at a concentration range between 1.53 nM and 1.56 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.539 uM." class="big_lightbox"> 1019-B7) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Mouse PD-1 His protein (Catalog # 9047-PD) was measured at a concentration range between 1.53 nM and 1.56 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.539 uM." alt="Recombinant Mouse PD-L1/B7-H1 Fc protein (1019-B7) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Mouse PD-1 His protein (Catalog # 9047-PD) was measured at a concentration range between 1.53 nM and 1.56 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.539 uM." class="big_thumb" />

VC001. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic CTS013. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents Protocol." class="big_lightbox"> VC001. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic CTS013. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents Protocol." alt="PD‑1 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human PD‑1 Monoclonal Antibody (Catalog # MAB10865) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody VC001. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic CTS013. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents Protocol." class="big_thumb" />

156-B7) is immobilized at 1 µg/mL, 100 µg/well, Recombinant Human PD-1 Fc Chimera Avi-tag (Catalog # AVI1086) binds with an ED₅₀ of 0.01-0.09 µg/mL." class="big_lightbox"> 156-B7) is immobilized at 1 µg/mL, 100 µg/well, Recombinant Human PD-1 Fc Chimera Avi-tag (Catalog # AVI1086) binds with an ED₅₀ of 0.01-0.09 µg/mL." alt="When Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) is immobilized at 1 µg/mL, 100 µg/well, Recombinant Human PD-1 Fc Chimera Avi-tag (Catalog # AVI1086) binds with an ED₅₀ of 0.01-0.09 µg/mL." class="big_thumb" />