Products Related to Gene-ID: 5133

HAF017). Specific bands were detected for PD-1 at approximately 40-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). Specific bands were detected for PD-1 at approximately 40-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysate of human thymus tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PD-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for PD-1 at approximately 40-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF017). Specific bands were detected for PD-1 at approximately 40-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). Specific bands were detected for PD-1 at approximately 40-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0107) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0107) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

AF1086) at 1ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cell surface. " class="big_lightbox"> AF1086) at 1ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cell surface. " class="big_thumb" />

1086-PD) was serially diluted and captured by Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1086-PD) was serially diluted and captured by Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1086-PD) was serially diluted and captured by Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1086-PD) was serially diluted and captured by Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

156-B7) binds with a typical ED₅₀ of 0.15-0.75 µg/mL." class="big_lightbox"> 156-B7) binds with a typical ED₅₀ of 0.15-0.75 µg/mL." class="big_thumb" />

HAF017). A specific band was detected for PD-1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for PD-1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of EL-4 mouse lymphoblast cell line and CTLL-2 mouse cytotoxic T cell line (negative control). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse PD-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1021) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PD-1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF017). A specific band was detected for PD-1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for PD-1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox"> Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

F0108). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> F0108). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

HAF007). A specific band was detected for PD-1 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF007). A specific band was detected for PD-1 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with 1 µg/mL PHA for 5 days. PVDF Membrane was probed with 1 µg/mL of Human PD-1 Monoclonal Antibody (Catalog # MAB1086) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PD-1 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

8986-PD) is immobilized at 1 µg/mL (100 µL/well), the concentration of recombinant human PD-L1 (B7-H1) Fc Chimera (156-B7) that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL." class="big_lightbox"> 8986-PD) is immobilized at 1 µg/mL (100 µL/well), the concentration of recombinant human PD-L1 (B7-H1) Fc Chimera (156-B7) that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL." class="big_thumb" />

156-B7) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Human PD-1 His protein (Catalog # 8986-PD) was measured at a concentration range between 6.0 nM and 3.05 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.70 uM." class="big_lightbox"> 156-B7) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Human PD-1 His protein (Catalog # 8986-PD) was measured at a concentration range between 6.0 nM and 3.05 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.70 uM." class="big_thumb" />

1224-PL) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Human PD-1 His protein (Catalog # 8986-PD) was measured at a concentration range between 6.0 nM and 3.05 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.274 uM." class="big_lightbox"> 1224-PL) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Human PD-1 His protein (Catalog # 8986-PD) was measured at a concentration range between 6.0 nM and 3.05 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.274 uM." class="big_thumb" />

AVI9049) was immobilized on a Biacore Sensor Chip CM5 via the Avi-tag biotin, and binding to Recombinant Human PD-1 His protein (Catalog # 8986-PD) was measured at a concentration range between 3.2 nM and 13.2 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.122 µM." class="big_lightbox"> AVI9049) was immobilized on a Biacore Sensor Chip CM5 via the Avi-tag biotin, and binding to Recombinant Human PD-1 His protein (Catalog # 8986-PD) was measured at a concentration range between 3.2 nM and 13.2 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.122 µM." class="big_thumb" />

1019-B7) binds with an ED₅₀ of 0.16-0.8 µg/mL." class="big_lightbox"> 1019-B7) binds with an ED₅₀ of 0.16-0.8 µg/mL." alt="When Recombinant Mouse PD-1 Fc Chimera (Catalog # 1021-PD) is coated at 1 µg/mL, Recombinant Mouse B7-H1/PD-L1 Fc Chimera (Catalog # 1019-B7) binds with an ED₅₀ of 0.16-0.8 µg/mL." class="big_thumb" />

HAF018). Specific bands were detected for PD-1 at approximately 40-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). Specific bands were detected for PD-1 at approximately 40-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human PD-1. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PD-1 Monoclonal Antibody (Catalog # MAB10861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for PD-1 at approximately 40-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0101B) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # MAB002)." class="big_lightbox"> F0101B) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # MAB002)." class="big_thumb" />

BT156) binds to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP. (A) Binding is completely blocked by 2.5 µg/mL of Mouse Anti-Human PD-1 Monoclonal Antibody (Catalog # MAB10861) but not by (B) 2.5 µg/mL of Mouse IgG1 Isotype Control (MAB002). Protein binding was detected by staining cells with Streptavidin-APC (F0050). " class="big_lightbox"> BT156) binds to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP. (A) Binding is completely blocked by 2.5 µg/mL of Mouse Anti-Human PD-1 Monoclonal Antibody (Catalog # MAB10861) but not by (B) 2.5 µg/mL of Mouse IgG1 Isotype Control (MAB002). Protein binding was detected by staining cells with Streptavidin-APC (F0050). " class="big_thumb" />