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![Western Blot: PD-1 Antibody (4F12) [NBP1-04328] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCD1 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate, Lane 2.: Raji cell lysate](http://images.novusbio.com/fullsize/PD-1-Antibody-4F12-Western-Blot-NBP1-04328-img0005.jpg "Western Blot: PD-1 Antibody (4F12) [NBP1-04328] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCD1 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate, Lane 2.: Raji cell lysate")
![Immunocytochemistry/Immunofluorescence: PD-1 Antibody (4F12) [NBP1-04328] - HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PDCD1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/PD-1-Antibody-4F12-Immunocytochemistry-Immunofluorescence-NBP1-04328-img0004.jpg)
![Flow Cytometry: PD-1 Antibody (4F12) [NBP1-04328] - Flow cytometry analysis of PDCD1 in HeLa cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.](http://images.novusbio.com/fullsize/PD-1-Antibody-4F12-Flow-Cytometry-NBP1-04328-img0003.jpg)
![Western Blot: PD1 Peptide [NBP1-77276PEP] - blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37C.](http://images.novusbio.com/fullsize/PD1-Peptide-Western-Blot-NBP1-77276PEP-img0001.jpg "Western Blot: PD1 Peptide [NBP1-77276PEP] - blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37C.")