| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13657-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93990-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03704-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57419-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97595-50ul | |||
![Immunocytochemistry/Immunofluorescence: Ninein Antibody [NBP2-13657] - Staining of human cell line U-251 MG shows localization to nucleoli and centrosome.](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13657-img0009.jpg "Immunocytochemistry/Immunofluorescence: Ninein Antibody [NBP2-13657] - Staining of human cell line U-251 MG shows localization to nucleoli and centrosome.")
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-13657] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-13657-img0013.jpg)
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-13657] - Staining of human tonsil shows moderate cytoplasmic and membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-13657-img0010.jpg)
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-13657] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-13657-img0011.jpg)
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-13657] - Staining of human stomach shows moderate cytoplasmic positivity in parietal cells.](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-13657-img0012.jpg)
![Western Blot: Ninein Antibody (PSH03-46) [NBP3-32652] - Western blot analysis of Ninein on Ninein recombinant protein with Rabbit anti-Ninein antibody (NBP3-32652) at 1/2,000 dilution. Lysates/proteins at 20 ng/Lane. Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32652) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32652_rabbit-ninein-mab-psh03-46-278202416285254.jpg "Western Blot: Ninein Antibody (PSH03-46) [NBP3-32652] - Western blot analysis of Ninein on Ninein recombinant protein with Rabbit anti-Ninein antibody (NBP3-32652) at 1/2,000 dilution. Lysates/proteins at 20 ng/Lane. Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32652) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Ninein Antibody (PSH03-46) [NBP3-32652] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Ninein antibody (NBP3-32652) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32652) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32652_rabbit-ninein-mab-psh03-46-298202414161899.jpg)
![Immunocytochemistry/ Immunofluorescence: Ninein Antibody (PSH03-46) [NBP3-32652] - Immunocytochemistry analysis of NIH/3T3 cells labeling Ninein with Rabbit anti-Ninein antibody (NBP3-32652) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ninein antibody (NBP3-32652) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32652_rabbit-ninein-mab-psh03-46-29202410334027.jpg)
![Flow Cytometry: Ninein Antibody (PSH03-46) [NBP3-32652] - Flow cytometric analysis of NIH/3T3 cells labeling Ninein. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32652, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32652_rabbit-ninein-mab-psh03-46-29202416372136.jpg)
![Western Blot: Ninein Antibody [NBP2-93990] - Analysis of extracts of Jurkat cells, using Ninein .Exposure time: 90s.](http://images.novusbio.com/fullsize/Ninein-Antibody-Western-Blot-NBP2-93990-img0005.jpg "Western Blot: Ninein Antibody [NBP2-93990] - Analysis of extracts of Jurkat cells, using Ninein .Exposure time: 90s.")
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-93990] - Paraffin-embedded rat kidney using Ninein .](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-93990-img0004.jpg)
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-93990] - Paraffin-embedded human stomach using Ninein .](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-93990-img0002.jpg)
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-93990] - Paraffin-embedded mouse lung using Ninein .](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-93990-img0003.jpg)
![Western Blot: Ninein Antibody [NB100-74631] - DGAT1 inhibition decreases ncMTOC-related proteins in prostate cancer cells. gamma-tubulin (48 kD), ninein (243 kD), GM130 (140 kD), and CETN1 (20 kD) levels were analyzed by western blot in PC-3 control (CTR) and treated with 1 uM DGAT1 inhibitor or 10 nM PEDF. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-39537-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ninein-Antibody-Western-Blot-NB100-74631-img0003.jpg "Western Blot: Ninein Antibody [NB100-74631] - DGAT1 inhibition decreases ncMTOC-related proteins in prostate cancer cells. gamma-tubulin (48 kD), ninein (243 kD), GM130 (140 kD), and CETN1 (20 kD) levels were analyzed by western blot in PC-3 control (CTR) and treated with 1 uM DGAT1 inhibitor or 10 nM PEDF. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-39537-z), licensed under a CC-BY license.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Ninein Antibody [NB100-74631] - Detection of Human and Mouse NINEIN on HeLa whole cell lysate using NB100-74631. NINEIN was also immunoprecipitated by another rabbit anti-NINEIN antibody.](http://images.novusbio.com/fullsize/Ninein-Antibody-Western-Blot-NB100-74631-img0002.jpg)
![Western Blot: Ninein Antibody [NBP3-03704] - Analysis of extracts of various cell lines, using Ninein antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/Ninein-Antibody-Western-Blot-NBP3-03704-img0002.jpg "Western Blot: Ninein Antibody [NBP3-03704] - Analysis of extracts of various cell lines, using Ninein antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunocytochemistry/Immunofluorescence: Ninein Antibody [NBP2-57419] - Staining of human cell line RT4 shows localization to nucleus, nucleoli & centrosome.](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57419-img0001.jpg "Immunocytochemistry/Immunofluorescence: Ninein Antibody [NBP2-57419] - Staining of human cell line RT4 shows localization to nucleus, nucleoli & centrosome.")
![Western Blot: Ninein Antibody (PSH03-23) [NBP3-32651] - Western blot analysis of Ninein on different lysates with Rabbit anti-Ninein antibody (NBP3-32651) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: HT-29 cell lysate Lane 4: MEF cell lysate Lane 5: Human lung tissue lysate Lane 6: Mouse lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 243 kDa Observed band size: 270 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32651) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32651_rabbit-ninein-mab-psh03-23-27820241628520.jpg "Western Blot: Ninein Antibody (PSH03-23) [NBP3-32651] - Western blot analysis of Ninein on different lysates with Rabbit anti-Ninein antibody (NBP3-32651) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: HT-29 cell lysate Lane 4: MEF cell lysate Lane 5: Human lung tissue lysate Lane 6: Mouse lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 243 kDa Observed band size: 270 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32651) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-97595] - Immunochemical staining of human Ninein in human corpus uteri with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-97595-img0002.jpg "Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-97595] - Immunochemical staining of human Ninein in human corpus uteri with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-97595] - Immunochemical staining of human Ninein in human colon with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-97595-img0001.jpg)