| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86960-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7647-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7647-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-66597-250ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20946 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08435 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26171-50ul | |||
![Western Blot: LEF1 Antibody [NBP1-86960] - Analysis in human cell lines SK-MEL-30 and A-549 using anti-LEF1 antibody. Corresponding LEF1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.](http://images.novusbio.com/fullsize/LEF1-Antibody-Western-Blot-NBP1-86960-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: LEF1 Antibody [NBP1-86960] - Analysis in human cell lines SK-MEL-30 and A-549 using anti-LEF1 antibody. Corresponding LEF1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: LEF1 Antibody [NBP1-86960] - Analysis in control (vector only transfected HEK293T lysate) and LEF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/LEF1-Antibody-Western-Blot-NBP1-86960-img0025.jpg)
![Immunocytochemistry/Immunofluorescence: LEF1 Antibody [NBP1-86960] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LEF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86960-img0024.jpg)
![Immunohistochemistry-Paraffin: LEF1 Antibody [NBP1-86960] - Staining of human oral mucosa shows moderate nuclear positivity in subset of squamous epithelial cells.](http://images.novusbio.com/fullsize/LEF1-Antibody-Immunohistochemistry-Paraffin-NBP1-86960-img0018.jpg)
![Immunohistochemistry-Paraffin: LEF1 Antibody [NBP1-86960] - Staining of human lymph node shows moderate to strong nuclear positivity in non - germinal center cells.](http://images.novusbio.com/fullsize/LEF1-Antibody-Immunohistochemistry-Paraffin-NBP1-86960-img0017.jpg)
![Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - LEF1 monoclonal antibody (M01), clone 3H5 Analysis of LEF1 expression in MES-SA/Dx5.](http://images.novusbio.com/fullsize/LEF1-Antibody-3H5-Western-Blot-H00051176-M01-img0009.jpg "Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - LEF1 monoclonal antibody (M01), clone 3H5 Analysis of LEF1 expression in MES-SA/Dx5.")
![Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - Analysis of LEF1 over-expressed 293 cell line, cotransfected with LEF1 Validated Chimera RNAi ( Cat # H00051176-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with LEF1 monoclonal antibody (M01), clone 3H5 (Cat # H00051176-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/LEF1-Antibody-3H5-Western-Blot-H00051176-M01-img0006.jpg)
![Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - LEF1 monoclonal antibody (M01), clone 3H5. Analysis of LEF1 expression in HL-60.](http://images.novusbio.com/fullsize/LEF1-Antibody-3H5-Western-Blot-H00051176-M01-img0008.jpg)
![Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - Analysis of LEF1 expression in transfected 293T cell line by LEF1 monoclonal antibody (M01), clone 3H5.Lane 1: LEF1 transfected lysate(44.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/LEF1-Antibody-3H5-Western-Blot-H00051176-M01-img0010.jpg)
![Sandwich ELISA: LEF1 Antibody (3H5) [H00051176-M01] - Detection limit for recombinant GST tagged LEF1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/LEF1-Antibody-3H5-Sandwich-ELISA-H00051176-M01-img0007.jpg)
![Western Blot: LEF1 Antibody [NB100-41374] - Staining of K562 nuclear cell lysate (A) + peptide (B). Antibody at 2 ug/mL. 35 ug protein in RIPA buffer. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/LEF1-Antibody-Western-Blot-NB100-41374-img0001.jpg "Western Blot: LEF1 Antibody [NB100-41374] - Staining of K562 nuclear cell lysate (A) + peptide (B). Antibody at 2 ug/mL. 35 ug protein in RIPA buffer. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: LEF1 Antibody [NB100-41374] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/LEF1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41374-img0003.jpg)
![Flow Cytometry: LEF1 Antibody [NB100-41374] - Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/LEF1-Antibody-Flow-Cytometry-NB100-41374-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: LEF1 Antibody [NB100-41374] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/LEF1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41374-img0002.jpg)
![Western Blot: LEF1 Antibody (JE52-28) [NBP3-32527] - Western blot analysis of LEF1 on different lysates with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (negative) (20 ug/Lane) Lane 3: Mouse thymus tissue lysate (40 ug/Lane) Lane 4: Rat thymus tissue lysate (40 ug/Lane) Predicted band size: 44 kDa Observed band size: 35-55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32527) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32527_rabbit-lef1-mab-je52-28-278202415212459.jpg "Western Blot: LEF1 Antibody (JE52-28) [NBP3-32527] - Western blot analysis of LEF1 on different lysates with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (negative) (20 ug/Lane) Lane 3: Mouse thymus tissue lysate (40 ug/Lane) Lane 4: Rat thymus tissue lysate (40 ug/Lane) Predicted band size: 44 kDa Observed band size: 35-55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32527) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LEF1 Antibody (JE52-28) [NBP3-32527] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32527) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32527_rabbit-lef1-mab-je52-28-298202414333615.jpg)
![Immunocytochemistry/ Immunofluorescence: LEF1 Antibody (JE52-28) [NBP3-32527] - Immunocytochemistry analysis of Jurkat (positive) and HeLa (negative) labeling LEF1 with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32527_rabbit-lef1-mab-je52-28-2920241023422.jpg)
![Western Blot: LEF1 Antibody (5A3) [H00051176-M03] - Analysis of LEF1 expression in HepG2 (Cat # L019V1).](http://images.novusbio.com/fullsize/LEF1-Antibody-5A3-Western-Blot-H00051176-M03-img0007.jpg "Western Blot: LEF1 Antibody (5A3) [H00051176-M03] - Analysis of LEF1 expression in HepG2 (Cat # L019V1).")
![Immunocytochemistry/Immunofluorescence: LEF1 Antibody (5A3) [H00051176-M03] - Analysis of monoclonal antibody to LEF1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/LEF1-Antibody-5A3-Immunocytochemistry-Immunofluorescence-H00051176-M03-img0006.jpg)
![Western Blot: LEF1 Antibody (5A3) [H00051176-M03] - Analysis of LEF1 expression in Jurkat (Cat # L017V1).](http://images.novusbio.com/fullsize/LEF1-Antibody-5A3-Western-Blot-H00051176-M03-img0008.jpg)
![ELISA: LEF1 Antibody (5A3) [H00051176-M03] - Detection limit for recombinant GST tagged LEF1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/LEF1-Antibody-5A3-ELISA-H00051176-M03-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: LEF1 Antibody (REMB6) [NBP2-66597] - Figure 1. Immunofluorescence staining of Cos-1 cells transfected with (top to bottom) full length hLEF1, a LEF1 mutant missing the nuclear localization signal, TCF-4 and TCF -1. Cells were stained with Novus Biologicals' LEF1 antibody, clone REMB6 cat. # T105M and visualized with FITC conjugated mouse IgG. DAPI was used to detect all nuclei. The LEF/TCF/HMG, clone REMB6 recognizes an epitope in the highly conserved HMG DNA binding domain.](http://images.novusbio.com/fullsize/nbp2-66597_mouse-monoclonal-lef1-antibody-remb6-811202420592164.jpg "Immunocytochemistry/ Immunofluorescence: LEF1 Antibody (REMB6) [NBP2-66597] - Figure 1. Immunofluorescence staining of Cos-1 cells transfected with (top to bottom) full length hLEF1, a LEF1 mutant missing the nuclear localization signal, TCF-4 and TCF -1. Cells were stained with Novus Biologicals' LEF1 antibody, clone REMB6 cat. # T105M and visualized with FITC conjugated mouse IgG. DAPI was used to detect all nuclei. The LEF/TCF/HMG, clone REMB6 recognizes an epitope in the highly conserved HMG DNA binding domain.")
![Immunohistochemistry-Paraffin: LEF1 Antibody (LEF1/341R) [NBP3-07769] - IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Strong nuclear staining of non-germinal center cells using LEF1 antibody (LEF1/341R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 : HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/LEF1-Antibody-LEF1-341R-Immunohistochemistry-Paraffin-NBP3-07769-img0002.jpg "Immunohistochemistry-Paraffin: LEF1 Antibody (LEF1/341R) [NBP3-07769] - IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Strong nuclear staining of non-germinal center cells using LEF1 antibody (LEF1/341R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 : HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: LEF1 Antibody (LEF1/341R) [NBP3-07769] - IHC analysis of formalin-fixed, paraffin-embedded human lymph node. Strong nuclear staining of non-germinal center cells using LEF1( LEF1/341R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 : HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/LEF1-Antibody-LEF1-341R-Immunohistochemistry-Paraffin-NBP3-07769-img0001.jpg)
![Immunohistochemistry: LEF1 Antibody (13B7) [NBP3-26171] - Image of LEF1 Antibody (13B7) diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB.](http://images.novusbio.com/fullsize/nbp3-26171_rabbit-lef1-mab-13b7-26220241547562.jpg "Immunohistochemistry: LEF1 Antibody (13B7) [NBP3-26171] - Image of LEF1 Antibody (13B7) diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB.")
![Immunohistochemistry: LEF1 Antibody (13B7) [NBP3-26171] - Image of LEF1 Antibody (13B7) diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB.](http://images.novusbio.com/fullsize/nbp3-26171_rabbit-lef1-mab-13b7-26220241535579.jpg)