| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73086 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88217-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38066-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97441-50ul | |||
![Western Blot: Nucleobindin 1 Antibody (1A5) [NBP2-01446] - Analysis of extracts (10ug) from 9 Human tissue by using anti-NUCB1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-1A5-Western-Blot-NBP2-01446-img0010.jpg "Western Blot: Nucleobindin 1 Antibody (1A5) [NBP2-01446] - Analysis of extracts (10ug) from 9 Human tissue by using anti-NUCB1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).")
![Immunocytochemistry/Immunofluorescence: Nucleobindin 1 Antibody (OTI1A5) [NBP2-01446] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Nucleobindin 1.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-1A5-Immunocytochemistry-Immunofluorescence-NBP2-01446-img0006.jpg)
![Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody (1A5) [NBP2-01446] - Staining of paraffin-embedded Human endometrium tissue using anti-Nucleobindin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-1A5-Immunohistochemistry-Paraffin-NBP2-01446-img0008.jpg)
![Flow Cytometry: Nucleobindin 1 Antibody (1A5) [NBP2-01446] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Nucleobindin 1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-1A5-Flow-Cytometry-NBP2-01446-img0001.jpg)
![Western Blot: Nucleobindin 1 Antibody (1A5) [NBP2-01446] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nucleobindin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nucleobindin 1.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-1A5-Western-Blot-NBP2-01446-img0002.jpg)
![Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody (1A5) [NBP2-01446] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Nucleobindin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-1A5-Immunohistochemistry-Paraffin-NBP2-01446-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleobindin 1 Antibody [NBP1-88217] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88217-img0014.jpg "Immunocytochemistry/Immunofluorescence: Nucleobindin 1 Antibody [NBP1-88217] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP1-88217] - Staining in human small intestine and pancreas tissues using NBP1-88217 antibody. Corresponding NUCB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88217-img0020.jpg)
![Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP1-88217] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88217-img0016.jpg)
![Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP1-88217] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88217-img0017.jpg)
![Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP1-88217] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88217-img0018.jpg)
![Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP1-88217] - Staining of human small intestine shows strong positivity in Golgi apparatus in glandular cells.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88217-img0019.jpg)
![Nucleobindin 1 antibody [HL2532] detects Nucleobindin 1 protein at Golgi apparatus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nucleobindin 1 stained by Nucleobindin 1 antibody [HL2532] (NBP3-48694) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48694_rabbit-nucleobindin-1-mab-hl2532-immunocytochemistry-immunofluorescence-141120251824334.jpg "Nucleobindin 1 antibody [HL2532] detects Nucleobindin 1 protein at Golgi apparatus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nucleobindin 1 stained by Nucleobindin 1 antibody [HL2532] (NBP3-48694) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .")
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Nucleobindin 1 antibody [HL2532] (NBP3-48694) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48694_rabbit-nucleobindin-1-mab-hl2532-western-blot-141120251831215.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Nucleobindin 1 antibody [HL2532] (NBP3-48694) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48694_rabbit-nucleobindin-1-mab-hl2532-western-blot-1411202518372711.jpg)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Nucleobindin 1 antibody [HL2532] (NBP3-48694) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48694_rabbit-nucleobindin-1-mab-hl2532-western-blot-141120251843313.jpg)
![Nucleobindin 1 antibody [HL2532] detects secreted Nucleobindin 1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Nucleobindin 1 stained by Nucleobindin 1 antibody [HL2532] (NBP3-48694) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48694_rabbit-nucleobindin-1-mab-hl2532-immunohistochemistry-paraffin-1411202518322921.jpg)
![Nucleobindin 1 antibody [HL2532] detects secreted Nucleobindin 1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Nucleobindin 1 stained by Nucleobindin 1 antibody [HL2532] (NBP3-48694) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48694_rabbit-nucleobindin-1-mab-hl2532-immunohistochemistry-paraffin-14112025182607.jpg)
![Western Blot: Nucleobindin 1 Antibody [NBP2-19601] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-Western-Blot-NBP2-19601-img0002.jpg "Western Blot: Nucleobindin 1 Antibody [NBP2-19601] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Nucleobindin 1 Antibody [NBP2-19601] - Nucleobindin 1 antibody detects Nucleobindin 1 protein at Golgi apparatus by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nucleobindin 1 protein stained by Nucleobindin 1 antibody diluted at 1:500. Red: FIS1, a mitochondria marker, stained by FIS1 antibody diluted at 1:20000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19601-img0006.jpg)
![Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP2-19601] - Mouse kidney. Nucleobindin 1 antibody [N3C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-19601-img0007.jpg)
![Western Blot: Nucleobindin 1 Antibody [NBP2-19601] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-Western-Blot-NBP2-19601-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleobindin 1 Antibody [NBP2-19601] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19601-img0004.jpg)
![Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP2-19601] - Mouse kidney. Nucleobindin 1 antibody [N3C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-19601-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Nucleobindin 1 Antibody [NBP3-38066] - Immunofluorescence analysis of L929 cells using [KO Validated] Nucleobindin 1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38066_rabbit-nucleobindin-1-pab-912202415143681.jpg "Immunocytochemistry/ Immunofluorescence: Nucleobindin 1 Antibody [NBP3-38066] - Immunofluorescence analysis of L929 cells using [KO Validated] Nucleobindin 1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: Nucleobindin 1 Antibody [NBP3-38066] - Western blot analysis of various lysates using [KO Validated] Nucleobindin 1 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38066_rabbit-nucleobindin-1-pab-91220241415435.jpg)
![Immunocytochemistry/ Immunofluorescence: Nucleobindin 1 Antibody [NBP3-38066] - Immunofluorescence analysis of U-2 OS cells using [KO Validated] Nucleobindin 1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38066_rabbit-nucleobindin-1-pab-912202415171164.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Nucleobindin 1 Antibody [NBP3-38066] - Western Blot analysis of lysates from wild type (WT) and Nucleobindin 1 knockout (KO) 293T cells, using [KO Validated] Nucleobindin 1 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-38066_rabbit-nucleobindin-1-pab-31120251704779.jpg%3Ft=1709198900)
![Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP2-97441] - Immunochemical staining of human Nucleobindin 1 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-97441-img0002.jpg "Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP2-97441] - Immunochemical staining of human Nucleobindin 1 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunocytochemistry/Immunofluorescence: Nucleobindin 1 Antibody [NBP2-97441] - Immunofluorescence staining of Nucleobindin 1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97441 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97441_rabbit-polyclonal-nucleobindin-1-antibody-235202521525020.png)
![Immunoprecipitation:Nucleobindin 1 AntibodyNBP3-29848] -Detection of human Nucleobindin 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nucleobindin 1 antibody NBP3-29848 (lot NBP3-29848-1) used for IP at 6 µg per reaction. Nucleobindin 1 was also immunoprecipitated by rabbit anti-Nucleobindin 1.. For blotting immunoprecipitated Nucleobindin 1, NBP3-29848 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29848_rabbit-nucleobindin-1-pab-3012202413324213.jpg "Immunoprecipitation:Nucleobindin 1 AntibodyNBP3-29848] -Detection of human Nucleobindin 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nucleobindin 1 antibody NBP3-29848 (lot NBP3-29848-1) used for IP at 6 µg per reaction. Nucleobindin 1 was also immunoprecipitated by rabbit anti-Nucleobindin 1.. For blotting immunoprecipitated Nucleobindin 1, NBP3-29848 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: Nucleobindin 1 Overexpression Lysate (Adult Normal) [NBL1-13848] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nucleobindin 1.](http://images.novusbio.com/fullsize/Nucleobindin-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13848-img0002.jpg "Western Blot: Nucleobindin 1 Overexpression Lysate (Adult Normal) [NBL1-13848] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nucleobindin 1.")
![Immunoprecipitation:Nucleobindin 1 AntibodyNBP3-30115] -Detection of human Nucleobindin 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Nucleobindin 1 antibody NBP3-30115 (lot NBP3-30115-1) used for IP at 6 µg per reaction. Nucleobindin 1 was also immunoprecipitated by rabbit anti-Nucleobindin 1.. For blotting immunoprecipitated Nucleobindin 1, NBP3-30115 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30115_rabbit-nucleobindin-1-pab-3012202413124113.jpg "Immunoprecipitation:Nucleobindin 1 AntibodyNBP3-30115] -Detection of human Nucleobindin 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Nucleobindin 1 antibody NBP3-30115 (lot NBP3-30115-1) used for IP at 6 µg per reaction. Nucleobindin 1 was also immunoprecipitated by rabbit anti-Nucleobindin 1.. For blotting immunoprecipitated Nucleobindin 1, NBP3-30115 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")