| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37274-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB44881-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB4488F | |||
| Carrier Free | MAB4488-SP | |||
| Carrier Free | FAB4488S-100UG | |||
| Carrier Free | FAB4488R-100UG | |||
| Carrier Free | FAB4488N-100UG | |||
| Carrier Free | FAB4488G-100UG | |||
| Carrier Free | FAB4488V-100UG | |||
| Carrier Free | FAB4488U-100UG | |||
| Carrier Free | FAB4488T-100UG | |||
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mouse mAb against A431 (1) cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Western-Blot-NBP2-37274-img0004.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mouse mAb against A431 (1) cell lysate.")
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis of esophagus cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Immunohistochemistry-NBP2-37274-img0006.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mAb against human NT5E (AA: ) recombinant protein. (Expected MW is 26.6 kDa)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Western-Blot-NBP2-37274-img0002.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mAb against HEK293 (1) and NT5E (AA: 30-250)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Western-Blot-NBP2-37274-img0003.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis of bladder cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Immunohistochemistry-NBP2-37274-img0005.jpg)
![ELISA: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-ELISA-NBP2-37274-img0001.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (BLR054F) [NBP3-14679] - FFPE section of IMR-90 cells. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody used at 1:100. HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-BLR054F-Western-Blot-NBP3-14679-img0003.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (BLR054F) [NBP3-14679] - FFPE section of IMR-90 cells. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody used at 1:100. HRP-conjugated goat anti-rabbit IgG.")
![Immunocytochemistry: 5'-Nucleotidase/CD73 Antibody (BLR054F) [NBP3-14679] - FFPE section of IMR-90 cells. Rabbit anti-CD73 recombinant monoclonal antibody used at 1:100. HRP-conjugated goat anti-rabbit IgG Substrate DAB.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-BLR054F-Immunocytochemistry-NBP3-14679-img0002.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (BLR054F) [NBP3-14679] - FFPE section of human tonsil. Antibody: rabbit anti-CD73 recombinant monoclonal antibody used at 1:250. secondary: HRP- conjugated goat anti-rabbit igG](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-BLR054F-Immunohistochemistry-NBP3-14679-img0004.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (BLR054F) [NBP3-14679] - FFPE section of human head and neck squamous cell carcinoma. Rabbit anti-CD73 recombinant monoclonal antibody used at 1:500. HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-BLR054F-Immunohistochemistry-NBP3-14679-img0001.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Western blot analysis of 5'-Nucleotidase/CD73 on different lysates with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: U-87 MG cell lysate (no heat) Lysates/proteins at 30 ug/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32166) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-278202416285219.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Western blot analysis of 5'-Nucleotidase/CD73 on different lysates with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: U-87 MG cell lysate (no heat) Lysates/proteins at 30 ug/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32166) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Immunohistochemical analysis of paraffin-embedded NCI-H441 xenograft tissue with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32166) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-298202414304184.jpg)
![Immunocytochemistry/ Immunofluorescence: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Immunocytochemistry analysis of U-87 MG cells labeling 5'-Nucleotidase/CD73 with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-2920241192315.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP1-32951] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Western-Blot-NBP1-32951-img0006.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP1-32951] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-32951] - MDAMB231 xenograft, using CD73 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-Paraffin-NBP1-32951-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP1-32951] - Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32951_rabbit-polyclonal-5-nucleotidase-cd73-antibody-1610202419405720.jpg)
![Immunocytochemistry/ Immunofluorescence: 5'-Nucleotidase/CD73 Antibody (1E9) - Chimeric - BSA Free [NBP3-45121] - Immunofluorescence analysis of paraformaldehyde-fixed U251 cells on coverslips stained with NBP3-45121 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic, nuclear, and nuclear bodies staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-45121, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-48418_rabbit-tmub2-pab-azide-and-bsa-free-western-blot-222026031182.png "Immunocytochemistry/ Immunofluorescence: 5'-Nucleotidase/CD73 Antibody (1E9) - Chimeric - BSA Free [NBP3-45121] - Immunofluorescence analysis of paraformaldehyde-fixed U251 cells on coverslips stained with NBP3-45121 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic, nuclear, and nuclear bodies staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-45121, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.")
![Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (1E9) - Chimeric - BSA Free [NBP3-45121] - Paraformaldehyde-fixed U251 cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (negative control, black line) or NBP3-45121 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-45121_rabbit-5-nucleotidase-cd73-mab-1e9-chimeric-bsa-free-flow-cytometry-222026032589.png)