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![Western Blot: NF2/Merlin Antibody (PSH02-52) [NBP3-32590] - Western blot analysis of NF2/Merlin on different lysates with Rabbit anti-NF2/Merlin antibody (NBP3-32590) at 1/2,000 dilution. Lane 1: PC-3M cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: MDA-MB-468 cell lysate (20 ug/Lane) Lane 4: SH-SY5Y cell lysate (20 ug/Lane) Lane 5: HUVEC cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: Jurkat cell lysate (20 ug/Lane) Lane 8: MDA-MB-231 cell lysate (negative) (20 ug/Lane) Lane 9: COS-1 cell lysate (20 ug/Lane) Lane 10: Neuro-2a cell lysate (20 ug/Lane) Lane 11: NIH/3T3 cell lysate (20 ug/Lane) Lane 12: C6 cell lysate (20 ug/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute 41 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32590) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32590_rabbit-nf2-merlin-mab-psh02-52-278202415242628.jpg "Western Blot: NF2/Merlin Antibody (PSH02-52) [NBP3-32590] - Western blot analysis of NF2/Merlin on different lysates with Rabbit anti-NF2/Merlin antibody (NBP3-32590) at 1/2,000 dilution. Lane 1: PC-3M cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: MDA-MB-468 cell lysate (20 ug/Lane) Lane 4: SH-SY5Y cell lysate (20 ug/Lane) Lane 5: HUVEC cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: Jurkat cell lysate (20 ug/Lane) Lane 8: MDA-MB-231 cell lysate (negative) (20 ug/Lane) Lane 9: COS-1 cell lysate (20 ug/Lane) Lane 10: Neuro-2a cell lysate (20 ug/Lane) Lane 11: NIH/3T3 cell lysate (20 ug/Lane) Lane 12: C6 cell lysate (20 ug/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute 41 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32590) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody (PSH02-52) [NBP3-32590] - Immunocytochemistry analysis of HUVEC cells labeling NF2/Merlin with Rabbit anti-NF2/Merlin antibody (NBP3-32590) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF2/Merlin antibody (NBP3-32590) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32590_rabbit-nf2-merlin-mab-psh02-52-2920241192366.jpg)
![Flow Cytometry: NF2/Merlin Antibody (PSH02-52) [NBP3-32590] - Flow cytometric analysis of NIH/3T3 cells labeling NF2/Merlin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32590, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32590_rabbit-nf2-merlin-mab-psh02-52-29202416372197.jpg)
![Western Blot: NF2/Merlin Antibody (JE31-02) [NBP3-32589] - Western blot analysis of NF2/Merlin on different lysates with Rabbit anti-NF2/Merlin antibody (NBP3-32589) at 1/500 dilution. Lane 1: Hela cell lysate (10 ug/Lane) Lane 2: Mouse brain tissue lysate (20 ug/Lane) Lane 3: Rat brain tissue lysate (20 ug/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32589) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32589_rabbit-nf2-merlin-mab-je31-02-27820241631649.jpg "Western Blot: NF2/Merlin Antibody (JE31-02) [NBP3-32589] - Western blot analysis of NF2/Merlin on different lysates with Rabbit anti-NF2/Merlin antibody (NBP3-32589) at 1/500 dilution. Lane 1: Hela cell lysate (10 ug/Lane) Lane 2: Mouse brain tissue lysate (20 ug/Lane) Lane 3: Rat brain tissue lysate (20 ug/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32589) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Merlin Overexpression Lysate (Adult Normal) [NBL1-13606] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Merlin.](http://images.novusbio.com/fullsize/Merlin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13606-img0002.jpg "Western Blot: Merlin Overexpression Lysate (Adult Normal) [NBL1-13606] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Merlin.")
![Western Blot: NF2/Merlin Antibody [NBP2-99421] - Anti-NF2/Merlin rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 69 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Western-Blot-NBP2-99421-img0002.jpg "Western Blot: NF2/Merlin Antibody [NBP2-99421] - Anti-NF2/Merlin rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 69 kDa (We are unsure as to the identity of these extra bands).")
![ELISA: Human NF2/Merlin ELISA Kit (Colorimetric) [NBP3-39130] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39130_human-nf2-merlin-elisa-kit-colorimetric-2110202415305235.png "ELISA: Human NF2/Merlin ELISA Kit (Colorimetric) [NBP3-39130] - Standard Curve Reference")
![ELISA: Human NF2/Merlin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39131] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39131_human-nf2-merlin-ready-to-use-elisa-kit-colorimetric-2110202415403158.png "ELISA: Human NF2/Merlin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39131] - Standard Curve Reference")