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![ELISA: SARS-CoV-2 Spike Recombinant Protein [NBP3-14666] - SARS-CoV-2 Spike Recombinant Protein Binding Activity. SARS-CoV-2 Spike Recombinant Protein (Catalog # NBP3-14666) binds Recombinant Human ACE-2 His-tag (R&D Systems, Catalog # 933-ZN) in a functional ELISA.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Recombinant-Protein-ELISA-NBP3-14666-img0001.jpg "ELISA: SARS-CoV-2 Spike Recombinant Protein [NBP3-14666] - SARS-CoV-2 Spike Recombinant Protein Binding Activity. SARS-CoV-2 Spike Recombinant Protein (Catalog # NBP3-14666) binds Recombinant Human ACE-2 His-tag (R&D Systems, Catalog # 933-ZN) in a functional ELISA.")
![ELISA: SARS-CoV-2 Spike Recombinant Protein [NBP3-14666] - SARS-CoV-2 Spike Recombinant Protein Binding Activity. SARS-CoV-2 Spike Recombinant Protein (Catalog # NBP3-14666) binds Recombinant Human ACE-2 His-tag (R&D Systems, Catalog # 933-ZN) in a functional ELISA.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Recombinant-Protein-ELISA-NBP3-14666-img0002.jpg)
![ELISA: Recombinant SARS-CoV-2 Spike S1 His (C-Term) Protein [NBP2-90985] - Immobilized Recombinant SARS-COV-2 Spike S1 at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 0.5-8.7 ng/mL.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-S1-His-C-Term-Protein-ELISA-NBP2-90985-img0002.jpg "ELISA: Recombinant SARS-CoV-2 Spike S1 His (C-Term) Protein [NBP2-90985] - Immobilized Recombinant SARS-COV-2 Spike S1 at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 0.5-8.7 ng/mL.")
![HPLC: Recombinant SARS-CoV-2 Spike S1 His (C-Term) Protein [NBP2-90985] - The purity of SARS-COV-2 Spike S1 Protein with His tag (Cat.NBP2-90985) was greater than 95% as determined by SEC-HPLC.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-S1-His-C-Term-Protein-HPLC-NBP2-90985-img0003.jpg)
![SDS-Page: Recombinant SARS-CoV-2 Spike S1 His (C-Term) Protein [NBP2-90985] - Recombinant SARS-COV-2 Spike S1 Protein with His tag was determined by SDS-PAGE with Coomassie Blue, showing a band at 110-130 kDa.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-S1-His-C-Term-Protein-SDS-Page-NBP2-90985-img0005.jpg)
![Surface Plasmon Resonance: Recombinant SARS-CoV-2 Spike S1 His (C-Term) Protein [NBP2-90985] - Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 11.4 nM as determined in a SPR assay.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-S1-His-C-Term-Protein-Surface-Plasmon-Resonance-NBP2-90985-img0004.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) or goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-212024939162.jpg "ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) or goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Indirect ELISA analysis was performed by coating the plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 Alpha variant; B.1.351 Beta variant; P.1 Gamma variant; B1.617.2 Delta variant; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) (28.78-0.45 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-212024934276.jpg)
![Functional (Inhibition): SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Inhibition analysis of immobilized recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) (coated at 2 ug/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (1000 ng/mL) binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (0.09-66.67 nM). Bound was detected by Goat Anti-Mouse IgG antibody (HRP) (1:10000).](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-2120249432894.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.4/ BA.5 Omicron variant; Omicron / XBB variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Please notice that NBP3-25671 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-21202494089.jpg)