| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 10549-CV-01M | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB10557-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB10850-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26918-0.025mg | |||
![ELISA: SARS-CoV-2 Spike Antibody (CR3022) [NBP2-90980] - Binding curve of SARS-CoV-2 Spike Antibody (CR3022) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 of various origin. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line), SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) and SARS Coronavirus Spike Glycoprotein (S1), His-Tag (HEK293 cells; blue line) (Native Antigen) at concentrations of 5 ug/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using SARS-CoV-2 Spike Antibody (CR3022). For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-CR3022-ELISA-NBP2-90980-img0001.jpg "ELISA: SARS-CoV-2 Spike Antibody (CR3022) [NBP2-90980] - Binding curve of SARS-CoV-2 Spike Antibody (CR3022) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 of various origin. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line), SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) and SARS Coronavirus Spike Glycoprotein (S1), His-Tag (HEK293 cells; blue line) (Native Antigen) at concentrations of 5 ug/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using SARS-CoV-2 Spike Antibody (CR3022). For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.")
![ELISA: SARS-CoV-2 Spike Antibody (CR3022) [NBP2-90980] - Binding curve of SARS-CoV-2 Spike Antibody (CR3022) to SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag and SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag from HEK293 cells. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag (blue line) or SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag (orange line) from HEK293 cells (Native Antigen) at concentrations of 5 ug/ml. A 3-fold serial dilution from 125 ng/ml was performed using SARS-CoV-2 Spike Antibody (CR3022). For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-CR3022-ELISA-NBP2-90980-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (CR3022) - Azide and BSA Free [NBP2-90980] - ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line) and SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) at concentrations of 5 ug/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using NBP2-90980; from 370 ng/ml for NBP3-11813 and from 10000 ng/ml for NBP3-11776. Human IgM and human IgA were HRP-conjugated and for the detection of human IgG1 a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.](http://images.novusbio.com/fullsize/nbp2-90980_human-monoclonal-sars-cov-2-spike-antibody-cr3022-7420252228562.png)
![ELISA: SARS-CoV-2 Spike Antibody [NBP3-11940] - Validation of SARS-Cov-2 Spike Antibody with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4C overnight. Detection Antibody: SARS-CoV-2 Spike antibody NBP3-11940, 0.2-1 ug/mL incubated at RT for 1 hr. Secondary Antibody: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. NBP3-11940 can detect spike S1 protein for all variants except omicron.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-ELISA-NBP3-11940-img0009.jpg "ELISA: SARS-CoV-2 Spike Antibody [NBP3-11940] - Validation of SARS-Cov-2 Spike Antibody with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4C overnight. Detection Antibody: SARS-CoV-2 Spike antibody NBP3-11940, 0.2-1 ug/mL incubated at RT for 1 hr. Secondary Antibody: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. NBP3-11940 can detect spike S1 protein for all variants except omicron.")
![Western Blot: SARS-CoV-2 Spike Antibody [NBP3-11940] - Western Blot Validation with SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein. Loading: 50 ng per lane of SARS-CoV-2 (COVID-19) Spike S1 recombinant protein. Antibodies: SARS-CoV-2 Spike, NBP3-11940, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Western-Blot-NBP3-11940-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Spike Antibody [NBP3-11940] - Immunofluorescence Validation of SARS-CoV-2 (COVID-19) Spike (cleavage site) in 293 Transfected Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Spike transfected 293cells labeling SARS-CoV-2 (COVID-19) Spike (cleavage site) with NBP3-11940 at 10 mg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11940-img0005.jpg)
![Western Blot: SARS-CoV-2 Spike Antibody [NBP3-11940] - Overexpression Validation in Spike Transfected 293 Cells. Loading: 10 ug per lane of 293 cell lysate. Antibodies: SARS-CoV-2 Spike, NBP3-11940 (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: WT 293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Western-Blot-NBP3-11940-img0006.jpg)
![Western Blot: SARS-CoV-2 Spike Antibody [NBP3-11940] - Western Blot Validation with SARS-CoV-2 (COVID-19) Spike Recombinant Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Spike RBD+SD1+SD2 recombinant protein. Antibodies: SARS-CoV-2 Spike, NBP3-11940, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL, Lane 2: 2 ug/mL and Lane 3: 4 ug/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Western-Blot-NBP3-11940-img0007.jpg)
![ELISA: SARS-CoV-2 Spike Antibody [NBP3-11940] - Detection of SARS-CoV-2 UK Variant Spike S1 Protein with Spike S1 Antibodies by Direct ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and UK variant (B.1.1.7), 1 ug/mL Incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike Antibody, NBP3-11940, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. Immunogen region of antibody NBP3-11940 includes site 681P that was mutated in B.1.1.7.. SARS-CoV-2 Spike antibody (NBP3-11940) has lower binding affinity for variant S1 protein as compared to WT S1 protein.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-ELISA-NBP3-11940-img0001.jpg)
![ELISA: SARS-CoV-2 Spike Antibody [NBP3-11940] - ELISA Validation of SARS-CoV-2 Spike Antibody Pair. A sandwich ELISA was performed using the anti-SARS-CoV-2 Spike antibodies NBP3-11940 (10 ug/mL ) as the capture antibody. Biotin-labled anti-SARS-COV-2 Spike S1 antibodies (1 ug/mL ) and streptavidin-HRP (0.1 ug/mL ) were used for detection. Detection range is from 0.8 ng to 200 ng (SARS-CoV-2 Spike S1 Protein).](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-ELISA-NBP3-11940-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody [NBP3-11940] - ELISA Validation with SARS-CoV-2 (COVID-19) Spike Recombinant Protein. Antibodies: SARS-CoV-2 Spike, NBP3-11940. A direct ELISA was performed using SARS-CoV-2 (COVID-19) Spike RBD+SD1+SD2 recombinant protein, as coating antigen and the anti-SARS-CoV-2 Spike as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 8 ng/mL to 1000ng/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-ELISA-NBP3-11940-img0003.jpg)
![ELISA: SARS-CoV-2 Spike Antibody [NBP3-11940] - ELISA Validation with SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein. Antibodies: SARS-CoV-2 Spike antibody, NBP3-11940 (1 ug/mL). A direct ELISA was performed using SARS-CoV-2 (COVID-19) Spike S1 recombinant protein as coating antigen and the anti-SARS-CoV-2 Spike antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-ELISA-NBP3-11940-img0004.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Sandwich ELISA detection of SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.75 variant, His tag using ELISA pairs below. Pair 1: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Pair 2: Capture: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Pair 3: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL) Please notice that NBP3-25671 and NBP3-25672 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014 and NBP3-25672. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249413310.jpg "ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Sandwich ELISA detection of SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.75 variant, His tag using ELISA pairs below. Pair 1: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Pair 2: Capture: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Pair 3: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL) Please notice that NBP3-25671 and NBP3-25672 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014 and NBP3-25672. Please contact us for custom HRP-conjugated antibody.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Indirect ELISA analysis was performed by coating the plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 Alpha variant; B.1.351 Beta variant; P.1 Gamma variant; B1.617.2 Delta variant; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) (28.78-0.45 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-212024940381.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Inhibition analysis of SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (104-13333 pM) using SARS-CoV-2 Neutralizing Antibody ELISA Kit (Omicron BA.1 / BA.2 / BA.4 / BA.5).](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-212024942542.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Sandwich ELISA detection of SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.75 variant, His tag using ELISA pairs below. Pair 1: Capture: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (HRP) (HL1014-01) (1 ug/mL) Pair 2: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (1 ug/mL)](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249351385.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BF.7 variant, His tag using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL) Please notice that NBP3-25672 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249355581.jpg)
![Functional (Inhibition): SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Inhibition analysis of immobilized recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) (coated at 2 ug/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (1000 ng/mL) binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (0.09-66.67 nM). Bound was detected by Goat Anti-Mouse IgG antibody (HRP) (1:10000).](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249374973.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., BA.4/BA.5 Omicron variant; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL) Please notice that NBP3-25672 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249425449.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.4/BA.5 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL) Please notice that NBP3-25672 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-212024941338.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BF.7 variant, His tag (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) or goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249415955.jpg)
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] detects SARS-CoV-2 (COVID-19) Spike RBD Omicron protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) Spike RBD Omicron stained by SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249374937.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Indirect ELISA analysis was performed by coating the plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., BA.2.12.1 Omicron variant; BA.4/BA.5 Omicron variant) (28.78-0.45 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249393569.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) or goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-212024940811.jpg)
![Immunohistochemistry: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Validation of SARS-CoV-2 Spike S1 in COVID-19 Patient Lung. Analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 Spike S1 antibody (0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 spike protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/nbp3-26918_rabbit-sars-cov-2-spike-s1-pab-bsa-free-242024161943.jpg "Immunohistochemistry: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Validation of SARS-CoV-2 Spike S1 in COVID-19 Patient Lung. Analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 Spike S1 antibody (0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 spike protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.")
![Western Blot: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Validation with SARS-CoV-2 Spike Recombinant Protein. Loading: 50 ng per lane of SARS-CoV-2 Spike S1 recombinant protein. Antibodies: SARS-CoV-2 Spike S1, NBP3-26918, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL.](http://images.novusbio.com/fullsize/nbp3-26918_rabbit-sars-cov-2-spike-s1-pab-bsa-free-242024161944.jpg)
![ELISA: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Validation with SARS-CoV-2 Spike Recombinant Protein. Antibodies: SARS-CoV-2 Spike S1 antibody, NBP3-26918. A direct ELISA was performed using SARS-CoV-2 (COVID-19) Spike S1 recombinant protein as coating antigen and the anti-SARS-CoV-2 Spike S1 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 8 ng/mL to 1000 ng/mL.](http://images.novusbio.com/fullsize/nbp3-26918_rabbit-sars-cov-2-spike-s1-pab-bsa-free-242024161940.jpg)
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Validation of SARS-CoV-2 Spike S1 in 293 Transfected Cells. Analysis of 4% paraformaldehyde-fixed Spike transfected 293 cells labeling SARS-CoV-2 Spike S1 with NBP3-26918 at 5 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp3-26918_rabbit-sars-cov-2-spike-s1-pab-bsa-free-242024161942.jpg)
![Western Blot: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Overexpression Validation in Spike Transfected 293 Cells. Loading: 10 ug per lane of 293 cell lysate. Antibodies: SARS-CoV-2 Spike S1, NBP3-26918 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: WT 293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells.](http://images.novusbio.com/fullsize/nbp3-26918_rabbit-sars-cov-2-spike-s1-pab-bsa-free-242024161941.jpg)
