| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB919-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF917-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3467-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | DDX0281B | |||
| HRP | DDX0281HRPO | |||
![Western Blot: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of MMP-12 on different lysates with Rabbit anti-MMP-12 antibody at 1/500 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Rat lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 40 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MMP-12-Antibody-SR03-23-Western-Blot-NBP2-67344-img0005.jpg "Western Blot: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of MMP-12 on different lysates with Rabbit anti-MMP-12 antibody at 1/500 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Rat lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 40 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of paraffin-embedded mouse lung tissue using anti-MMP12 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MMP-12-Antibody-SR03-23-Immunohistochemistry-Paraffin-NBP2-67344-img0007.jpg)
![Flow Cytometry: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of A549 cells with MMP12 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/MMP-12-Antibody-SR03-23-Flow-Cytometry-NBP2-67344-img0001.jpg)
![Western Blot: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of MMP12 on different cell lysates using anti-MMP12 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: Hela Lane 3: MCF-7 Lane 4: THP-1](http://images.novusbio.com/fullsize/MMP-12-Antibody-SR03-23-Western-Blot-NBP2-67344-img0004.jpg)
![Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of paraffin-embedded human breast carcinomna tissue using anti-MMP12 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MMP-12-Antibody-SR03-23-Immunohistochemistry-Paraffin-NBP2-67344-img0002.jpg)
![Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of paraffin-embedded mouse lung tissue using anti-MMP12 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MMP-12-Antibody-SR03-23-Immunohistochemistry-Paraffin-NBP2-67344-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP-12 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MMP-12-Antibody-SR03-23-Immunohistochemistry-Paraffin-NBP2-67344-img0006.jpg)
![Immunohistochemistry-Paraffin: Rabbit Monoclonal MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of MMP-12 antibody on human breast cancer tissue. Pretreatment: ER2 100C 20min, Blocking: 2.5% Normal horse serum 10min, Primary Ab: 1/200 60min, HRP for Rabbit 30min, DAB 5min, Hematoxylin counterstain. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-67344_rabbit-monoclonal-mmp-12-antibody-sr03-23-immunohistochemistry-paraffin-1102024134142..jpg)
![Western Blot: MMP-12 Antibody [NBP1-31225] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with MMP12 antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MMP-12-Antibody-Western-Blot-NBP1-31225-img0015.jpg "Western Blot: MMP-12 Antibody [NBP1-31225] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with MMP12 antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: MMP-12 Antibody [NBP1-31225] - Analysis of methanol-fixed H1299, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/MMP-12-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31225-img0007.jpg)
![Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - Imaging of Human breast cancer tissue. MMP12 is stained by antibody (green) and nucleus is stained with DAPI (blue). Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/MMP-12-Antibody-Immunohistochemistry-Paraffin-NBP1-31225-img0013.jpg)
![Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - DLD1 xenograft, using MMP12 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MMP-12-Antibody-Immunohistochemistry-Paraffin-NBP1-31225-img0008.jpg)
![Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - Human lung adenocarcinoma. MMP12 antibody [N3C1], Internal diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MMP-12-Antibody-Immunohistochemistry-Paraffin-NBP1-31225-img0012.jpg)
![ELISA: Human MMP-12 ELISA Kit (Colorimetric) [NBP3-06762] - Samples were spiked with high concentrations of Human MMP-12 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06762_human-mmp-12-elisa-kit-colorimetric-13220241685352.jpg "ELISA: Human MMP-12 ELISA Kit (Colorimetric) [NBP3-06762] - Samples were spiked with high concentrations of Human MMP-12 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human MMP-12 ELISA Kit (Colorimetric) [NBP3-06762] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06762_human-mmp-12-elisa-kit-colorimetric-43202495051.jpg)
![Immunohistochemistry: MMP-12 Antibody (706F9.01) [DDX0281P-100] - Staining of human tonsil frozen section.](http://images.novusbio.com/fullsize/MMP-12-Antibody-706F9-01-Immunohistochemistry-DDX0281P-100-img0004.jpg "Immunohistochemistry: MMP-12 Antibody (706F9.01) [DDX0281P-100] - Staining of human tonsil frozen section.")
![ELISA: MMP-12 Antibody (706F9.01) [DDX0281P-100] - ELISA with 706F9.01/701E4.03 anti-HME 1000 ng/ml1= control2= HME-transfected cells3= CD34++GMCSF+TNF- +IL44= patient serum of Langerhans histiocytosis](http://images.novusbio.com/fullsize/MMP-12-Antibody-706F9-01-ELISA-DDX0281P-100-img0006.jpg)
![Immunohistochemistry: MMP-12 Antibody (703D10) [DDX0283P-100] - Staining of human lung cancer frozen section.](http://images.novusbio.com/fullsize/MMP-12-Antibody-703D10-Immunohistochemistry-DDX0283P-100-img0005.jpg "Immunohistochemistry: MMP-12 Antibody (703D10) [DDX0283P-100] - Staining of human lung cancer frozen section.")
![Flow Cytometry: MMP-12 Antibody (703D10) [DDX0283P-100] - Intracellular staining of canine cells](http://images.novusbio.com/fullsize/MMP-12-Antibody-703D10-Flow-Cytometry-DDX0283P-100-img0003.jpg)
![ELISA: MMP-12 Antibody (703D10) [DDX0283P-100] - HumanHME-MMP12 ELISA assay](http://images.novusbio.com/fullsize/MMP-12-Antibody-703D10-ELISA-DDX0283P-100-img0006.jpg)
![Immunohistochemistry-Frozen: MMP-12 Antibody (603E6.22) [DDX0280P-100] - Analysis of HRP conjugate of DDX0280P-100. Staining of human lung cancer frozen section.](http://images.novusbio.com/fullsize/MMP-12-Antibody-603E6-22-Immunohistochemistry-Frozen-DDX0280P-100-img0007.jpg "Immunohistochemistry-Frozen: MMP-12 Antibody (603E6.22) [DDX0280P-100] - Analysis of HRP conjugate of DDX0280P-100. Staining of human lung cancer frozen section.")
![Immunohistochemistry: MMP-12 Antibody (603E6.22) [DDX0280P-100] - Analysis of human lung cancer frozen section with clone 603E6 (DX0280)](http://images.novusbio.com/fullsize/MMP-12-Antibody-603E6-22-Immunohistochemistry-DDX0280P-100-img0006.jpg)
![Immunohistochemistry: MMP-12 Antibody (603E6.22) [DDX0280P-100] - Human lung tissue sections](http://images.novusbio.com/fullsize/MMP-12-Antibody-603E6-22-Immunohistochemistry-DDX0280P-100-img0008.jpg)
![ELISA: Rat MMP-12 ELISA Kit (Colorimetric) [NBP3-06764] - Samples were spiked with high concentrations of Rat MMP-12 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06764_rat-mmp-12-elisa-kit-colorimetric-132202416254024.jpg "ELISA: Rat MMP-12 ELISA Kit (Colorimetric) [NBP3-06764] - Samples were spiked with high concentrations of Rat MMP-12 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat MMP-12 ELISA Kit (Colorimetric) [NBP3-06764] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06764_rat-mmp-12-elisa-kit-colorimetric-4320249133244.jpg)