CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Acetylcholinesterase/ACHE was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human Acetylcholinesterase/ACHE Monoclonal Antibody (Catalog # MAB7574) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7574-SP | |||
CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Acetylcholinesterase/ACHE was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Sheep Anti-Human Acetylcholinesterase/ACHE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7574) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7574-SP | |||
![Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Western-Blot-NBP1-31329-img0020.jpg "Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Mouse brain. AChE stained by AChE antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Immunohistochemistry-Paraffin-NBP1-31329-img0021.jpg)
![Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Western-Blot-NBP1-31329-img0009.jpg)
![Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Western-Blot-NBP1-31329-img0014.jpg)
![Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Western-Blot-NBP1-31329-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Paraformaldehyde-fixed HeLa, using AChE antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31329-img0008.jpg)
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Mouse colon. AChE antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Immunohistochemistry-Paraffin-NBP1-31329-img0010.jpg)
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Rat fore brain. AChE antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Immunohistochemistry-Paraffin-NBP1-31329-img0011.jpg)
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Rat brain. AChE antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Immunohistochemistry-Paraffin-NBP1-31329-img0012.jpg)
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Acetylcholinesterase/ACHE antibody detects Acetylcholinesterase/ACHE protein at nucleus by immunohistochemical analysis.<br>Sample: Paraffin-embedded mouse brain.<br>Acetylcholinesterase/ACHE stained by Acetylcholinesterase/ACHE antibody (NBP1-31329) diluted at 1:500.<br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31329_rabbit-polyclonal-acetylcholinesterase-ache-antibody-91020242091450.jpg)
![Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Acetylcholinesterase/ACHE antibody (NBP1-31329) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31329_rabbit-polyclonal-acetylcholinesterase-ache-antibody-910202420104428.jpg)
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Acetylcholinesterase/ACHE antibody detects Acetylcholinesterase/ACHE protein at nucleus by immunohistochemical analysis.<br>Sample: Paraffin-embedded rat brain.<br>Acetylcholinesterase/ACHE stained by Acetylcholinesterase/ACHE antibody (NBP1-31329) diluted at 1:500.<br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31329_rabbit-polyclonal-acetylcholinesterase-ache-antibody-91020242001048.jpg)
![Acetylcholinesterase/ACHE antibody detects Acetylcholinesterase/ACHE protein by immunofluorescent analysis.</br>Sample: DIV9 rat hippocampal neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: Acetylcholinesterase/ACHE stained by Acetylcholinesterase/ACHE antibody (NBP1-31329) diluted at 1:500.</br>Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.</br>Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31329_rabbit-polyclonal-acetylcholinesterase-ache-antibody-immunocytochemistry-immunofluorescence-1411202518443420.jpg)
![Acetylcholinesterase/ACHE antibody detects Acetylcholinesterase/ACHE protein expression by immunohistochemical analysis.<br>Sample: Frozen sectioned E13.5 Rat brain. <br>Green: Acetylcholinesterase/ACHE protein stained by Acetylcholinesterase/ACHE antibody (NBP1-31329) diluted at 1:250.<br>Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.<br>Blue: Fluoroshield with DAPI .<br>](http://images.novusbio.com/fullsize/nbp3-48812_rabbit-opsin-1-medium-wave-mab-hl3435-immunohistochemistry-frozen-14112025185548.jpg)
![Western Blot: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Staining (0.3ug/ml) of Jurkat (A) and (0.5ug/ml) HepG2 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Western-Blot-NB100-1519-img0006.jpg "Western Blot: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Staining (0.3ug/ml) of Jurkat (A) and (0.5ug/ml) HepG2 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear, membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1519-img0005.jpg)
![Flow Cytometry: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Flow-Cytometry-NB100-1519-img0004.jpg)
![Western Blot: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Acetylcholinesterase/ACHE antibody [HL1102] (NBP3-13720) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-HL1102-Western-Blot-NBP3-13720-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Acetylcholinesterase/ACHE antibody [HL1102] (NBP3-13720) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720] - Acetylcholinesterase/ACHE antibody [HL1102] detects Acetylcholinesterase/ACHE protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Acetylcholinesterase/ACHE stained by Acetylcholinesterase/ACHE antibody [HL1102] (NBP3-13720) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-HL1102-Immunohistochemistry-Paraffin-NBP3-13720-img0002.jpg)
![Western Blot: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Acetylcholinesterase/ACHE antibody [HL1102] (NBP3-13720) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-HL1102-Western-Blot-NBP3-13720-img0001.jpg)
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720] - Acetylcholinesterase/ACHE antibody [HL1102] detects Acetylcholinesterase/ACHE protein at cell membrane and cytoplasm by immunohistochemical analysis.</br>Sample: Paraffin-embedded cat brain.</br>Acetylcholinesterase/ACHE stained by Acetylcholinesterase/ACHE antibody [HL1102] (NBP3-13720) diluted at 1:100.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13720_rabbit-acetylcholinesterase-ache-mab-hl1102-91020242063394.jpg)
![Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720] - Acetylcholinesterase/ACHE antibody [HL1102] detects Acetylcholinesterase/ACHE protein at dendrite by immunofluorescent analysis.</br>Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: Acetylcholinesterase/ACHE stained by Acetylcholinesterase/ACHE antibody [HL1102] (NBP3-13720) diluted at 1:250.</br>Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.</br>Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13720_rabbit-acetylcholinesterase-ache-mab-hl1102-910202420115078.jpg)
![Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720] - Acetylcholinesterase/ACHE antibody [HL1102] detects Acetylcholinesterase/ACHE protein at endoplasmic reticulum by immunofluorescent analysis.</br>Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: Acetylcholinesterase/ACHE stained by Acetylcholinesterase/ACHE antibody [HL1102] (NBP3-13720) diluted at 1:500.</br>Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.</br>Blue: Fluoroshield with DAPI .</br>Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-13720_rabbit-acetylcholinesterase-ache-mab-hl1102-91020242011433.jpg)
![Western Blot: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Acetylcholinesterase/ACHE antibody [HL1102] (NBP3-13720) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-13720_rabbit-acetylcholinesterase-ache-mab-hl1102-91020242091470.jpg)
![Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody [HL1102] (NBP3-13720) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13720_rabbit-acetylcholinesterase-ache-mab-hl1102-western-blot-2611202518365911.jpg)
![N/A Acetylcholinesterase/ACHE [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Acetylcholinesterase_DY757405_ELISA_2508.jpg "N/A Acetylcholinesterase/ACHE [Biotin]")
![ELISA: Rat Acetylcholinesterase/ACHE ELISA Kit (Colorimetric) [NBP2-82443] - Samples were spiked with high concentrations of Rat Acetylcholinesterase/ACHE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82443_rat-acetylcholinesterase-ache-elisa-kit-colorimetric-13220241685357.jpg "ELISA: Rat Acetylcholinesterase/ACHE ELISA Kit (Colorimetric) [NBP2-82443] - Samples were spiked with high concentrations of Rat Acetylcholinesterase/ACHE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Acetylcholinesterase/ACHE ELISA Kit (Colorimetric) [NBP2-82443] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82443_rat-acetylcholinesterase-ache-elisa-kit-colorimetric-432024903591.jpg)
![ELISA: Mouse Acetylcholinesterase/ACHE ELISA Kit (Colorimetric) [NBP2-66392] - Samples were spiked with high concentrations of Mouse Acetylcholinesterase/ACHE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66392_mouse-acetylcholinesterase-ache-elisa-kit-colorimetric-132202416235766.jpg "ELISA: Mouse Acetylcholinesterase/ACHE ELISA Kit (Colorimetric) [NBP2-66392] - Samples were spiked with high concentrations of Mouse Acetylcholinesterase/ACHE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Acetylcholinesterase/ACHE ELISA Kit (Colorimetric) [NBP2-66392] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66392_mouse-acetylcholinesterase-ache-elisa-kit-colorimetric-4320249124857.jpg)
![ELISA: Human Acetylcholinesterase/ACHE ELISA Kit (Chemiluminescence) [NBP2-66390] - Samples were spiked with high concentrations of Human Acetylcholinesterase/ACHE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66390_human-acetylcholinesterase-ache-elisa-kit-chem-13220241626199.jpg "ELISA: Human Acetylcholinesterase/ACHE ELISA Kit (Chemiluminescence) [NBP2-66390] - Samples were spiked with high concentrations of Human Acetylcholinesterase/ACHE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Acetylcholinesterase/ACHE ELISA Kit (Chemiluminescence) [NBP2-66390] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66390_human-acetylcholinesterase-ache-elisa-kit-chem-432024973688.jpg)
![Acetylcholinesterase/ACHE Assay Kit (Fluorometric) [KA4132] - The standard curve is for the purpose of demonstration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Assay-Kit-Fluorometric-KA4132-img0001.jpg "Acetylcholinesterase/ACHE Assay Kit (Fluorometric) [KA4132] - The standard curve is for the purpose of demonstration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.")
