Products Related to Gene-ID: 4233

HAF008). A specific band was detected for Phospho-HGF R/ c-MET (Y1003) at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for Phospho-HGF R/ c-MET (Y1003) at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-HGF R/c-MET (Y1003) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4059), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-HGF R/ c-MET (Y1003) at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="HGF R/c-MET was detected in immersion fixed paraffin-embedded sections of human colon using 15 µg/mL Mouse Anti-Human HGF R/c-MET Monoclonal Antibody (Catalog # MAB3583) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF007). A specific band for HGF R/c-MET was detected at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF007). A specific band for HGF R/c-MET was detected at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 3 x 10⁵cells (lane 1), 2 x 10⁵cells (lane 2), and 1 x 10⁵cells (lane 3). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human HGF R/c-MET Monoclonal Antibody (Catalog # MAB5694) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for HGF R/c-MET was detected at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF018). A specific band was detected for HGF R/c-MET at approximately 150-200 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for HGF R/c-MET at approximately 150-200 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="HGFR/c-MET was detected in immersion fixed A431 human epithelial carcinoma cell line (Positive) & absent in MOLT‑4 human acute lymphoblastic leukemia cell line (Negative) using Rabbit Anti-Human HGFR/c-MET Monoclonal Antibody (Catalog # MAB11320) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

527-ME) was serially diluted and captured by Rat Anti-Mouse HGFR/c-MET Monoclonal Antibody (Catalog # MAB5271) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse HGFR/c-MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF527) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 527-ME) was serially diluted and captured by Rat Anti-Mouse HGFR/c-MET Monoclonal Antibody (Catalog # MAB5271) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse HGFR/c-MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF527) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse HGFR/c-MET Fc Chimera (Catalog # 527-ME) was serially diluted and captured by Rat Anti-Mouse HGFR/c-MET Monoclonal Antibody (Catalog # MAB5271) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse HGFR/c-MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF527) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />