294-HGN) with a ED50 of <0.400 μg/mL." alt="Measured by its binding ability in a functional ELISA. Recombinant Cynomolgus Monkey HGFR/c-MET His-tag Protein (Catalog # 11390-ME) binds to Recombinant Human HGF (NS0-expressed) Protein (294-HGN) with a ED50 of <0.400 μg/mL." class="big_thumb" />
11352-HG) is immobilized at 0.250 μg/mL (100 μL/well), Recombinant Cynomolgus HGFR/c-MET Fc Chimera Protein (Catalog # 11267-ME) binds with an ED50 of 2.50-25.0 ng/mL." alt="Measured by its binding ability in a functional ELISA. When Recombinant Human HGF (NS0-expressed) Protein (11352-HG) is immobilized at 0.250 μg/mL (100 μL/well), Recombinant Cynomolgus HGFR/c-MET Fc Chimera Protein (Catalog # 11267-ME) binds with an ED50 of 2.50-25.0 ng/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50172-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50171-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50170-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50169-0.025ml | |||
![N/A HGFR/c-MET [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_HGF_R_DY527_ELISA_2096.jpg "N/A HGFR/c-MET [Biotin]")
![N/A HGFR/c-MET [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_HGF_R_DY4140_ELISA_2012.jpg "N/A HGFR/c-MET [Biotin]")
![Western Blot: HGFR/c-MET Antibody (A9A4-R) [NBP3-32198] - Western blot analysis of HGFR/c-MET on different lysates with Mouse anti-HGFR/c-MET antibody (NBP3-32198) at 1/500 dilution.<br/><br/> Lane 1: A549 cell lysate<br/> Lane 2: HepG2 cell lysate<br/> Lane 3: HT-29 cell lysate<br/> Lane 4: Hela cell lysate<br/> Lane 5: LO2 cell lysate<br/> Lane 6: SK-Br-3 cell lysate (Negative)<br/><br/> Lysates/proteins at 30 ug/Lane.<br/><br/> Predicted band size: 156 kDa<br/> Observed band size: 145 kDa<br/><br/> Exposure time: 1 minute;<br/><br/> 6% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32198) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32198_mouse-hgfr-c-met-mab-a9a4-r-278202416331273.jpg "Western Blot: HGFR/c-MET Antibody (A9A4-R) [NBP3-32198] - Western blot analysis of HGFR/c-MET on different lysates with Mouse anti-HGFR/c-MET antibody (NBP3-32198) at 1/500 dilution.<br/><br/> Lane 1: A549 cell lysate<br/> Lane 2: HepG2 cell lysate<br/> Lane 3: HT-29 cell lysate<br/> Lane 4: Hela cell lysate<br/> Lane 5: LO2 cell lysate<br/> Lane 6: SK-Br-3 cell lysate (Negative)<br/><br/> Lysates/proteins at 30 ug/Lane.<br/><br/> Predicted band size: 156 kDa<br/> Observed band size: 145 kDa<br/><br/> Exposure time: 1 minute;<br/><br/> 6% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32198) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HGFR/c-MET Antibody (A9A4-R) [NBP3-32198] - Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Mouse anti-HGFR/c-MET antibody (NBP3-32198) at 1/400 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32198) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32198_mouse-hgfr-c-met-mab-a9a4-r-29820241416182.jpg)
![Western blot analysis on different lysates with NBP3-32198 at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: HT-29 cell lysate Lane 4: HeLa cell lysate Lane 5: LO2 cell lysate Lane 6: SK-Br-3 cell lysate (Negative) Lysates/proteins at 30 µg/Lane. Predicted band size: 156 kDa Observed band size: 145 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32198 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat anti-Mouse IgG [HRP] at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32198_-western-blot-639204590074335591.jpg)
![Adhesion Activation: HGF R/c-MET Antibody (8) [NBP2-50172] - Epitope position on molecule](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-8-Adhesion-Activation-NBP2-50172-img0001.jpg "Adhesion Activation: HGF R/c-MET Antibody (8) [NBP2-50172] - Epitope position on molecule")
![Immunoprecipitation: HGF R/c-MET Antibody (17) [NBP2-50171] - Immunoprecipitation of SNU-5 cell lysates using anti-c-Met [17]. Detection was performed by antibodies SC-10 and AF276.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-17-Immunoprecipitation-NBP2-50171-img0001.jpg "Immunoprecipitation: HGF R/c-MET Antibody (17) [NBP2-50171] - Immunoprecipitation of SNU-5 cell lysates using anti-c-Met [17]. Detection was performed by antibodies SC-10 and AF276.")
![Western Blot: Met (c-Met) Overexpression Lysate (Adult Normal) [NBL1-13017] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Met (c-Met).](http://images.novusbio.com/fullsize/Met-c-Met-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13017-img0002.jpg "Western Blot: Met (c-Met) Overexpression Lysate (Adult Normal) [NBL1-13017] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Met (c-Met).")
![Western Blot: HGF R/c-MET Antibody (12.1) [NBP2-50169] - Endogenous c-Met expression in a panel of human gastric cell lines. mAb12.1-HRP recognises 190kD pro-s-Met and 50kD mature c-Met alpha chain. mAb 12.1-HRP does not recognise the 145kD mature c-Met beta-chain.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-12-1-Western-Blot-NBP2-50169-img0001.jpg "Western Blot: HGF R/c-MET Antibody (12.1) [NBP2-50169] - Endogenous c-Met expression in a panel of human gastric cell lines. mAb12.1-HRP recognises 190kD pro-s-Met and 50kD mature c-Met alpha chain. mAb 12.1-HRP does not recognise the 145kD mature c-Met beta-chain.")
