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![Western Blot: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32811) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-288202412461627.jpg "Western Blot: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32811) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling Tau with Rabbit anti-Tau antibody (NBP3-32811) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32811, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-29820241492636.jpg)
![Immunohistochemistry: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32811) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-29820241420935.jpg)
![Immunohistochemistry-Paraffin: Tau Antibody [NBP2-49603] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/antibody/nbp2-49603_rabbit-polyclonal-tau-antibody-immunohistochemistry-paraffin-31202591128..png "Immunohistochemistry-Paraffin: Tau Antibody [NBP2-49603] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.")
![Immunohistochemistry-Paraffin: Tau Antibody [NBP2-49603] - Staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli and moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/antibody/nbp2-49603_rabbit-polyclonal-tau-antibody-immunohistochemistry-paraffin-31202591256..png)
![Immunohistochemistry-Paraffin: Tau Antibody [NBP2-49603] -Staining of human fallopian tube shows negative positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-49603_rabbit-polyclonal-tau-antibody-immunohistochemistry-paraffin-31202591414..png)
![Immunohistochemistry-Paraffin: Tau Antibody [NBP2-49603] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-49603_rabbit-polyclonal-tau-antibody-immunohistochemistry-paraffin-31202591526..png)
![Western Blot: Tau [p Thr231] Antibody (PSH01-05) [NBP3-32810] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32810) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32810) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32810_rabbit-tau-p-thr231-mab-psh01-05-288202412461665.jpg "Western Blot: Tau [p Thr231] Antibody (PSH01-05) [NBP3-32810] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32810) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32810) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Thr231] Antibody (PSH01-05) [NBP3-32810] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Tau antibody (NBP3-32810) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32810) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32810_rabbit-tau-p-thr231-mab-psh01-05-298202414161831.jpg)
![Western Blot: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32809) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32809_rabbit-tau-p-ser404-mab-je47-88-288202412461654.jpg "Western Blot: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32809) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32809) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32809_rabbit-tau-p-ser404-mab-je47-88-29820241492670.jpg)
![Western Blot: Tau [p Ser404] Antibody (JE66-82) [NBP3-32808] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32808) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32808) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32808_rabbit-tau-p-ser404-mab-je66-82-288202412461623.jpg "Western Blot: Tau [p Ser404] Antibody (JE66-82) [NBP3-32808] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32808) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32808) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Ser404] Antibody (JE66-82) [NBP3-32808] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tau antibody (NBP3-32808) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32808) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32808_rabbit-tau-p-ser404-mab-je66-82-298202414262664.jpg)
![Western Blot: Tau Antibody (1D5) - Pre-formed Fibrils [NBP2-79825] - Western Blot analysis of Human Breast Cancer Cell line and Mouse Brain showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 1D5 (NBP2-79825). Lane 1: MW Marker. Lane 2: Human T-47d (10ug). Lane 3: Mouse Brain (20ug). Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (NBP2-79825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.](http://images.novusbio.com/fullsize/Tau-Antibody-1D5-Pre-formed-Fibrils-Western-Blot-NBP2-79825-img0004.jpg "Western Blot: Tau Antibody (1D5) - Pre-formed Fibrils [NBP2-79825] - Western Blot analysis of Human Breast Cancer Cell line and Mouse Brain showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 1D5 (NBP2-79825). Lane 1: MW Marker. Lane 2: Human T-47d (10ug). Lane 3: Mouse Brain (20ug). Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (NBP2-79825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.")
![Dot Blot: Tau Antibody (1D5) - Pre-formed Fibrils [NBP2-79825] - Dot Blot analysis using Mouse Anti-Tau Monoclonal Antibody, Clone 1D5 (NBP2-79825). Tissue: Recombinant Protein. Species: Human. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (NBP2-79825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking.](http://images.novusbio.com/fullsize/Tau-Antibody-1D5-Pre-formed-Fibrils-Dot-Blot-NBP2-79825-img0005.jpg)
![Western Blot: Tau Antibody [NB100-82247] - Analysis of extracts from mouse brain tissue.](http://images.novusbio.com/fullsize/Tau-Antibody-Western-Blot-NB100-82247-img0005.jpg "Western Blot: Tau Antibody [NB100-82247] - Analysis of extracts from mouse brain tissue.")
![Immunohistochemistry-Paraffin: Tau Antibody [NB100-82247] - Analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer's Disease using NB100-82247](http://images.novusbio.com/fullsize/Tau-Antibody-Immunohistochemistry-NB100-82247-img0003.jpg)
![ELISA: Human Tau ELISA Kit (Colorimetric) [NBP2-62749] - Samples were spiked with high concentrations of Human Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-62749_human-tau-elisa-kit-colorimetric-13220241685316.jpg "ELISA: Human Tau ELISA Kit (Colorimetric) [NBP2-62749] - Samples were spiked with high concentrations of Human Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Tau ELISA Kit (Colorimetric) [NBP2-62749] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-62749_human-tau-elisa-kit-colorimetric-432024855036.jpg)
![ELISA: Rat Tau ELISA Kit (Colorimetric) [NBP2-81164] - Samples were spiked with high concentrations of Rat Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81164_rat-tau-elisa-kit-colorimetric-13220241617342.jpg "ELISA: Rat Tau ELISA Kit (Colorimetric) [NBP2-81164] - Samples were spiked with high concentrations of Rat Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Tau ELISA Kit (Colorimetric) [NBP2-81164] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81164_rat-tau-elisa-kit-colorimetric-4320248582587.jpg)
![Human Tau [p 739] ELISA Kit (Colorimetric) - Standard Curve Reference](http://images.novusbio.com/fullsize/Human-Tau-p-739-ELISA-Kit-Colorimetric-img0001.jpg "Human Tau [p 739] ELISA Kit (Colorimetric) - Standard Curve Reference")
![ELISA: Human Tau [p Ser739] ELISA Kit (Colorimetric) [NBP2-66711] - Samples were spiked with high concentrations of Human Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66711_human-tau-p-ser739-elisa-kit-colorimetric-132202416143078.jpg)
![ELISA: Human Tau [p Ser739] ELISA Kit (Colorimetric) [NBP2-66711] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66711_human-tau-p-ser739-elisa-kit-colorimetric-432024955011.jpg)
![Immunohistochemistry-Paraffin: Tau Antibody [NBP2-49607] - Staining in human cerebral cortex and liver tissues using anti-MAPT antibody. Corresponding MAPT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Tau-Antibody-Immunohistochemistry-Paraffin-NBP2-49607-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Tau Antibody [NBP2-49607] - Staining in human cerebral cortex and liver tissues using anti-MAPT antibody. Corresponding MAPT RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Tau Antibody [NBP2-49607] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Tau-Antibody-Immunohistochemistry-Paraffin-NBP2-49607-img0003.jpg)
![Immunohistochemistry-Paraffin: Tau Antibody [NBP2-49607] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Tau-Antibody-Immunohistochemistry-Paraffin-NBP2-49607-img0002.jpg)