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![Western Blot: Tau [p Ser198] Antibody [NBP3-12927] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Ser198) antibody (NBP3-12927) diluted at 1:5000.](http://images.novusbio.com/fullsize/Tau-[p-Ser198]-Antibody-Western-Blot-NBP3-12927-img0002.jpg "Western Blot: Tau [p Ser198] Antibody [NBP3-12927] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Ser198) antibody (NBP3-12927) diluted at 1:5000.")
![<b>Biological Strategies Validation. </b>Western Blot: Tau [p Ser198] Antibody [NBP3-12927] - Untreated (-) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Ser198) antibody (NBP3-12927) diluted at 1:500.](http://images.novusbio.com/fullsize/Tau-[p-Ser198]-Antibody-Western-Blot-NBP3-12927-img0001.jpg)
![Western Blot: Tau Antibody [NBP3-11044] - Western blot analysis of Mouse, Rat Brain showing detection of Multiple bands due to different isoforms/aggregation states Tau protein using Rabbit Anti-Tau Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse brain (15 ug). Lane 2: Rat brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple bands due to different isoforms/aggregation states. Other Band(s): 50 kDa, 65 kDa, 100 kDa.](http://images.novusbio.com/fullsize/Tau-Antibody-Western-Blot-NBP3-11044-img0001.jpg "Western Blot: Tau Antibody [NBP3-11044] - Western blot analysis of Mouse, Rat Brain showing detection of Multiple bands due to different isoforms/aggregation states Tau protein using Rabbit Anti-Tau Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse brain (15 ug). Lane 2: Rat brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple bands due to different isoforms/aggregation states. Other Band(s): 50 kDa, 65 kDa, 100 kDa.")
![Dot Blot: Tau Antibody [NBP3-11044] - Dot blot analysis using Rabbit Anti-Tau Polyclonal Antibody. Tissue: Recombinant Tau Monomers and Fibrils. Species: Human. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking.](http://images.novusbio.com/fullsize/Tau-Antibody-Dot-Blot-NBP3-11044-img0002.jpg)
![SDS-Page: Recombinant Human Tau 441 Active, Monomer, P301S Mutant Protein [NBP2-76795] - SDS-PAGE of ~67 kDa Human Tau Protein 2N4R P301S Monomer (NBP2-76795). Lane 1: MW ladder. Lane 2: Tau Protein Monomer (NBP2-76795)](http://images.novusbio.com/fullsize/Recombinant-Human-Tau-441-Active--Monomer--P301S-Mutant-Protein-SDS-Page-NBP2-76795-img0001.jpg "SDS-Page: Recombinant Human Tau 441 Active, Monomer, P301S Mutant Protein [NBP2-76795] - SDS-PAGE of ~67 kDa Human Tau Protein 2N4R P301S Monomer (NBP2-76795). Lane 1: MW ladder. Lane 2: Tau Protein Monomer (NBP2-76795)")
![In vitro assay: Recombinant Human Tau 441 Active, Monomer, P301S Mutant Protein [NBP2-76795] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in tau monomers (NBP2-76795). A greater increase in fluorescence is seen when 50 uM monomer (NBP2-76795) is combined with 10 nM PFFs , as the fibrils seed the formation of new fibrils from the pool of monomers. Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.](http://images.novusbio.com/fullsize/Recombinant-Human-Tau-441-Active--Monomer--P301S-Mutant-Protein-In-vitro-assay-NBP2-76795-img0002.jpg)
![ELISA: Rat Tau ELISA Kit (Colorimetric) [NBP2-81164] - Samples were spiked with high concentrations of Rat Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81164_rat-tau-elisa-kit-colorimetric-13220241617342.jpg "ELISA: Rat Tau ELISA Kit (Colorimetric) [NBP2-81164] - Samples were spiked with high concentrations of Rat Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Tau ELISA Kit (Colorimetric) [NBP2-81164] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81164_rat-tau-elisa-kit-colorimetric-4320248582587.jpg)
![Human Tau [p 739] ELISA Kit (Colorimetric) - Standard Curve Reference](http://images.novusbio.com/fullsize/Human-Tau-p-739-ELISA-Kit-Colorimetric-img0001.jpg "Human Tau [p 739] ELISA Kit (Colorimetric) - Standard Curve Reference")
![ELISA: Human Tau [p Ser739] ELISA Kit (Colorimetric) [NBP2-66711] - Samples were spiked with high concentrations of Human Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66711_human-tau-p-ser739-elisa-kit-colorimetric-132202416143078.jpg)
![ELISA: Human Tau [p Ser739] ELISA Kit (Colorimetric) [NBP2-66711] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66711_human-tau-p-ser739-elisa-kit-colorimetric-432024955011.jpg)
![ELISA: Human Tau ELISA Kit (Colorimetric) [NBP2-62749] - Samples were spiked with high concentrations of Human Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-62749_human-tau-elisa-kit-colorimetric-13220241685316.jpg "ELISA: Human Tau ELISA Kit (Colorimetric) [NBP2-62749] - Samples were spiked with high concentrations of Human Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Tau ELISA Kit (Colorimetric) [NBP2-62749] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-62749_human-tau-elisa-kit-colorimetric-432024855036.jpg)
![Western Blot: Rabbit Tau [p Thr217] mAb (HL1276) - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr217) antibody [HL1276] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45156_rabbit-tau-p-thr217-mab-hl1276-azide-and-bsa-free-western-blot-2942025165210..jpg "Western Blot: Rabbit Tau [p Thr217] mAb (HL1276) - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr217) antibody [HL1276] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![ELISA: Rabbit Tau [p Thr217] mAb (HL1276) [NBP3-45156] - Indirect ELISA analysis was performed by coating the plate with Tau peptide and Tau (p Thr217) peptide (1000-15.63 ng/mL). Coated peptides were probed with Tau (p Thr217) antibody [HL1276] (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-45156_rabbit-tau-p-thr217-mab-hl1276-azide-and-bsa-free-elisa-2942025164816..jpg)
![ELISA: Rat Tau [p Ser610] ELISA Kit (Colorimetric) [NBP2-76716] - Standard Curve Reference](http://images.novusbio.com/fullsize/Rat-Tau-p-Ser610-ELISA-Kit-Colorimetric-ELISA-NBP2-76716-img0001.jpg "ELISA: Rat Tau [p Ser610] ELISA Kit (Colorimetric) [NBP2-76716] - Standard Curve Reference")
![ELISA: Rat Tau [p Ser610] ELISA Kit (Colorimetric) [NBP2-76716] - Samples were spiked with high concentrations of Rat Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76716_rat-tau-p-ser610-elisa-kit-colorimetric-132202416235753.jpg)
![ELISA: Rat Tau [p Ser610] ELISA Kit (Colorimetric) [NBP2-76716] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76716_rat-tau-p-ser610-elisa-kit-colorimetric-432024903597.jpg)
![ELISA: Mouse Tau ELISA Kit (Colorimetric) [NBP2-81260] - Samples were spiked with high concentrations of Mouse Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81260_mouse-tau-elisa-kit-colorimetric-132202416232385.jpg "ELISA: Mouse Tau ELISA Kit (Colorimetric) [NBP2-81260] - Samples were spiked with high concentrations of Mouse Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Tau ELISA Kit (Colorimetric) [NBP2-81260] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81260_mouse-tau-elisa-kit-colorimetric-432024855058.jpg)
![Western Blot: Tau [p Ser202, p Ser199, p Ser198] Antibody [NBP3-16924] - Western blot analysis of extracts of NIH/3T3 cells, using Tau pAb (NBP3-16924) at 1:1000 dilution or Tau antibody.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 120s.](http://images.novusbio.com/fullsize/Tau-[p-Ser202-p-Ser199-p-Ser198]-Antibody-Western-Blot-NBP3-16924-img0001.jpg "Western Blot: Tau [p Ser202, p Ser199, p Ser198] Antibody [NBP3-16924] - Western blot analysis of extracts of NIH/3T3 cells, using Tau pAb (NBP3-16924) at 1:1000 dilution or Tau antibody.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 120s.")
![Immunohistochemistry: Tau [p Ser199] Antibody (23E8) [NBP3-26411] - Image of Tau [p Ser199] Antibody (23E8) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.10% DAB.](http://images.novusbio.com/fullsize/nbp3-26411_rabbit-tau-p-ser-199-mab-23e8-27220248471916.jpg "Immunohistochemistry: Tau [p Ser199] Antibody (23E8) [NBP3-26411] - Image of Tau [p Ser199] Antibody (23E8) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.10% DAB.")
![Flow Cytometry: Tau [p Ser199] Antibody (23E8) [NBP3-26411] - Overlay Peak curve showing SH-SY5Y cells stained with Tau [p Ser199] Antibody (23E8) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26411_rabbit-tau-p-ser-199-mab-23e8-282202414183638.jpg)