| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3494-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82236-0.025ml | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Atto488 | NBP3-14785-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB106291-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB10629-SP | |||
![Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-294202517153..jpg "<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-30420259378..jpg)
![Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Whole cell and rat tissue extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-304202593920..jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2497) - Sandwich ELISA detection of Tau peptide and Tau (p Thr181) peptide using antibodies as below.Capture: Tau (p Thr181) antibody [HL2498] (NBP3-45158) (5 ug/mL)Detection: HRP-conjugated Tau (p Thr181) antibody [HL2497] (NBP3-45157) (1 ug/mL)Please notice that NBP3-45157 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-45158.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-elisa-304202594949.jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2497) - Sandwich ELISA detection of Tau peptide and Tau (p Thr181) peptide using antibodies as below.Capture: Tau (phospho Thr181) antibody [HL2497] (NBP3-45157) (5 ug/mL)Detection: HRP-conjugated Tau (p Thr181) antibody [HL2498] (NBP3-45158) (1 ug/mL)Please notice that NBP3-45158 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-45157. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-elisa-304202512432..jpg)
![Immunohistochemistry: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Tau (p Thr181) antibody [HL2497] detects Tau (p Thr181) protein at cytoplasm by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).Sample: Paraffin-embedded mouse brain.Tau (p Thr181) stained by Tau (p Thr181) antibody [HL2497] (GTX638855) diluted at 1:100.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-immunohistochemistry-3042025122656..jpg)
![Western Blot: Tau [p Thr217] Antibody [NBP3-13481] - Untreated (-) and treated (+) Neuro2A whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr217) antibody (NBP3-13481) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Tau-[p-Thr217]-Antibody-Western-Blot-NBP3-13481-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Tau [p Thr217] Antibody [NBP3-13481] - Untreated (-) and treated (+) Neuro2A whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr217) antibody (NBP3-13481) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>ELISA: Tau [p Thr217] Antibody [NBP3-13481] - Indirect ELISA analysis performed by coating plate with recombinant partial Tau peptide and Tau (phospho Thr217) peptide (1000-0.003 ng/mL). Coated protein was probed with Tau (phospho Thr217) antibody (NBP3-13481) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (NBP2-19301) (1:10000) was used to detect bound primary antibody.](http://images.novusbio.com/fullsize/Tau-[p-Thr217]-Antibody-ELISA-NBP3-13481-img0002.jpg)
![IHC-P-Tau [p Thr217] Antibody-Rat brain. Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/antibody/nbp3-13481_rabbit-tau-p-thr-217-pab-immunohistochemistry-paraffin-89202384342..jpg)
![Tau (phospho Thr217) antibody detects Tau (phospho Thr217) protein at nucleus and cytoskeleton by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody (NBP3-13481) diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13481_rabbit-tau-p-thr-217-pab-immunocytochemistry-immunofluorescence-2611202518274612.jpg)
![Immunohistochemistry-Paraffin-NBP3-21454-Tau [p Ser396] Antibody (SN62-09)-Rat brain tissue using NBP3-21454. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp3-21454_rabbit-tau-p-ser396-mab-sn62-09-immunohistochemistry-paraffin-16820239811.jpg "Immunohistochemistry-Paraffin-NBP3-21454-Tau [p Ser396] Antibody (SN62-09)-Rat brain tissue using NBP3-21454. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Tau [p Ser396] Antibody (SN62-09) [NBP3-21454] - Mouse brain tissue using NBP3-21454. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp3-21454_rabbit-tau-p-ser396-mab-sn62-09-immunohistochemistry-paraffin-16820239936.jpg)
![Immunocytochemistry/ Immunofluorescence: Tau [p Ser396] Antibody (SN62-09) [NBP3-21454] - Immunocytochemical analysis of Tau [p Ser396] in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/nbp3-21454_rabbit-tau-p-ser396-mab-sn62-09-103202523464548.jpg)
![Immunocytochemistry/ Immunofluorescence: Tau [p Ser396] Antibody (SN62-09) [NBP3-21454] - Immunocytochemical analysis of Tau [p Ser396] in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/nbp3-21454_rabbit-tau-p-ser396-mab-sn62-09-103202523464554.jpg)
![Western Blot: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2498] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45158_rabbit-tau-p-thr181-mab-hl2498-azide-and-bsa-free-western-blot-152025102720.jpg "<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2498] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] - Untreated (–) and treated (+) Mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2498] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45158_rabbit-tau-p-thr181-mab-hl2498-azide-and-bsa-free-western-blot-152025103434..jpg)
![Immunohistochemistry: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] - Tau (p Thr181) antibody [HL2498] detects Tau (phospho Thr181) protein in the cytoplasm by immunohistochemical analysis in the presence of non phospho control peptide (left) but not in the presence of the antigenic phospho peptide (right).Sample: Paraffin-embedded mouse brain.Tau (p Thr181) stained by Tau (p Thr181) antibody [HL2498] diluted at 1:100.](http://images.novusbio.com/fullsize/nbp3-45158_rabbit-tau-p-thr181-mab-hl2498-azide-and-bsa-free-immunohistochemistry-152025103033..jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] - Sandwich ELISA detection of Tau peptide and Tau (phospho Thr181) peptide using antibodies as below.Capture: Tau (phospho Thr181) antibody [HL2498] (NBP3-45158) (5 ug/mL)Detection: HRP-conjugated Tau (phospho Thr181) antibody [HL2497] (NBP3-45157) (1 ug/mL)](http://images.novusbio.com/fullsize/nbp3-45158_rabbit-tau-p-thr181-mab-hl2498-azide-and-bsa-free-elisa-152025104331..jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2498) - Sandwich ELISA detection of Tau peptide and Tau (p Thr181) peptide using antibodies as below.Capture: Tau (p Thr181) antibody [HL2497] (NBP3-45157) (5 ug/mL)Detection: HRP-conjugated Tau (p Thr181) antibody [HL2498] (NBP3-45158) (1 ug/mL)](http://images.novusbio.com/fullsize/nbp3-45158_rabbit-tau-p-thr181-mab-hl2498-azide-and-bsa-free-elisa-152025105927..jpg)
![Western Blot: Tau Antibody [NB100-82236] - Analysis of extracts from mouse brain.](http://images.novusbio.com/fullsize/Tau-Antibody-Western-Blot-NB100-82236-img0002.jpg "Western Blot: Tau Antibody [NB100-82236] - Analysis of extracts from mouse brain.")
![SDS-Page: Recombinant Human Tau 441 Active, P301S Mutant, Pre-formed Fibrils Protein [NBP2-76794] - DS-PAGE of ~67 kDa Human Tau Protein 2N4R P301S Preformed Fibrils (NBP2-76794). Lane 1: MW Ladder. Lane 2: Tau Protein Preformed Fibrils (NBP2-76794)](http://images.novusbio.com/fullsize/Recombinant-Human-Tau-441-Active--P301S-Mutant--Pre-formed-Fibrils-Protein-SDS-Page-NBP2-76794-img0001.jpg "SDS-Page: Recombinant Human Tau 441 Active, P301S Mutant, Pre-formed Fibrils Protein [NBP2-76794] - DS-PAGE of ~67 kDa Human Tau Protein 2N4R P301S Preformed Fibrils (NBP2-76794). Lane 1: MW Ladder. Lane 2: Tau Protein Preformed Fibrils (NBP2-76794)")
![In vitro assay: Recombinant Human Tau 441 Active, P301S Mutant, Pre-formed Fibrils Protein [NBP2-76794] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) when tau Pre-formed Fibrils (NBP2-76794) are combined with tau monomers . The Pre-formed Fibrils seed the formation of new fibrils from a pool of monomers. Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.](http://images.novusbio.com/fullsize/Recombinant-Human-Tau-441-Active--P301S-Mutant--Pre-formed-Fibrils-Protein-In-vitro-assay-NBP2-76794-img0002.jpg)
![Electron Microscopy: Recombinant Human Tau 441 Active, P301S Mutant, Pre-formed Fibrils Protein [NBP2-76794] - TEM of recombinant Tau 441441 (2N4R), P301S mutant Pre-formed Fibrils (PFFs). Fibrils were sonicated and stained with uranyl acetate.](http://images.novusbio.com/fullsize/Recombinant Human Tau 441 Active- P301S Mutant- Pre-formed Fibrils Protein-Electron Microscopy-NBP2-76794-img0004.jpg)
![Electron Microscopy: Recombinant Human Tau 441 Active, P301S Mutant, Pre-formed Fibrils Protein [NBP2-76794] - TEM of recombinant Tau 441441 (2N4R), P301S mutant Pre-formed Fibrils (PFFs) at 150kx magnification. HV = 80kV. Fibrils were sonicated and stained with uranyl acetate.](http://images.novusbio.com/fullsize/Recombinant-Human-Tau-441-Active--P301S-Mutant--Pre-formed-Fibrils-Protein-Electron-Microscopy-NBP2-76794-img0003.jpg)
![Simple Western: Tau [p Ser262] Antibody [NB100-82242] - Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice. Image courtesy of Dr. Brandi Wasek-Patterson at Baylor Research Institute, Institute of Metabolic Disease.](http://images.novusbio.com/fullsize/Tau-[p-Ser262]-Antibody-Simple-Western-NB100-82242-img0004.jpg "Simple Western: Tau [p Ser262] Antibody [NB100-82242] - Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice. Image courtesy of Dr. Brandi Wasek-Patterson at Baylor Research Institute, Institute of Metabolic Disease.")
![Western Blot: Tau [p Ser262] Antibody [NB100-82242] - Western blot analysis of lysates from HeLa cells, using NB100-82242. The lane on the left is blocked with the phospho peptide.](http://images.novusbio.com/fullsize/nb100-82242_rabbit-polyclonal-tau-p-ser262-antibody-234202415453510.jpg)
![Western Blot: Rabbit Tau mAb (HL2982) [NBP3-45159] - Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2982] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45159_rabbit-tau-mab-hl2982-azide-and-bsa-free-western-blot-152025112952..jpg "Western Blot: Rabbit Tau mAb (HL2982) [NBP3-45159] - Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2982] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Rabbit Tau mAb (HL2982) [NBP3-45159] - Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2982] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45159_rabbit-tau-mab-hl2982-azide-and-bsa-free-western-blot-15202511328..jpg)
![Western Blot: Rabbit Tau mAb (HL2982) - Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2982] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45159_rabbit-tau-mab-hl2982-azide-and-bsa-free-western-blot-15202511382..jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2982) [NBP3-45159] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2982] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based.](http://images.novusbio.com/fullsize/nbp3-45159_rabbit-tau-mab-hl2982-azide-and-bsa-free-western-blot-152025115512..jpg)
![Western Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Western Blot analysis of Human iPSC-derived cortical neurons showing detection of Tau protein using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Lane 1: MW ladder. Lane 2: Control (non-disease) line. Lane 2: Ex10+16 tau mutant sample. Lane 3: P301L tau mutant sample. Load: 50ug. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. pSer202/pThr205 was detected using NBP3-18265. Total tau was detected using mouse anti-tau antibody (clone HT7). The bar graph on the right shows quantification of pSer202/pThr205 compared to total tau in each sample.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Western-Blot-NBP3-18265-img0002.jpg "Western Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Western Blot analysis of Human iPSC-derived cortical neurons showing detection of Tau protein using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Lane 1: MW ladder. Lane 2: Control (non-disease) line. Lane 2: Ex10+16 tau mutant sample. Lane 3: P301L tau mutant sample. Load: 50ug. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. pSer202/pThr205 was detected using NBP3-18265. Total tau was detected using mouse anti-tau antibody (clone HT7). The bar graph on the right shows quantification of pSer202/pThr205 compared to total tau in each sample.")
![Immunocytochemistry/Immunofluorescence: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Tissue: iPSC-derived cortical excitatory neurons. Species: Human. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. Secondary Antibody: Donkey anti-rabbit: Alexa Fluor 488 at 1:1000. Counterstain: DAPI. A) iPSC-derived neurons from non-demented control (NDC). B) iPSC-derived neurons from subject with P301L MAPT mutation. Images acquired using an automated Opera Phoenix system. Each field of view is a max projection from 10 planes of 1 um stacks.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Immunocytochemistry-Immunofluorescence-NBP3-18265-img0001.jpg)
![Dot Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Dot Blot analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Species: E. Coli, Baculovirus. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500. Secondary Antibody: Goat anti-rabbit IgG:HRP.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Dot-Blot-NBP3-18265-img0003.jpg)