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![Western Blot: SMAD1/5/9 Antibody (RM487)[NBP3-18834]Western Blot of mouse liver tissue lysate, using Anti-Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) rabbit monoclonal antibody (clone RM487) at a 1:1000 dilution. The phospho-specificity of RM487 was verified by peptide blocking using a control nonphospho-peptide or phosphor-peptide targeting residue Ser463/465 of Smad1 and Smad5 and residue Ser465/467 of Smad9.](http://images.novusbio.com/fullsize/nbp3-18834_rabbit-smad1-5-9-mab-rm487-161120231301814.png "Western Blot: SMAD1/5/9 Antibody (RM487)[NBP3-18834]Western Blot of mouse liver tissue lysate, using Anti-Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) rabbit monoclonal antibody (clone RM487) at a 1:1000 dilution. The phospho-specificity of RM487 was verified by peptide blocking using a control nonphospho-peptide or phosphor-peptide targeting residue Ser463/465 of Smad1 and Smad5 and residue Ser465/467 of Smad9.")
![ELISA: Rat Smad1 ELISA Kit (Colorimetric) [NBP2-76617] - Samples were spiked with high concentrations of Rat Smad1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76617_rat-smad1-elisa-kit-colorimetric-132202416215373.jpg "ELISA: Rat Smad1 ELISA Kit (Colorimetric) [NBP2-76617] - Samples were spiked with high concentrations of Rat Smad1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Smad1 ELISA Kit (Colorimetric) [NBP2-76617] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76617_rat-smad1-elisa-kit-colorimetric-432024924086.jpg)
![ELISA: Human Smad1 ELISA Kit (Colorimetric) [NBP2-76618] - Samples were spiked with high concentrations of Human Smad1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76618_human-smad1-elisa-kit-colorimetric-132202416121380.jpg "ELISA: Human Smad1 ELISA Kit (Colorimetric) [NBP2-76618] - Samples were spiked with high concentrations of Human Smad1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Smad1 ELISA Kit (Colorimetric) [NBP2-76618] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76618_human-smad1-elisa-kit-colorimetric-4320249151616.jpg)
![ELISA: Mouse Smad1 ELISA Kit (Colorimetric) [NBP2-76616] - Samples were spiked with high concentrations of Mouse Smad1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76616_mouse-smad1-elisa-kit-colorimetric-132202416211371.jpg "ELISA: Mouse Smad1 ELISA Kit (Colorimetric) [NBP2-76616] - Samples were spiked with high concentrations of Mouse Smad1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Smad1 ELISA Kit (Colorimetric) [NBP2-76616] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76616_mouse-smad1-elisa-kit-colorimetric-4320249141688.jpg)
![Western Blot: Smad1 [p Ser206] Antibody [NBP1-77860] - This antibody was used at a 1:500 dilution in 1% BLOTTO and reacted for 1 hour at room temperature. Anti-beta actin staining was used as a loading control. The membrane was washed and reacted with a 1:3,000 dilution HRP-conjugated a-Rabbit IgG for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX](http://images.novusbio.com/fullsize/Smad1-[p-Ser206]-Antibody-Western-Blot-NBP1-77860-img0004.jpg "Western Blot: Smad1 [p Ser206] Antibody [NBP1-77860] - This antibody was used at a 1:500 dilution in 1% BLOTTO and reacted for 1 hour at room temperature. Anti-beta actin staining was used as a loading control. The membrane was washed and reacted with a 1:3,000 dilution HRP-conjugated a-Rabbit IgG for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX")
![Western Blot: Smad1 [p Ser206] Antibody [NBP1-77858] - Shows detection of endogenous phosphorylated SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) derived cell lines treated with PBS, BMP2 (5 ug/ml), EGF and reacted for 1 hour at room temperature. Primary antibody was pre-incubated before reaction with blot as follows: top row - with PBS, middle row - with the immunizing phosphorylated peptide and bottom row - with control or non-phosphorylated peptide. The membrane was washed and reacted with a 1:3,000 dilution HRP-conjugated a-Rabbit IgG for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX.](http://images.novusbio.com/fullsize/Smad1-[p-Ser206]-Antibody-Western-Blot-NBP1-77858-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: Smad1 [p Ser206] Antibody [NBP1-77858] - Shows detection of endogenous phosphorylated SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) derived cell lines treated with PBS, BMP2 (5 ug/ml), EGF and reacted for 1 hour at room temperature. Primary antibody was pre-incubated before reaction with blot as follows: top row - with PBS, middle row - with the immunizing phosphorylated peptide and bottom row - with control or non-phosphorylated peptide. The membrane was washed and reacted with a 1:3,000 dilution HRP-conjugated a-Rabbit IgG for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX.")
![Western Blot: Smad1 [p Ser463, p Ser465] Antibody (S07-4A3) [NBP3-15092] - Western blot detection of Smad1 [p Ser463, p Ser465] in A549, U2OS, C6 cell lysates using Smad1 [p Ser463, p Ser465] Rabbit mAb (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 52kDa.](http://images.novusbio.com/fullsize/Smad1-p-Ser463-p-Ser465-Antibody-S07-4A3-Western-Blot-NBP3-15092-img0001.jpg "Western Blot: Smad1 [p Ser463, p Ser465] Antibody (S07-4A3) [NBP3-15092] - Western blot detection of Smad1 [p Ser463, p Ser465] in A549, U2OS, C6 cell lysates using Smad1 [p Ser463, p Ser465] Rabbit mAb (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 52kDa.")
![Western Blot: SMAD1 Overexpression Lysate (Adult Normal) [NBL1-16219] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SMAD1.](http://images.novusbio.com/fullsize/SMAD1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16219-img0002.jpg "Western Blot: SMAD1 Overexpression Lysate (Adult Normal) [NBL1-16219] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SMAD1.")
![Western Blot: SMAD1 Overexpression Lysate (Adult Normal) [NBL1-16218] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SMAD1.](http://images.novusbio.com/fullsize/SMAD1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16218-img0002.jpg "Western Blot: SMAD1 Overexpression Lysate (Adult Normal) [NBL1-16218] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SMAD1.")