| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80582 | |||
| BSA Free | NB100-124SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Purified | NBP3-25350SS | |||
| Purified | NBP3-25350 | |||
| BSA Free | NB100-110SS | |||
HAF109). A specific band was detected for ARNT/HIF-1 beta at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ARNT/HIF-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5630) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ARNT/HIF-1 beta at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5630-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54663-25ul | |||
HAF018). A specific band was detected for ARNT/HIF-1 beta at approximately 90-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ARNT/HIF-1 beta Monoclonal Antibody (Catalog # MAB5630) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ARNT/HIF-1 beta at approximately 90-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5630-SP | |||
![Immunohistochemistry: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Staining of human glioblastoma multi-forme utilizing ARNT/HIF-1 beta antibody (H1beta234) [NB100-124].](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-H1beta234-Immunohistochemistry-NB100-124-img0005.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Analysis of HIF-1 beta in HeLa nuclear extract using ARNT/HIF-1 beta antibody (H1beta234) [NB100-124]. Theoretical molecular weight 86.6 kDa. Observed molecular weight ~85 kDa.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-H1beta234-Western-Blot-NB100-124-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with ARNT/HIF-1 beta antibody (H1beta234) [NB100-124] at 5 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-H1beta234-Immunocytochemistry-Immunofluorescence-NB100-124-img0007.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) - BSA Free [NB100-124] - PGE1 induces HIF-1 alpha protein accumulation in vascular-derived cells.Human aortic smooth muscle cells (HASMCs) (A & B), human umbilical vein endothelial cells (HUVECs) (C & D), & HEK293 cells (G) were exposed to 1 or 10 µM PGE1 under 20% O2 or 1% O2 conditions for 4 h. After treatment, cells were harvested & whole-cell lysates were subjected to immunoblot assay for HIF-1 alpha , HIF-1 beta , & beta -actin protein expression. Experiments were repeated thrice (A, C & G). Representative immunoblots are shown (A & C). Band intensities were analyzed densitometrically (B & D). Fold induction relative to lane 1 was plotted as mean ± S.D. ∗P < 0.05 compared with the control. HASMCs (E) & HUVECs (F) were exposed to 1 µM PGE1 for the indicated times under 20% O2 & were harvested for immunoblot assay for HIF-1 alpha protein. Experiments were repeated twice. Representative immunoblots are shown. (H) HASMCs & HUVECs were exposed to 10 µM PGE1 for 4 h under 20% O2 & were harvested for immunoblot assay for HIF-2 alpha protein. Experiments were repeated twice. Representative immunoblots are shown. I. HASMCs were exposed to 1 µM PGE1, lipo-PGE1 & PGE1-alfadex under 20% O2 conditions for 4 h. After treatment, cells were harvested & whole-cell lysates were subjected to immunoblot assay for HIF-1 alpha . Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24349900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-124_mouse-monoclonal-arnt-hif-1-beta-antibody-h1beta234-310202415532055.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) - BSA Free [NB100-124] - Effect of PGE1 on stability & synthesis of HIF-1 alpha .(A) Human aortic smooth muscle cells (HASMCs) & human umbilical vein endothelial cells (HUVECs) were exposed to 1 µM PGE1 or 100 µM DFX or incubated for 4 h, & CHX was added to a final concentration of 100 µM. The cells were incubated for 0 to 30 min, & whole-cell lysates were subjected to immunoblot assay using anti-HIF-1 alpha or -beta antibodies. (B) Serum-starved HASMCs were pretreated with no drug & 1 µM PGE1 for 30 min in Met-free medium. [35S]Met-Cys was added, & the cells were incubated for 60 min prior to preparation of cell lysates. Aliquots of 1 mg of the lysates were subjected to immunoprecipitation with anti-HIF-1 alpha antibody, separated by SDS-PAGE & exposed. Aliquots of 50 µg of the same lysate were separately subjected to immunoblotting analysis with anti-beta -actin antibody. (C) HASMCs & HUVECs were exposed to vehicle or 1 µM PGE1 for 4 h in the presence of 10 µM LY294002 (LY), 50 µM PD98059 (PD), or 5 µM GF109203X (GF). The cells were harvested & the whole-cell lysates were subjected to immunoblot assay for HIF-1 alpha & beta -actin protein expression. Experiments were repeated at least twice. Representative immunoblots are shown. (D) HASMCs were exposed to vehicle or 1 µM PGE1 for 12 h in the presence of 10 µM LY294002 (LY), 50 µM PD98059 (PD), or 5 µM GF109203X (GF). Cells were harvested & subjected to semi-quantitative RT-PCR for VEGF & GLUT1. Experiments were repeated three times. Fold induction relative to that under 20% O2 without PGE1 treatment was plotted. ∗P < 0.05 compared with the control (20%, PGE1 treatment without any kinase inhibitors). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24349900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-124_mouse-monoclonal-arnt-hif-1-beta-antibody-h1beta234-31020241555235.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) - BSA Free [NB100-124] - Effect of PGE1 on stability & synthesis of HIF-1 alpha .(A) Human aortic smooth muscle cells (HASMCs) & human umbilical vein endothelial cells (HUVECs) were exposed to 1 µM PGE1 or 100 µM DFX or incubated for 4 h, & CHX was added to a final concentration of 100 µM. The cells were incubated for 0 to 30 min, & whole-cell lysates were subjected to immunoblot assay using anti-HIF-1 alpha or -beta antibodies. (B) Serum-starved HASMCs were pretreated with no drug & 1 µM PGE1 for 30 min in Met-free medium. [35S]Met-Cys was added, & the cells were incubated for 60 min prior to preparation of cell lysates. Aliquots of 1 mg of the lysates were subjected to immunoprecipitation with anti-HIF-1 alpha antibody, separated by SDS-PAGE & exposed. Aliquots of 50 µg of the same lysate were separately subjected to immunoblotting analysis with anti-beta -actin antibody. (C) HASMCs & HUVECs were exposed to vehicle or 1 µM PGE1 for 4 h in the presence of 10 µM LY294002 (LY), 50 µM PD98059 (PD), or 5 µM GF109203X (GF). The cells were harvested & the whole-cell lysates were subjected to immunoblot assay for HIF-1 alpha & beta -actin protein expression. Experiments were repeated at least twice. Representative immunoblots are shown. (D) HASMCs were exposed to vehicle or 1 µM PGE1 for 12 h in the presence of 10 µM LY294002 (LY), 50 µM PD98059 (PD), or 5 µM GF109203X (GF). Cells were harvested & subjected to semi-quantitative RT-PCR for VEGF & GLUT1. Experiments were repeated three times. Fold induction relative to that under 20% O2 without PGE1 treatment was plotted. ∗P < 0.05 compared with the control (20%, PGE1 treatment without any kinase inhibitors). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24349900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-124_mouse-monoclonal-arnt-hif-1-beta-antibody-h1beta234-310202415541969.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) - BSA Free [NB100-124] - PGE1 induces HIF-1 alpha protein accumulation in vascular-derived cells.Human aortic smooth muscle cells (HASMCs) (A & B), human umbilical vein endothelial cells (HUVECs) (C & D), & HEK293 cells (G) were exposed to 1 or 10 µM PGE1 under 20% O2 or 1% O2 conditions for 4 h. After treatment, cells were harvested & whole-cell lysates were subjected to immunoblot assay for HIF-1 alpha , HIF-1 beta , & beta -actin protein expression. Experiments were repeated thrice (A, C & G). Representative immunoblots are shown (A & C). Band intensities were analyzed densitometrically (B & D). Fold induction relative to lane 1 was plotted as mean ± S.D. ∗P < 0.05 compared with the control. HASMCs (E) & HUVECs (F) were exposed to 1 µM PGE1 for the indicated times under 20% O2 & were harvested for immunoblot assay for HIF-1 alpha protein. Experiments were repeated twice. Representative immunoblots are shown. (H) HASMCs & HUVECs were exposed to 10 µM PGE1 for 4 h under 20% O2 & were harvested for immunoblot assay for HIF-2 alpha protein. Experiments were repeated twice. Representative immunoblots are shown. I. HASMCs were exposed to 1 µM PGE1, lipo-PGE1 & PGE1-alfadex under 20% O2 conditions for 4 h. After treatment, cells were harvested & whole-cell lysates were subjected to immunoblot assay for HIF-1 alpha . Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24349900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-124_mouse-monoclonal-arnt-hif-1-beta-antibody-h1beta234-310202415541928.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) - BSA Free [NB100-124] - Differential involvement of EP receptors in PGE1-induced HIF-1 alpha protein accumulation.(A) Expression of EP1, EP2, EP3, & EP4 receptors in human aortic smooth muscle cells (HASMCs), human umbilical vein endothelial cells (HUVECs), & cells of the neuroblastoma cell line SH-SY5Y. HASMCs, HUVECs, & SH-SY5Y cells were cultured under 20% O2 & harvested for semi-quantitative RT-PCR for EP1-4 receptors. Experiments were repeated at least three times in triplicate. Fold induction relative to expression in SH-SY5Y cells was plotted as mean ± S.D. HASMCs & HUVECs were exposed to 1 µM of EP-receptor-specific agonists (ONO-DI-004 for EP1, ONO-AE1-259-01 for EP2, ONO-AE-248 for EP3, & ONO-AE1-329 for EP4) for 4 h (B). HASMCs & HUVECs were exposed to 1 µM PGE1 with or without 1 µM EP-receptor-specific antagonists (ONO-8713 against EP1, ONO-AE3-240 against EP3, & ONO-AE2-227 against EP4) for 4 h (C). The cells were harvested & the whole-cell lysates were subjected to immunoblot assay for HIF-1 alpha & beta -actin protein expression. Experiments were repeated twice. Representative immunoblots are shown. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24349900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-124_mouse-monoclonal-arnt-hif-1-beta-antibody-h1beta234-310202415533849.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) - BSA Free [NB100-124] - HIF was not the only factor stabilizing activated EGFR in VHL-deficient ccRCC cells.A. Western blot analysis of 786-VHL & 786-mock cells stably expressing shRNA constructs. For HIF2 alpha & HIF1 beta analysis, nuclear extracts were generated & analyzed. Anti-HA blot detected HA-VHL. B. 786-VHL cells expressing SCR (control), HIF2a-566 & HIF2a-1631 (shRNA constructs against HIF2 alpha ) were treated with EGF & analyzed as described in Fig. 1A. C. 786-mock cells expressing SCR (control sequence), HIF2a-566 & HIF2a-1631 (shRNA constructs against HIF2 alpha ) were treated with EGF & analyzed as described in Fig. 2B. D. The EGFR signals in Fig. 2B & 2C were normalized over Vinculin via densitometry & plotted over time. Means & SDs of three separate experiments were shown. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0023936), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-124_mouse-monoclonal-arnt-hif-1-beta-antibody-h1beta234-310202415535160.jpg)
![Immunocytochemistry/ Immunofluorescence: ARNT/HIF-1 beta Antibody (H1beta234) - BSA Free [NB100-124] - Co-localization of HIF-2 alpha with HIF-1 beta & RNAPII. (a) Immunofluorescence detecting indicated proteins using antibodies labelled with Alexa Fluor 555 (red, pseudocolour) & Alexa Fluor 488 (green, pseudocolour). ‘Merge’ is the red image superimposed onto the green image of the co-stained nuclear proteins. ‘Coloc’ is the co-localization channel calculated using ImageJ plugin Co-localization Threshold. White indicates pixels where both red & green signal is found (i.e. co-localization). ‘Overlay’ is the co-localization image superimposed onto the merged image. Inlay is the magnified region (white square). White arrows highlight regions of co-localization. Scale bar, 5 μm. Abbreviations: RNAPII, RNA Polymerase II phospho-serine 5. (b) Immunofluorescence images were analysed using ImageJ plugin Co-localization Threshold with use of the Costes et al. [31] method to automatically create a threshold prior to calculating the Mander's coefficient for both proteins. The results are given as, for example, the percentage of protein A (HIF-2 alpha ) that co-localized with protein B (HIF-1 beta or RNAPII) & vice versa. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27655733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-124_mouse-monoclonal-arnt-hif-1-beta-antibody-h1beta234-310202415541927.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) - BSA Free [NB100-124] - Differential involvement of EP receptors in PGE1-induced HIF-1 alpha protein accumulation.(A) Expression of EP1, EP2, EP3, & EP4 receptors in human aortic smooth muscle cells (HASMCs), human umbilical vein endothelial cells (HUVECs), & cells of the neuroblastoma cell line SH-SY5Y. HASMCs, HUVECs, & SH-SY5Y cells were cultured under 20% O2 & harvested for semi-quantitative RT-PCR for EP1-4 receptors. Experiments were repeated at least three times in triplicate. Fold induction relative to expression in SH-SY5Y cells was plotted as mean ± S.D. HASMCs & HUVECs were exposed to 1 µM of EP-receptor-specific agonists (ONO-DI-004 for EP1, ONO-AE1-259-01 for EP2, ONO-AE-248 for EP3, & ONO-AE1-329 for EP4) for 4 h (B). HASMCs & HUVECs were exposed to 1 µM PGE1 with or without 1 µM EP-receptor-specific antagonists (ONO-8713 against EP1, ONO-AE3-240 against EP3, & ONO-AE2-227 against EP4) for 4 h (C). The cells were harvested & the whole-cell lysates were subjected to immunoblot assay for HIF-1 alpha & beta -actin protein expression. Experiments were repeated twice. Representative immunoblots are shown. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24349900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-124_mouse-monoclonal-arnt-hif-1-beta-antibody-h1beta234-3102024165732.jpg)
![Immunocytochemistry/ Immunofluorescence: ARNT/HIF-1 beta Antibody (H1beta234) - BSA Free [NB100-124] - Sub-nuclear localization of HIF-1 alpha & HIF-2 alpha . (a) HeLa cells ectopically expressing HIF-1 alpha & HIF-2 alpha EGFP fusions compared with endogenous HIF-1 alpha & HIF-2 alpha labelled using immunostaining. Images of HIF-1 alpha were taken following DMOG treatment (6 h; 0.5 mM). Scale bar, 5 μm. (b) HeLa cells transiently transfected with plasmids encoding (i) clover-HIF-2 alpha (green, pseudocolour), (ii) dsRED-HIF-2 alpha (red, pseudocolour), (iii) HIF-2 alpha -venus (yellow pseudocolour) & (iv) Halotag-HIF-2 alpha (green, pseudocolour). The cells expressing Halotag-HIF-2 alpha were labelled with the fluorescent Oregon Green Halotag ligand (HL-OregonGreen; Promega, WI, USA) to visualize the fusion protein. (c) Confocal images of C2C12 (mouse myoblast; top) & HEK293T (Human embryonic kidney cells; bottom) cells ectopically expressing EGFP-HIF-2 alpha . Scale bar, 5 μm. (d) HeLa cells transiently transfected with EGFP-HIF-2 alpha were imaged with a CCD camera. One thousand frames were acquired per cell in normoxia, hypoxia (1% v/v O2, 16 h) or following treatment with DMOG (0.5 mM, 6 h). The average (±s.d.) number of speckles per nucleus in each condition was 64 ± 49 (n = 25), 44 ± 24 (n = 24) & 96 ± 33 (n = 22), respectively. Mean of the sample data represented by the red dashed line. (e) Using the images from (d) the average speckle area per nucleus over the 1000 frames. The mean values (±s.d.) for each condition were 0.24 ± 0.09 µm (n = 25), 0.21 ± 0.07 µm (n = 24) & 0.27 ± 0.09 µm (n = 22), respectively. The mean values for hypoxia & DMOG were compared with the normoxic values (independent t-test, significance value set at 5%). Mean of the sample data represented by the red dashed line. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27655733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-124_mouse-monoclonal-arnt-hif-1-beta-antibody-h1beta234-310202415532083.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) - BSA Free [NB100-124] - PGE1 induces HIF-1 alpha protein accumulation in vascular-derived cells.Human aortic smooth muscle cells (HASMCs) (A & B), human umbilical vein endothelial cells (HUVECs) (C & D), & HEK293 cells (G) were exposed to 1 or 10 µM PGE1 under 20% O2 or 1% O2 conditions for 4 h. After treatment, cells were harvested & whole-cell lysates were subjected to immunoblot assay for HIF-1 alpha , HIF-1 beta , & beta -actin protein expression. Experiments were repeated thrice (A, C & G). Representative immunoblots are shown (A & C). Band intensities were analyzed densitometrically (B & D). Fold induction relative to lane 1 was plotted as mean ± S.D. ∗P < 0.05 compared with the control. HASMCs (E) & HUVECs (F) were exposed to 1 µM PGE1 for the indicated times under 20% O2 & were harvested for immunoblot assay for HIF-1 alpha protein. Experiments were repeated twice. Representative immunoblots are shown. (H) HASMCs & HUVECs were exposed to 10 µM PGE1 for 4 h under 20% O2 & were harvested for immunoblot assay for HIF-2 alpha protein. Experiments were repeated twice. Representative immunoblots are shown. I. HASMCs were exposed to 1 µM PGE1, lipo-PGE1 & PGE1-alfadex under 20% O2 conditions for 4 h. After treatment, cells were harvested & whole-cell lysates were subjected to immunoblot assay for HIF-1 alpha . Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24349900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-124_mouse-monoclonal-arnt-hif-1-beta-antibody-h1beta234-310202415541996.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) - BSA Free [NB100-124] - PGE1 induces HIF-1 alpha protein accumulation in vascular-derived cells.Human aortic smooth muscle cells (HASMCs) (A & B), human umbilical vein endothelial cells (HUVECs) (C & D), & HEK293 cells (G) were exposed to 1 or 10 µM PGE1 under 20% O2 or 1% O2 conditions for 4 h. After treatment, cells were harvested & whole-cell lysates were subjected to immunoblot assay for HIF-1 alpha , HIF-1 beta , & beta -actin protein expression. Experiments were repeated thrice (A, C & G). Representative immunoblots are shown (A & C). Band intensities were analyzed densitometrically (B & D). Fold induction relative to lane 1 was plotted as mean ± S.D. ∗P < 0.05 compared with the control. HASMCs (E) & HUVECs (F) were exposed to 1 µM PGE1 for the indicated times under 20% O2 & were harvested for immunoblot assay for HIF-1 alpha protein. Experiments were repeated twice. Representative immunoblots are shown. (H) HASMCs & HUVECs were exposed to 10 µM PGE1 for 4 h under 20% O2 & were harvested for immunoblot assay for HIF-2 alpha protein. Experiments were repeated twice. Representative immunoblots are shown. I. HASMCs were exposed to 1 µM PGE1, lipo-PGE1 & PGE1-alfadex under 20% O2 conditions for 4 h. After treatment, cells were harvested & whole-cell lysates were subjected to immunoblot assay for HIF-1 alpha . Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24349900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-124_mouse-monoclonal-arnt-hif-1-beta-antibody-h1beta234-31020241612659.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) - BSA Free [NB100-124] - PGE1 induces HIF-1 alpha protein accumulation in vascular-derived cells.Human aortic smooth muscle cells (HASMCs) (A & B), human umbilical vein endothelial cells (HUVECs) (C & D), & HEK293 cells (G) were exposed to 1 or 10 µM PGE1 under 20% O2 or 1% O2 conditions for 4 h. After treatment, cells were harvested & whole-cell lysates were subjected to immunoblot assay for HIF-1 alpha , HIF-1 beta , & beta -actin protein expression. Experiments were repeated thrice (A, C & G). Representative immunoblots are shown (A & C). Band intensities were analyzed densitometrically (B & D). Fold induction relative to lane 1 was plotted as mean ± S.D. ∗P < 0.05 compared with the control. HASMCs (E) & HUVECs (F) were exposed to 1 µM PGE1 for the indicated times under 20% O2 & were harvested for immunoblot assay for HIF-1 alpha protein. Experiments were repeated twice. Representative immunoblots are shown. (H) HASMCs & HUVECs were exposed to 10 µM PGE1 for 4 h under 20% O2 & were harvested for immunoblot assay for HIF-2 alpha protein. Experiments were repeated twice. Representative immunoblots are shown. I. HASMCs were exposed to 1 µM PGE1, lipo-PGE1 & PGE1-alfadex under 20% O2 conditions for 4 h. After treatment, cells were harvested & whole-cell lysates were subjected to immunoblot assay for HIF-1 alpha . Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24349900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-124_mouse-monoclonal-arnt-hif-1-beta-antibody-h1beta234-310202415535134.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody [NB100-110] - Analysis of HIF-1 beta using ARNT/HIF-1 beta antibody [NB100-110] in MCF7 whole cell lysate. Theoretical molecular weight is 86.6 kDa. Observed molecular weight ~95 kDa.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Western-Blot-NB100-110-img0001.jpg)
![Immunohistochemistry: ARNT/HIF-1 beta Antibody [NB100-110] - Immunohistochemical staining of human skin epidermis with ARNT/HIF-1 beta antibody [NB100-110].](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Immunohistochemistry-NB100-110-img0004.jpg)
![Chromatin Immunoprecipitation: ARNT/HIF-1 beta Antibody [NB100-110] - ChIP analysis with ARNT/HIF-1 beta antibody [NB100-110]. MDA-MB-231 cells were exposed to 20% or 1% O2 for 16 hours, and chromatin immunoprecipitation (ChIP) was performed with the indicated antibody (Ab). Primers flanking the HIF binding site were used for qPCR. ChIP image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Chromatin-Immunoprecipitation-NB100-110-img0009.jpg)
![Chromatin Immunoprecipitation (ChIP): ARNT/HIF-1 beta Antibody [NB100-110] - ChIP analysis using ARNT/HIF-1 beta antibody [NB100-110]. MCF7 were exposed to 20% or 1% O2 for 16h, and ChIP assays were performed using IgG or antibodies against HIF-1a, HIF-2a, HIF-1b. ChIP image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Chromatin-Immunoprecipitation-ChIP-NB100-110-img0006.jpg)
![Simple Western: ARNT/HIF-1 beta Antibody [NB100-110] - Simple Western analysis using ARNT/HIF-1 beta antibody [NB100-110]. Lane view shows a specific band for HIF-1 beta in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Theoretical molecular weight is 86.6 kDa. Observed molecular weight ~106 kDa.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Simple-Western-NB100-110-img0005.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Analysis of ARNT/HIF-1 beta using ARNT/HIF-1 beta antibody [NB100-110] from ARNT/HIF-1 beta antibody pack [NB100-982] in MCF7 whole cell lysate. Theoretical molecular weight of ARNT/HIF-1 beta is 86.6 kDa, observed molecular weight ~90 kDa.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Pack-Western-Blot-NB100-982-img0010.jpg "Western Blot: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Analysis of ARNT/HIF-1 beta using ARNT/HIF-1 beta antibody [NB100-110] from ARNT/HIF-1 beta antibody pack [NB100-982] in MCF7 whole cell lysate. Theoretical molecular weight of ARNT/HIF-1 beta is 86.6 kDa, observed molecular weight ~90 kDa.")
![Immunohistochemistry: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Staining of skin, epidermis using ARNT/HIF-1 beta antibody [NB100-110] from the ARNT/HIF-1 beta antibody pack [NB100-982].](http://images.novusbio.com/fullsize/HIF-1-beta-Antibody-Pack-Immunohistochemistry-NB100-982-img0005.jpg)
![Immunohistochemistry: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Staining of human glioblastoma multi-forme utilizing the ARNT/HIF-1 beta antibody[NB100-124] from the ARNT/HIF-1 beta antibody pack [NB100-982].](http://images.novusbio.com/fullsize/HIF-1-beta-Antibody-Pack-Immunohistochemistry-NB100-982-img0004.jpg)
NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for 
HAF017). A specific band was detected for ARNT/HIF-1 beta at approximately 100 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog # ![Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody [NBP2-54663] - Staining of human cell line A-431 shows localization to nucleus & nuclear bodies. Antibody staining using ARNT/HIF-1 beta antibody [NBP2-54663] is shown in green.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54663-img0002.jpg "Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody [NBP2-54663] - Staining of human cell line A-431 shows localization to nucleus & nuclear bodies. Antibody staining using ARNT/HIF-1 beta antibody [NBP2-54663] is shown in green.")
NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for ![SDS-Page: Recombinant Human ARNT/HIF-1 beta GST (N-Term) Protein [H00000405-P01] - SDS-PAGE analysis of Recombinant Human ARNT/HIF-1 beta GST (N-Term) protein on 12.5% gel and stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-ARNT-HIF-1-beta-Protein-SDS-Page-H00000405-P01-img0002.jpg "SDS-Page: Recombinant Human ARNT/HIF-1 beta GST (N-Term) Protein [H00000405-P01] - SDS-PAGE analysis of Recombinant Human ARNT/HIF-1 beta GST (N-Term) protein on 12.5% gel and stained with Coomassie Blue.")
![ELISA: Human ARNT/HIF-1 beta - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31945] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31945_human-arnt-hif-1-beta-ready-to-use-elisa-kit-color-2062024153396.png "ELISA: Human ARNT/HIF-1 beta - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31945] - Standard Curve Reference")
![Western Blot: ARNT/HIF-1 beta Antibody (2F11) [NBP3-15238] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate. All lanes: ARNT/HIF-1 beta antibody at 1.83ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 87, 85, 86 KDa. Observed band size: 87 KDa.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-2F11-Western-Blot-NBP3-15238-img0003.jpg "Western Blot: ARNT/HIF-1 beta Antibody (2F11) [NBP3-15238] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate. All lanes: ARNT/HIF-1 beta antibody at 1.83ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 87, 85, 86 KDa. Observed band size: 87 KDa.")
![Immunohistochemistry: ARNT/HIF-1 beta Antibody (2F11) [NBP3-15238] - IHC image of ARNT/HIF-1 beta antibody diluted at 1:183 and staining in paraffin-embedded human prostate cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-2F11-Immunohistochemistry-NBP3-15238-img0002.jpg)
![Immunoprecipitation: ARNT/HIF-1 beta Antibody (2F11) [NBP3-15238] - Immunoprecipitating HIF-1 beta in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of ARNT/HIF-1 beta antibody in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: ARNT/HIF-1 beta antibody (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-2F11-Immunoprecipitation-NBP3-15238-img0001.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody (S05-3B2) [NBP3-20084] - Western blot detection of ARNT/HIF-1 beta in Hela (Lane 1), CHO-K1 (Lane 2), C6 (Lane 3), Ramos (Lane 4) using NBP3-20084 (1:1000 diluted)](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-S05-3B2-Western-Blot-NBP3-20084-img0002.jpg "Western Blot: ARNT/HIF-1 beta Antibody (S05-3B2) [NBP3-20084] - Western blot detection of ARNT/HIF-1 beta in Hela (Lane 1), CHO-K1 (Lane 2), C6 (Lane 3), Ramos (Lane 4) using NBP3-20084 (1:1000 diluted)")
![Immunohistochemistry-Paraffin: ARNT/HIF-1 beta Antibody (S05-3B2) [NBP3-20084] - Immunohistochemistry of ARNT/HIF-1 beta in paraffin-embedded Human lung cancer tissue using NBP3-20084 at dilution 1/50](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-S05-3B2-Immunohistochemistry-Paraffin-NBP3-20084-img0001.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody (3R0Y4) [NBP3-15757] - Western blot analysis of extracts of HeLa cells, using ARNT/HIF-1 beta antibody (NBP3-15757) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-3R0Y4-Western-Blot-NBP3-15757-img0002.jpg "Western Blot: ARNT/HIF-1 beta Antibody (3R0Y4) [NBP3-15757] - Western blot analysis of extracts of HeLa cells, using ARNT/HIF-1 beta antibody (NBP3-15757) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: ARNT/HIF-1 beta Antibody (3R0Y4) [NBP3-15757] - Immunohistochemistry of paraffin-embedded human colon using ARNT/HIF-1 beta antibody (NBP3-15757) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-3R0Y4-Immunohistochemistry-Paraffin-NBP3-15757-img0001.jpg)
