Products Related to Gene-ID: 3592

309-IL) is present at 0.25 µg/mL." class="big_lightbox"> 309-IL) is present at 0.25 µg/mL." alt="Measured by its ability to induce IFN-gamma secretion in NK-92 human natural killer lymphoma cells, the concentration of Recombinant Human IL‑12 p35 (C96S) Monomer (Catalog # 11209-IL) that produces 50% of the response is 0.300-3.00 ng/mL when Recombinant Human IL‑12 p40 Monomer (309-IL) is present at 0.25 µg/mL." class="big_thumb" />

839-B1) is immobilized at 1.00 µg/mL (100µL/well), Biotinylated Recombinant Human IL‑12 Protein (Catalog # BT10018) binds with an ED₅₀ of 40.0-400 ng/mL" class="big_lightbox"> 839-B1) is immobilized at 1.00 µg/mL (100µL/well), Biotinylated Recombinant Human IL‑12 Protein (Catalog # BT10018) binds with an ED₅₀ of 40.0-400 ng/mL" alt="Measured by its binding ability in a functional ELISA. When Recombinant Human IL-12 R beta 1 Fc Chimera Protein (839-B1) is immobilized at 1.00 µg/mL (100µL/well), Biotinylated Recombinant Human IL‑12 Protein (Catalog # BT10018) binds with an ED₅₀ of 40.0-400 ng/mL" class="big_thumb" />

F0113) and subsequently Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> F0113) and subsequently Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." alt="PBMC monocytes treated with IFN-gamma (Catalog # 285-IF, 10 ng/mL for 2 hours), then LPS (100 ng/mL for 12 hours), and lastly monensin (3 µM for 3 hours) (A) vs naïve PBMC monocytes (B) were stained with Rat Anti-Human IL‑12 Monoclonal Antibody (Catalog # MAB11485) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113) and subsequently Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />