| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90685-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-22408-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49406-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP2-79602-5Plates | |||
![Western Blot: IFN-gamma R1/CD119 Antibody (JE40-50) [NBP3-32458] - Western blot analysis of IFN-gamma R1/CD119 on different lysates with Rabbit anti-IFN-gamma R1/CD119 antibody (NBP3-32458) at 1/1,000 dilution. Lane 1: NCI-H226 cell lysate (20 ug/Lane) Lane 2: LoVo cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: THP-1 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: MCF7 cell lysate (negative) (20 ug/Lane) Lane 8: HL-60 cell lysate (20 ug/Lane) Lane 9: SW620 cell lysate (20 ug/Lane) Lane 10: Mouse liver tissue lysate (40 ug/Lane) Lane 11: Mouse thymus tissue lysate (40 ug/Lane) Predicted band size: 54 kDa Observed band size: 40-100 kDa Exposure time: 1 minute 2 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32458) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32458_rabbit-ifn-gamma-r1-cd119-mab-je40-50-278202415242680.jpg "Western Blot: IFN-gamma R1/CD119 Antibody (JE40-50) [NBP3-32458] - Western blot analysis of IFN-gamma R1/CD119 on different lysates with Rabbit anti-IFN-gamma R1/CD119 antibody (NBP3-32458) at 1/1,000 dilution. Lane 1: NCI-H226 cell lysate (20 ug/Lane) Lane 2: LoVo cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: THP-1 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: MCF7 cell lysate (negative) (20 ug/Lane) Lane 8: HL-60 cell lysate (20 ug/Lane) Lane 9: SW620 cell lysate (20 ug/Lane) Lane 10: Mouse liver tissue lysate (40 ug/Lane) Lane 11: Mouse thymus tissue lysate (40 ug/Lane) Predicted band size: 54 kDa Observed band size: 40-100 kDa Exposure time: 1 minute 2 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32458) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: IFN-gamma R1/CD119 Antibody (JE40-50) [NBP3-32458] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-IFN-gamma R1/CD119 antibody (NBP3-32458) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32458) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32458_rabbit-ifn-gamma-r1-cd119-mab-je40-50-298202414333652.jpg)
![Flow Cytometry: IFN-gamma R1/CD119 Antibody (062) [NBP2-90685] - Flow cytometric analysis of Mouse IFNGR1(CD119) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse IFNGR1(CD119), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/IFN-gamma-R1-CD119-Antibody-062-Flow-Cytometry-NBP2-90685-img0002.jpg "Flow Cytometry: IFN-gamma R1/CD119 Antibody (062) [NBP2-90685] - Flow cytometric analysis of Mouse IFNGR1(CD119) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse IFNGR1(CD119), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/ Immunofluorescence: IFN-gamma R1/CD119 Antibody (062) [NBP2-90685] -staining of mouse IFN-gamma R1/CD119 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90685 (1:60) at 4C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-90685_rabbit-monoclonal-ifn-gamma-r1-cd119-antibody-062-235202521451779.png)
![Western Blot: IFN-gamma R1/CD119 Antibody (2B9Q1) [IFN-gamma R1/CD119] - Western blot analysis of lysates from wild type(WT) and IFN-gamma R1/CD119 knockout (KO) 293T cells, using [KO Validated] IFN-gamma R1/CD119 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Enhanced Kit .Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-15421_rabbit-ifn-gamma-r1-cd119-mab-2b9q1-52202517223929.jpg "<b>Genetic Strategies Validation. </b>Western Blot: IFN-gamma R1/CD119 Antibody (2B9Q1) [IFN-gamma R1/CD119] - Western blot analysis of lysates from wild type(WT) and IFN-gamma R1/CD119 knockout (KO) 293T cells, using [KO Validated] IFN-gamma R1/CD119 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Enhanced Kit .Exposure time: 180s.")
![<b>Genetic Strategies Validation. </b>Analysis of various lysates, using [KO Validated] IFNGR1 Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-15421_rabbit-ifn-gamma-r1-cd119-mab-2b9q1-western-blot-25112025135530.png)
![Immunohistochemistry-Paraffin: IFN-gamma R1/CD119 Antibody (RM0093-7K23) [NBP1-22408] - The Kidney tissue samples from the Folic Acid induced kidney injury model were fixed using 4% PFA at 4C for overnight and embeded in paraffin. 4um section was sublected to IHC with NBP1-22408](http://images.novusbio.com/fullsize/IFN-gamma-R1-CD119-Antibody-RM0093-7K23-Immunohistochemistry-Paraffin-NBP1-22408-img0001.jpg "Immunohistochemistry-Paraffin: IFN-gamma R1/CD119 Antibody (RM0093-7K23) [NBP1-22408] - The Kidney tissue samples from the Folic Acid induced kidney injury model were fixed using 4% PFA at 4C for overnight and embeded in paraffin. 4um section was sublected to IHC with NBP1-22408")
![Immunohistochemistry-Paraffin: IFN-gamma R1/CD119 Antibody [NBP2-49406] - Staining of human spleen shows strong membranous positivity in cells in red pulp.](http://images.novusbio.com/fullsize/IFN-gamma-R1-CD119-Antibody-Immunohistochemistry-Paraffin-NBP2-49406-img0005.jpg "Immunohistochemistry-Paraffin: IFN-gamma R1/CD119 Antibody [NBP2-49406] - Staining of human spleen shows strong membranous positivity in cells in red pulp.")
![ELISA: Mouse IFN-gamma R1/CD119 ELISA Kit (Colorimetric) [NBP3-06817] - Samples were spiked with high concentrations of Mouse IFN-gamma R1/CD119 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06817_mouse-ifn-gamma-r1-cd119-elisa-kit-colorimetric-132202416133663.jpg "ELISA: Mouse IFN-gamma R1/CD119 ELISA Kit (Colorimetric) [NBP3-06817] - Samples were spiked with high concentrations of Mouse IFN-gamma R1/CD119 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse IFN-gamma R1/CD119 ELISA Kit (Colorimetric) [NBP3-06817] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06817_mouse-ifn-gamma-r1-cd119-elisa-kit-colorimetric-4320249133259.jpg)
![Western Blot: IFNGR1 Overexpression Lysate (Adult Normal) [NBL1-11852] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IFNGR1.](http://images.novusbio.com/fullsize/IFNGR1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11852-img0002.jpg "Western Blot: IFNGR1 Overexpression Lysate (Adult Normal) [NBL1-11852] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IFNGR1.")
![Western Blot: IFN-gamma R1/CD119 Antibody (S08-9H9) [NBP3-14884] - Western blot detection of IFN-gamma R1/CD119 in K562, C6, 3T3, Hela cell lysates using IFN-gamma R1/CD119 Rabbit mAb (1:1000 diluted). Predicted band size: 54kDa. Observed band size: 100kDa.](http://images.novusbio.com/fullsize/IFN-gamma-R1-CD119-Antibody-S08-9H9-Western-Blot-NBP3-14884-img0001.jpg "Western Blot: IFN-gamma R1/CD119 Antibody (S08-9H9) [NBP3-14884] - Western blot detection of IFN-gamma R1/CD119 in K562, C6, 3T3, Hela cell lysates using IFN-gamma R1/CD119 Rabbit mAb (1:1000 diluted). Predicted band size: 54kDa. Observed band size: 100kDa.")
![Western Blot: IFN-gamma R1/CD119 Antibody (C3) [NBP3-41089] - Sample: 293T cell lysate Primary Ab: 0.2ug/ml NBP3-41089; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41089_mouse-ifn-gamma-r1-cd119-mab-c3-612202421351219.jpg "Western Blot: IFN-gamma R1/CD119 Antibody (C3) [NBP3-41089] - Sample: 293T cell lysate Primary Ab: 0.2ug/ml NBP3-41089; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Sandwich ELISA: IFN-gamma R1/CD119 Antibody Pair [HRP] [NBP2-79602] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/IFN-gamma-R1-CD119-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79602-img0001.jpg "Sandwich ELISA: IFN-gamma R1/CD119 Antibody Pair [HRP] [NBP2-79602] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")