Immunoblot Buffer Group 8." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and mouse lactating tissues. PVDF membrane was probed with 1 µg/mL of Chicken Anti-Human/Mouse HMGB1/HMG‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1690) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. A specific band was detected for HMGB1/HMG‑1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1690-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-34960-1mg | |||
| Carrier-Free | NBP2-34960-100ug | |||
| Carrier-Free | NBP2-34960-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-15486-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27169SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27400SS | |||
| Azide and BSA Free | NBP2-80774 | |||
NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="HMGB1/HMG-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse HMGB1/HMG-1 Monoclonal Antibody (Catalog # MAB16902) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB16902-SP | |||
1145-RG) with an ED50 of 0.040-0.400 μg/mL." alt="Measured by its binding ability in a functional ELISA. Recombinant Human HMGB1/HMG‑1 His-tag Protein (Catalog # 11803-HM) binds to Recombinant Human RAGE Fc Chimera Protein (1145-RG) with an ED50 of 0.040-0.400 μg/mL." class="big_thumb" />
AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using ![Western Blot: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Western blot analysis of lysates from wild type (WT) and HMGB1/HMG-1 knockout (KO) HeLa cells using [KO Validated] HMGB1/HMG-1 Rabbit mAb at 1:6000 dilution incubated overnight at 4C.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 0.5s.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-52202517352784.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Western blot analysis of lysates from wild type (WT) and HMGB1/HMG-1 knockout (KO) HeLa cells using [KO Validated] HMGB1/HMG-1 Rabbit mAb at 1:6000 dilution incubated overnight at 4C.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 0.5s.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (1C4T5) [NBP3-15755] - Confocal imaging of NIH-3T3 cells using [KO Validated] HMGB1 Rabbit mAb (dilution 1:100) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-immunocytochemistry-immunofluorescence-1962023104824.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Immunohistochemistry analysis of paraffin-embedded Rat liver using [KO Validated] HMGB1/HMG-1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-52202517344263.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using [KO Validated] HMGB1/HMG-1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-52202517311012.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] HMGB1/HMG-1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-52202517304984.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using [KO Validated] HMGB1/HMG-1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-52202517311085.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Immunohistochemistry analysis of paraffin-embedded Mouse colon using [KO Validated] HMGB1/HMG-1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-5220251734575.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using [KO Validated] HMGB1/HMG-1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-52202517352740.jpg)
![Western Blot: HMGB1/HMG-1 Antibody (1C4T5) [NBP3-15755] - Western blot analysis of various lysates using [KO Validated] HMGB1/HMG-1 Rabbit mAb at 1:6000 dilution incubated overnight at 4C.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 0.5s.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-8220252010416.jpg)
![ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Chemiluminescence) [NBP2-62782] - Samples were spiked with high concentrations of Mouse HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-62782_mouse-hmgb1-hmg-1-elisa-kit-chemiluminescence-13220241624343.jpg "ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Chemiluminescence) [NBP2-62782] - Samples were spiked with high concentrations of Mouse HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Chemiluminescence) [NBP2-62782] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-62782_mouse-hmgb1-hmg-1-elisa-kit-chemiluminescence-4320249151663.jpg)
![Western Blot: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis using HMGB1 antibody. Human ThP1 cell lysate in the (1) absence and (2) presence of immunizing peptide, as well as (3) mouse NIH 3T3 cell lysate were probed using HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Western-Blot-NBP2-27117-img0008.jpg "Western Blot: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis using HMGB1 antibody. Human ThP1 cell lysate in the (1) absence and (2) presence of immunizing peptide, as well as (3) mouse NIH 3T3 cell lysate were probed using HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis of decalcified, FFPE tissue sections from the paws of mice with collagen-induced arthritis using the HMGB1 antibody. The antibody was used at 5 ug/ml. Data courtesy of Dr Ulf Andersson, Karolinska Institute.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Immunohistochemistry-Paraffin-NBP2-27117-img0007.jpg)
![Flow Cytometry: HMGB1/HMG-1 Antibody [NBP2-27117] - Intracellular analysis using HMGB1 antibody. Human monocytes were probed using 0.5 ug of HMGB1 antibody (red) and 0.5 ug of isotype control antibody (green). NBP2-27117 and APC secondary this antibody were used for this test.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Flow-Cytometry-NBP2-27117-img0006.jpg)
![Simple Western: HMGB1/HMG-1 Antibody [NBP2-27117] - Lane view shows a specific band for HMGB1 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Simple-Western-NBP2-27117-img0005.jpg)
![ELISA: Human HMGB1/HMG-1 ELISA Kit (Chemiluminescence) [NBP2-62781] - Samples were spiked with high concentrations of Human HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-62781_human-hmgb1-hmg-1-elisa-kit-chemiluminescence-132202416225284.jpg "ELISA: Human HMGB1/HMG-1 ELISA Kit (Chemiluminescence) [NBP2-62781] - Samples were spiked with high concentrations of Human HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human HMGB1/HMG-1 ELISA Kit (Chemiluminescence) [NBP2-62781] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-62781_human-hmgb1-hmg-1-elisa-kit-chemiluminescence-43202495079.jpg)
![ELISA: Human HMGB1/HMG-1 ELISA Kit (Chemiluminescence) [NBP2-62781] - Standard Curve from ELISA tested on Human cell culture supernatants. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-62781_human-hmgb1-hmg-1-elisa-kit-chemiluminescence-elisa-263202414748..jpg)
![SDS-Page: Human HMGB1/HMG-1 His (C-Term) Protein [NBP2-34960]](http://images.novusbio.com/fullsize/Human-HMGB1-HMG-1-His-C-Term-Protein-SDS-Page-NBP2-34960-img0001.jpg "SDS-Page: Human HMGB1/HMG-1 His (C-Term) Protein [NBP2-34960]")
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 [ac Lys29] Antibody [NBP3-15486] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] HMGB1/HMG-1 Rabbit pAb (NBP3-15486) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-[ac-Lys29]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15486-img0003.jpg "Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 [ac Lys29] Antibody [NBP3-15486] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] HMGB1/HMG-1 Rabbit pAb (NBP3-15486) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 [ac Lys29] Antibody [NBP3-15486] - Immunofluorescence analysis of C6 cells using [KO Validated] HMGB1/HMG-1 Rabbit pAb (NBP3-15486) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-[ac-Lys29]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15486-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 [ac Lys29] Antibody [NBP3-15486] - Immunofluorescence analysis of U-2 OS cells using [KO Validated] HMGB1/HMG-1 Rabbit pAb (NBP3-15486) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-[ac-Lys29]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15486-img0004.jpg)
![Western Blot: HMGB1/HMG-1 Antibody [NBP2-27169] - Analysis using HMGB1 antibody. Mouse NIH 3T3 cell lysate was probed with HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Western-Blot-NBP2-27169-img0003.jpg "Western Blot: HMGB1/HMG-1 Antibody [NBP2-27169] - Analysis using HMGB1 antibody. Mouse NIH 3T3 cell lysate was probed with HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Simple Western: HMGB1/HMG-1 Antibody [NBP2-27169] - Simple Western lane view shows a specific band for HMGB1 in 0.2 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Simple-Western-NBP2-27169-img0002.jpg)
![Western Blot: HMGB1/HMG-1 Antibody (19N12A1) [NBP2-27400] - Analysis using HMGB1 antibody. A) Full-length human HMGB1 protein, B) human HeLa cell lysate and C) mouse NIH 3T3 cell lysate were probed with HMGB1 antibody at 2 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N12A1-Western-Blot-NBP2-27400-img0004.jpg "Western Blot: HMGB1/HMG-1 Antibody (19N12A1) [NBP2-27400] - Analysis using HMGB1 antibody. A) Full-length human HMGB1 protein, B) human HeLa cell lysate and C) mouse NIH 3T3 cell lysate were probed with HMGB1 antibody at 2 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Simple Western: HMGB1/HMG-1 Antibody (19N12A1) [NBP2-27400] - Image shows a specific band for HMGB1 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N12A1-Simple-Western-NBP2-27400-img0003.jpg)
![SDS-Page: Recombinant Human HMGB1/HMG-1 Protein [NBC1-18488] - HMGB1, 25kDa (223aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE.](http://images.novusbio.com/fullsize/Recombinant-Human-HMGB1-HMG-1-Protein-SDS-Page-NBC1-18488-img0002.jpg "SDS-Page: Recombinant Human HMGB1/HMG-1 Protein [NBC1-18488] - HMGB1, 25kDa (223aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE.")
