HAF109). A specific band was detected for HCLS1 at approximately 75 to 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of L1.2 mouse pro-B cell line, Nb2-11 rat lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat HCLS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5789) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HCLS1 at approximately 75 to 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5789-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85380-25ul | |||
![Western Blot: HCLS1 Antibody (1A8) [H00003059-M02] - Analysis of HCLS1 expression in transfected 293T cell line by HCLS1 monoclonal antibody (M02), clone 1A8.Lane 1: HCLS1 transfected lysate(54 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HCLS1-Antibody-1A8-Western-Blot-H00003059-M02-img0014.jpg "Western Blot: HCLS1 Antibody (1A8) [H00003059-M02] - Analysis of HCLS1 expression in transfected 293T cell line by HCLS1 monoclonal antibody (M02), clone 1A8.Lane 1: HCLS1 transfected lysate(54 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: HCLS1 Antibody (1A8) [H00003059-M02] - Analysis of monoclonal antibody to HCLS1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/HCLS1-Antibody-1A8-Immunohistochemistry-Paraffin-H00003059-M02-img0010.jpg)
![Western Blot: HCLS1 Antibody (1A8) [H00003059-M02] - Analysis of HCLS1 over-expressed 293 cell line, cotransfected with HCLS1 Validated Chimera RNAi ( Cat # H00003059-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HCLS1 monoclonal antibody (M02), clone 1A8 (Cat # H00003059-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/HCLS1-Antibody-1A8-Western-Blot-H00003059-M02-img0011.jpg)
![Western Blot: HCLS1 Antibody (1A8) [H00003059-M02] - HCLS1 monoclonal antibody (M02), clone 1A8 Analysis of HCLS1 expression in K-562.](http://images.novusbio.com/fullsize/HCLS1-Antibody-1A8-Western-Blot-H00003059-M02-img0013.jpg)
![Proximity Ligation Assay: HCLS1 Antibody (1A8) [H00003059-M02] - Analysis of protein-protein interactions between CASP3 and HCLS1. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-HCLS1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/HCLS1-Antibody-1A8-Proximity-Ligation-Assay-H00003059-M02-img0008.jpg)
![Sandwich ELISA: HCLS1 Antibody (1A8) [H00003059-M02] - Detection limit for recombinant GST tagged HCLS1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HCLS1-Antibody-1A8-Sandwich-ELISA-H00003059-M02-img0012.jpg)
![Western Blot: HCLS1 Antibody (2A9) [H00003059-M06] - Analysis of HCLS1 expression in K-562 (Cat # L009V1).](http://images.novusbio.com/fullsize/HCLS1-Antibody-2A9-Western-Blot-H00003059-M06-img0007.jpg "Western Blot: HCLS1 Antibody (2A9) [H00003059-M06] - Analysis of HCLS1 expression in K-562 (Cat # L009V1).")
![Immunocytochemistry/Immunofluorescence: HCLS1 Antibody (2A9) [H00003059-M06] - Analysis of monoclonal antibody to HCLS1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/HCLS1-Antibody-2A9-Immunocytochemistry-Immunofluorescence-H00003059-M06-img0005.jpg)
![Immunohistochemistry-Paraffin: HCLS1 Antibody (2A9) [H00003059-M06] - Analysis of monoclonal antibody to HCLS1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/HCLS1-Antibody-2A9-Immunohistochemistry-Paraffin-H00003059-M06-img0006.jpg)
![ELISA: HCLS1 Antibody (2A9) [H00003059-M06] - Detection limit for recombinant GST tagged HCLS1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HCLS1-Antibody-2A9-ELISA-H00003059-M06-img0004.jpg)
![Western Blot: HCLS1 Antibody [NBP1-30953] - Sample (30 ug of whole cell lysate) A: Jurkat B: HL-60 7. 5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/HCLS1-Antibody-Western-Blot-NBP1-30953-img0004.jpg "Western Blot: HCLS1 Antibody [NBP1-30953] - Sample (30 ug of whole cell lysate) A: Jurkat B: HL-60 7. 5% SDS PAGE; antibody diluted at 1:10000.")
![Immunohistochemistry-Paraffin: HCLS1 Antibody [NBP1-30953] - Paraffin-embedded Lymphocytes in gastric epithelium, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/HCLS1-Antibody-Immunohistochemistry-Paraffin-NBP1-30953-img0002.jpg)
![Western Blot: HCLS1 Antibody [NBP1-30953] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/HCLS1-Antibody-Western-Blot-NBP1-30953-img0003.jpg)
![Western Blot: HCLS1 Antibody (2A6) [H00003059-M03] - Analysis of HCLS1 expression in K-562 (Cat # L009V1).](http://images.novusbio.com/fullsize/HCLS1-Antibody-2A6-Western-Blot-H00003059-M03-img0007.jpg "Western Blot: HCLS1 Antibody (2A6) [H00003059-M03] - Analysis of HCLS1 expression in K-562 (Cat # L009V1).")
![Immunohistochemistry-Paraffin: HCLS1 Antibody (2A6) [H00003059-M03] - Analysis of monoclonal antibody to HCLS1 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/HCLS1-Antibody-2A6-Immunohistochemistry-Paraffin-H00003059-M03-img0005.jpg)
![Sandwich ELISA: HCLS1 Antibody (2A6) [H00003059-M03] - Detection limit for recombinant GST tagged HCLS1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HCLS1-Antibody-2A6-Sandwich-ELISA-H00003059-M03-img0006.jpg)
![Western Blot: HCLS1 Antibody (3D5) [H00003059-M01] - HCLS1 monoclonal antibody (M01), clone 3D5 Analysis of HCLS1 expression in K-562.](http://images.novusbio.com/fullsize/HCLS1-Antibody-3D5-Western-Blot-H00003059-M01-img0006.jpg "Western Blot: HCLS1 Antibody (3D5) [H00003059-M01] - HCLS1 monoclonal antibody (M01), clone 3D5 Analysis of HCLS1 expression in K-562.")
![Immunocytochemistry/Immunofluorescence: HCLS1 Antibody (3D5) [H00003059-M01] - Analysis of monoclonal antibody to HCLS1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/HCLS1-Antibody-3D5-Immunocytochemistry-Immunofluorescence-H00003059-M01-img0004.jpg)
![Sandwich ELISA: HCLS1 Antibody (3D5) [H00003059-M01] - Detection limit for recombinant GST tagged HCLS1 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HCLS1-Antibody-3D5-Sandwich-ELISA-H00003059-M01-img0005.jpg)
![Western Blot: HCLS1 Antibody (3F4) [H00003059-M04] - Analysis of HCLS1 expression in K-562 (Cat # L009V1).](http://images.novusbio.com/fullsize/HCLS1-Antibody-3F4-Western-Blot-H00003059-M04-img0005.jpg "Western Blot: HCLS1 Antibody (3F4) [H00003059-M04] - Analysis of HCLS1 expression in K-562 (Cat # L009V1).")
![Immunohistochemistry-Paraffin: HCLS1 Antibody (3F4) [H00003059-M04] - Analysis of monoclonal antibody to HCLS1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/HCLS1-Antibody-3F4-Immunohistochemistry-Paraffin-H00003059-M04-img0004.jpg)
![ELISA: HCLS1 Antibody (3F4) [H00003059-M04] - Detection limit for recombinant GST tagged HCLS1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HCLS1-Antibody-3F4-ELISA-H00003059-M04-img0003.jpg)
![Western Blot: HCLS1 Antibody [NBP2-16787] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/HCLS1-Antibody-Western-Blot-NBP2-16787-img0001.jpg "Western Blot: HCLS1 Antibody [NBP2-16787] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: HCLS1 Antibody [NBP2-04028] - Detection of human HS1 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-HS1 antibody NBP2-04028 used for WB at 1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. HS1 was also immunoprecipitated by another rabbit anti-HS1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/HCLS1-Antibody-Western-Blot-NBP2-04028-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: HCLS1 Antibody [NBP2-04028] - Detection of human HS1 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-HS1 antibody NBP2-04028 used for WB at 1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. HS1 was also immunoprecipitated by another rabbit anti-HS1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).")
![Immunohistochemistry-Paraffin: HCLS1 Antibody [NBP1-85380] - Staining of human spleen shows strong cytoplasmic positivity in cells of white pulp.](http://images.novusbio.com/fullsize/HCLS1-Antibody-Immunohistochemistry-Paraffin-NBP1-85380-img0006.jpg "Immunohistochemistry-Paraffin: HCLS1 Antibody [NBP1-85380] - Staining of human spleen shows strong cytoplasmic positivity in cells of white pulp.")
