| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74750 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38343-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22630-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74750 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74750 | |||
![Western Blot: UBE2T Antibody (JE65-79) [NBP3-33076] - Western blot analysis of UBE2T on different lysates with Rabbit anti-UBE2T antibody (NBP3-33076) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 ug/Lane) Lane 2: K-562 cell lysate (15 ug/Lane) Lane 3: MCF7 cell lysate (15 ug/Lane) Lane 4: A549 cell lysate (15 ug/Lane) Lane 5: HEK-293 cell lysate (15 ug/Lane) Lane 6: Jurkat cell lysate (15 ug/Lane) Lane 7: SKOV-3 cell lysate (15 ug/Lane) Lane 8: SH-SY5Y cell lysate (15 ug/Lane) Lane 9: HepG2 cell lysate (15 ug/Lane) Lane 10: Mouse testis tissue lysate (30 ug/Lane) Lane 11: Rat testis tissue lysate (30 ug/Lane) Predicted band size: 22.5 kDa Observed band size: 22.5 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33076) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33076_rabbit-ube2t-mab-je65-79-288202414191523.jpg "Western Blot: UBE2T Antibody (JE65-79) [NBP3-33076] - Western blot analysis of UBE2T on different lysates with Rabbit anti-UBE2T antibody (NBP3-33076) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 ug/Lane) Lane 2: K-562 cell lysate (15 ug/Lane) Lane 3: MCF7 cell lysate (15 ug/Lane) Lane 4: A549 cell lysate (15 ug/Lane) Lane 5: HEK-293 cell lysate (15 ug/Lane) Lane 6: Jurkat cell lysate (15 ug/Lane) Lane 7: SKOV-3 cell lysate (15 ug/Lane) Lane 8: SH-SY5Y cell lysate (15 ug/Lane) Lane 9: HepG2 cell lysate (15 ug/Lane) Lane 10: Mouse testis tissue lysate (30 ug/Lane) Lane 11: Rat testis tissue lysate (30 ug/Lane) Predicted band size: 22.5 kDa Observed band size: 22.5 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33076) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: UBE2T Antibody (JE65-79) [NBP3-33076] - Immunocytochemistry analysis of HeLa cells labeling UBE2T with Rabbit anti-UBE2T antibody (NBP3-33076) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-UBE2T antibody (NBP3-33076) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33076_rabbit-ube2t-mab-je65-79-2920241048488.jpg)
![Western Blot: UBE2T Antibody (2F5) [NBP2-02965] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2T (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2T.](http://images.novusbio.com/fullsize/UBE2T-Antibody-2F5-Western-Blot-NBP2-02965-img0001.jpg "Western Blot: UBE2T Antibody (2F5) [NBP2-02965] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2T (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2T.")
![Immunocytochemistry/Immunofluorescence: UBE2T Antibody (2F5) [NBP2-02965] Staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2T.](http://images.novusbio.com/fullsize/UBE2T-Antibody-2F5-Immunocytochemistry-Immunofluorescence-NBP2-02965-img0002.jpg)
![Western Blot: UBE2T Antibody (1E12-4A3) [H00029089-M01] - UBE2T monoclonal antibody (M01), clone 1E12-4A3 Analysis of UBE2T expression in Hela.](http://images.novusbio.com/fullsize/UBE2T-Antibody-1E12-4A3-Western-Blot-H00029089-M01-img0003.jpg "Western Blot: UBE2T Antibody (1E12-4A3) [H00029089-M01] - UBE2T monoclonal antibody (M01), clone 1E12-4A3 Analysis of UBE2T expression in Hela.")
![Immunoprecipitation: UBE2T Antibody (1E12-4A3) [H00029089-M01] - Analysis of UBE2T transfected lysate using anti-UBE2T monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with UBE2T MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/UBE2T-Antibody-1E12-4A3-Immunoprecipitation-H00029089-M01-img0002.jpg)
![Western Blot: UBE2T Antibody [NB100-1150] - Analysis of UBE2T in K562 lysate (35ug protein in RIPA buffer) using NB100-1150 at 0.1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/UBE2T-Antibody-Western-Blot-NB100-1150-img0001.jpg "Western Blot: UBE2T Antibody [NB100-1150] - Analysis of UBE2T in K562 lysate (35ug protein in RIPA buffer) using NB100-1150 at 0.1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: UBE2T Antibody [NBP3-38343] - Western blot analysis of various lysates using UBE2T Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38343_rabbit-ube2t-pab-101220241136468.jpg "Western Blot: UBE2T Antibody [NBP3-38343] - Western blot analysis of various lysates using UBE2T Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Western Blot: UBE2T Antibody (4G1-4C2) [H00029089-M02] - Analysis of UBE2T expression in transfected 293T cell line by UBE2T monoclonal antibody (M02), clone 4G1-4C2.Lane 1: UBE2T transfected lysate(22.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/UBE2T-Antibody-4G1-4C2-Western-Blot-H00029089-M02-img0002.jpg "Western Blot: UBE2T Antibody (4G1-4C2) [H00029089-M02] - Analysis of UBE2T expression in transfected 293T cell line by UBE2T monoclonal antibody (M02), clone 4G1-4C2.Lane 1: UBE2T transfected lysate(22.5 KDa).Lane 2: Non-transfected lysate.")
![Immunoprecipitation: UBE2T Antibody (4G1-4C2) [H00029089-M02] - Analysis of UBE2T transfected lysate using anti-UBE2T monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with UBE2T MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/UBE2T-Antibody-4G1-4C2-Immunoprecipitation-H00029089-M02-img0001.jpg)
![Western Blot: UBE2T Antibody (2A12-4F11) [H00029089-M03] - Analysis of UBE2T expression in transfected 293T cell line by UBE2T monoclonal antibody (M03), clone 2A12-4F11. Lane 1: UBE2T transfected lysatE (22.5 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/UBE2T-Antibody-2A12-4F11-Western-Blot-H00029089-M03-img0002.jpg "Western Blot: UBE2T Antibody (2A12-4F11) [H00029089-M03] - Analysis of UBE2T expression in transfected 293T cell line by UBE2T monoclonal antibody (M03), clone 2A12-4F11. Lane 1: UBE2T transfected lysatE (22.5 KDa). Lane 2: Non-transfected lysate.")
![Immunoprecipitation: UBE2T Antibody (2A12-4F11) [H00029089-M03] - Analysis of UBE2T transfected lysate using anti-UBE2T monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with UBE2T rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/UBE2T-Antibody-2A12-4F11-Immunoprecipitation-H00029089-M03-img0001.jpg)
![Western Blot: UBE2T Overexpression Lysate (Adult Normal) [NBL1-17545] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBE2T.](http://images.novusbio.com/fullsize/UBE2T-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17545-img0002.jpg "Western Blot: UBE2T Overexpression Lysate (Adult Normal) [NBL1-17545] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBE2T.")
![Western Blot: UBE2T Antibody (SR1361) [NBP3-22630] - Western blot analysis using NBP3-22630 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22630_rabbit-ube2t-mab-sr1361-57202315234918.jpg "Western Blot: UBE2T Antibody (SR1361) [NBP3-22630] - Western blot analysis using NBP3-22630 in HeLa cell lysate.")