| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85574-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92908-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49321-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22596-50ul | |||
![Western Blot: CLIC4 Antibody [NBP1-00172] - (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Western-Blot-NBP1-00172-img0004.jpg "Western Blot: CLIC4 Antibody [NBP1-00172] - (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CLIC4 Antibody [NBP1-00172] - (5ug/ml) staining of min6 cells transiently expressing mouse Clic4. Nuclear counter staining by Hoechst. Data kindly provided by Dr. Virginie Nepote, Lausanne University, Switzerland.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00172-img0001.jpg)
![Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-00172] - (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunohistochemistry-Paraffin-NBP1-00172-img0003.jpg)
![Western Blot: CLIC4 Antibody [NBP2-92908] - Analysis of extracts of various cell lines, using CLIC4 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Western-Blot-NBP2-92908-img0003.jpg "Western Blot: CLIC4 Antibody [NBP2-92908] - Analysis of extracts of various cell lines, using CLIC4 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: CLIC4 Antibody [NBP2-92908] - Analysis of A-549 cells using CLIC4 . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92908-img0001.jpg)
![Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP2-92908] - Rat kidney using CLIC4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunohistochemistry-Paraffin-NBP2-92908-img0006.jpg)
![Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP2-92908] - Human esophageal cancer using CLIC4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunohistochemistry-Paraffin-NBP2-92908-img0004.jpg)
![Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP2-92908] - Mouse kidney using CLIC4 Rabbit pAb dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunohistochemistry-Paraffin-NBP2-92908-img0005.jpg)
![Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP2-49321] - Staining of human skeletal muscle using Anti-CLIC4 antibody NBP2-49321.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunohistochemistry-Paraffin-NBP2-49321-img0010.jpg "Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP2-49321] - Staining of human skeletal muscle using Anti-CLIC4 antibody NBP2-49321.")
![Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP2-49321] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunohistochemistry-Paraffin-NBP2-49321-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP2-49321] - Staining in human kidney and skeletal muscle tissues using anti-CLIC4 antibody. Corresponding CLIC4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunohistochemistry-Paraffin-NBP2-49321-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP2-49321] - Staining of human colon, endometrium, kidney and skeletal muscle using Anti-CLIC4 antibody NBP2-49321 (A) shows similar protein distribution across tissues to independent antibody NBP1-85574 (B).](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunohistochemistry-Paraffin-NBP2-49321-img0006.jpg)
![Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP2-49321] - Staining of human colon.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunohistochemistry-Paraffin-NBP2-49321-img0007.jpg)
![Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP2-49321] - Staining of human endometrium.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunohistochemistry-Paraffin-NBP2-49321-img0008.jpg)
![Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP2-49321] - Staining of human kidney using Anti-CLIC4 antibody NBP2-49321.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunohistochemistry-Paraffin-NBP2-49321-img0009.jpg)
![Western Blot: CLIC4 Antibody [NBP1-33474] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CLIC4 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Western-Blot-NBP1-33474-img0004.jpg "Western Blot: CLIC4 Antibody [NBP1-33474] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CLIC4 antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: CLIC4 Antibody [NBP1-33474] - Paraformaldehyde-fixed HeLa, using CLIC4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33474-img0002.jpg)
![Western Blot: CLIC4 Antibody [NBP1-33474] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Western-Blot-NBP1-33474-img0003.jpg)
![Immunohistochemistry: CLIC4 Antibody [NBP1-33474] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using CLIC4 antibody (NBP1-33474) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp1-33474_rabbit-polyclonal-clic4-antibody-91020242063384.jpg)
![Western Blot: CLIC4 Antibody [NBP1-33474] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CLIC4 antibody (NBP1-33474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33474_rabbit-polyclonal-clic4-antibody-910202420104446.jpg)
![Western Blot: CLIC4 Antibody (SR1481) [NBP3-22596] - Western blot analysis using NBP3-22596 in 293 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22596_rabbit-clic4-mab-sr1481-672023106272.jpg "Western Blot: CLIC4 Antibody (SR1481) [NBP3-22596] - Western blot analysis using NBP3-22596 in 293 cell lysate.")
![Western Blot: CLIC4 Antibody (JE65-09) [NBP3-32192] - Western blot analysis of CLIC4 on different lysates with Rabbit anti-CLIC4 antibody (NBP3-32192) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: JAR cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: RAW264.7 cell lysate (20 ug/Lane) Lane 5: Neuro-2a cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Human kidney tissue lysate (40 ug/Lane) Lane 8: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32192) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32192_rabbit-clic4-mab-je65-09-278202416285255.jpg "Western Blot: CLIC4 Antibody (JE65-09) [NBP3-32192] - Western blot analysis of CLIC4 on different lysates with Rabbit anti-CLIC4 antibody (NBP3-32192) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: JAR cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: RAW264.7 cell lysate (20 ug/Lane) Lane 5: Neuro-2a cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Human kidney tissue lysate (40 ug/Lane) Lane 8: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32192) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: CLIC4 Antibody (JE65-09) [NBP3-32192] - Immunocytochemistry analysis of HeLa cells labeling CLIC4 with Rabbit anti-CLIC4 antibody (NBP3-32192) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CLIC4 antibody (NBP3-32192) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32192_rabbit-clic4-mab-je65-09-2920241114835.jpg)
![Flow Cytometry: CLIC4 Antibody (JE65-09) [NBP3-32192] - Flow cytometric analysis of HeLa cells labeling CLIC4. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32192, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32192_rabbit-clic4-mab-je65-09-29202416372180.jpg)
![Western Blot:[NBP3-30037]-Detection of human and mouse CLIC4 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLIC4 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30037_rabbit-clic4-pab-23120251159500.jpg "Western Blot:[NBP3-30037]-Detection of human and mouse CLIC4 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLIC4 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation:CLIC4 AntibodyNBP3-30622] -Detection of human CLIC4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CLIC4 antibody NBP3-30622 (lot NBP3-30622-1) used for IP at 6 µg per reaction. CLIC4 was also immunoprecipitated by rabbit anti-CLIC4.. For blotting immunoprecipitated CLIC4, NBP3-30622 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30622_rabbit-clic4-pab-301220241316306.jpg "Immunoprecipitation:CLIC4 AntibodyNBP3-30622] -Detection of human CLIC4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CLIC4 antibody NBP3-30622 (lot NBP3-30622-1) used for IP at 6 µg per reaction. CLIC4 was also immunoprecipitated by rabbit anti-CLIC4.. For blotting immunoprecipitated CLIC4, NBP3-30622 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: CLIC4 Antibody (6O0W5) [NBP3-33286] - Western blot analysis of various lysates, using CLIC4 Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-33286_rabbit-clic4-mab-6o0w5-1012202411404097.jpg "Western Blot: CLIC4 Antibody (6O0W5) [NBP3-33286] - Western blot analysis of various lysates, using CLIC4 Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![SDS-Page: Recombinant Human CLIC4 Protein [NBP1-51032] - 15 % SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-CLIC4-Protein-SDS-Page-NBP1-51032-img0001.jpg "SDS-Page: Recombinant Human CLIC4 Protein [NBP1-51032] - 15 % SDS-PAGE (3ug)")
![Western Blot: CLIC4 Overexpression Lysate (Adult Normal) [NBL1-09269] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CLIC4.](http://images.novusbio.com/fullsize/CLIC4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09269-img0002.jpg "Western Blot: CLIC4 Overexpression Lysate (Adult Normal) [NBL1-09269] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CLIC4.")