HAF007). A specific band was detected for FKBP38 at approximately 60 to 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat FKBP38 Monoclonal Antibody (Catalog # MAB3580) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FKBP38 at approximately 60 to 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3580-SP | |||
HAF109). A specific band was detected for FKBP38 at approximately 60 - 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, MDA-MB-468 human breast cancer cell line, A549 human lung carcinoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat FKBP38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3580) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for FKBP38 at approximately 60 - 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3580-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22302-50ul | |||
CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the nucleus of epithelial cells in intestinal glands. View our protocol for 
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
![Western Blot: FKBP38 Antibody (2J7P6) [NBP3-16684] - Western blot analysis of extracts of various cell lines, using FKBP38 Rabbit mAb (NBP3-16684) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/FKBP38-Antibody-2J7P6-Western-Blot-NBP3-16684-img0003.jpg "Western Blot: FKBP38 Antibody (2J7P6) [NBP3-16684] - Western blot analysis of extracts of various cell lines, using FKBP38 Rabbit mAb (NBP3-16684) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: FKBP38 Antibody (2J7P6) [NBP3-16684] - Western blot analysis of extracts of various cell lines, using FKBP38 Rabbit mAb (NBP3-16684) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/FKBP38-Antibody-2J7P6-Western-Blot-NBP3-16684-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: FKBP38 Antibody (2J7P6) [NBP3-16684] - Confocal imaging of NIH/3T3 cells using FKBP38 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16684_rabbit-fkbp38-mab-2j7p6-8220252010492.jpg)
![Western Blot: FKBP38 Antibody [NBP1-77909] - Shows detection of exogenous FKBP8 in 50 ug of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4C, overnight. Personal Communication, Olga Aprelikova, CCR-NCI, Bethesda, MD.](http://images.novusbio.com/fullsize/FKBP38-Antibody-Western-Blot-NBP1-77909-img0001.jpg "Western Blot: FKBP38 Antibody [NBP1-77909] - Shows detection of exogenous FKBP8 in 50 ug of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4C, overnight. Personal Communication, Olga Aprelikova, CCR-NCI, Bethesda, MD.")
![Western Blot: FKBP38 Antibody [NBP1-59018] - Human Brain lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/FKBP38-Antibody-Western-Blot-NBP1-59018-img0003.jpg "Western Blot: FKBP38 Antibody [NBP1-59018] - Human Brain lysate, concentration 0.2-1 ug/ml.")
![Immunohistochemistry: FKBP38 Antibody [NBP1-59018] - Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue Observed Staining: Cytoplasm in hepatocytes Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec](http://images.novusbio.com/fullsize/FKBP38-Antibody-Immunohistochemistry-NBP1-59018-img0004.jpg)
![Western Blot: FKBP38 Antibody [NB110-85535] - Analysis of exogenous FKBP8 in 50 ug of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4 C, overnight.](http://images.novusbio.com/fullsize/FKBP38-Antibody-Western-Blot-NB110-85535-img0005.jpg "Western Blot: FKBP38 Antibody [NB110-85535] - Analysis of exogenous FKBP8 in 50 ug of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4 C, overnight.")
![Western Blot: FKBP38 Antibody [NB110-85535] - protein A purified anti-FKBP8 antibody shows detection of exogenous FKBP8 in 50 ug of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4C, overnight.](http://images.novusbio.com/fullsize/FKBP38-Antibody-Western-Blot-NB110-85535-img0002.jpg)
![Western Blot: FKBP38 Antibody (7-2#) [NBP3-41692] - Sample: Recombinant FKBP38, Human.](http://images.novusbio.com/fullsize/nbp3-41692_mouse-fkbp38-mab-7-2-6122024212795.jpg "Western Blot: FKBP38 Antibody (7-2#) [NBP3-41692] - Sample: Recombinant FKBP38, Human.")
![Western Blot: FKBP38 Antibody (SR1916) [NBP3-22302] - Western blot analysis using NBP3-22302 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/nbp3-22302_rabbit-fkbp38-mab-sr1916-57202315521831.jpg "Western Blot: FKBP38 Antibody (SR1916) [NBP3-22302] - Western blot analysis using NBP3-22302 in Jurkat cell lysate.")
![Western Blot: FKBP38 Antibody [NBP1-45571] - Analysis of FKBP-38 with mouse intestine tissue lysate (left lane) and Jurkat cell lysate (Right lane).](http://images.novusbio.com/fullsize/FKBP38-Antibody-Western-Blot-NBP1-45571-img0002.jpg "Western Blot: FKBP38 Antibody [NBP1-45571] - Analysis of FKBP-38 with mouse intestine tissue lysate (left lane) and Jurkat cell lysate (Right lane).")
![Western Blot: FKBP38 Antibody [NBP1-59019] - Hela cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/FKBP38-Antibody-Western-Blot-NBP1-59019-img0002.jpg "Western Blot: FKBP38 Antibody [NBP1-59019] - Hela cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: FKBP38 Overexpression Lysate (Adult Normal) [NBP2-10362] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FKBP38.](http://images.novusbio.com/fullsize/FKBP38-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10362-img0001.jpg "Western Blot: FKBP38 Overexpression Lysate (Adult Normal) [NBP2-10362] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FKBP38.")
