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HAF016). A specific band was detected for CD2AP at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">
HAF016). A specific band was detected for CD2AP at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, Ramos human Burkitt's lymphoma cell line, KG-1 human acute myelogenous leukemia cell line, M1 mouse myeloid leukemia cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse/Rat CD2AP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4474) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD2AP at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />