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![Western Blot: FGFR3 Antibody (HL1595) - Azide and BSA Free [NBP3-25465] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FGFR3 antibody [HL1595] (NBP3-25465) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25465_rabbit-fgfr3-mab-hl1595-azide-and-bsa-free-212024939196.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: FGFR3 Antibody (HL1595) - Azide and BSA Free [NBP3-25465] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FGFR3 antibody [HL1595] (NBP3-25465) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: FGFR3 Antibody (HL1595) - Azide and BSA Free [NBP3-25465] - Wild-type (WT) and FGFR3 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FGFR3 antibody [HL1595] (NBP3-25465) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25465_rabbit-fgfr3-mab-hl1595-azide-and-bsa-free-2120249351316.jpg)
![Western Blot: FGFR3 Antibody (HL1595) - Azide and BSA Free [NBP3-25465] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FGFR3 antibody [HL1595] (NBP3-25465) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25465_rabbit-fgfr3-mab-hl1595-azide-and-bsa-free-2120249425410.jpg)
![Western Blot: FGFR3 Antibody [NB600-1509] - Western blot analysis of apoptotic markers following 24 and 48 hrs of selenite (5 uM), GS-Se-SG (5 uM) and Se-DL-cystine (100 uM) treatment. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/full/10.1111/jcmm.12214), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FGFR3-Antibody-Western-Blot-NB600-1509-img0001.jpg "Western Blot: FGFR3 Antibody [NB600-1509] - Western blot analysis of apoptotic markers following 24 and 48 hrs of selenite (5 uM), GS-Se-SG (5 uM) and Se-DL-cystine (100 uM) treatment. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/full/10.1111/jcmm.12214), licensed under a CC-BY license.")
![Western Blot: FGFR3 Antibody [NB600-1509] - (A) Western blot analysis of apoptotic markers following 24 & 48 hrs of selenite (5 μM), GS-Se-SG (5 μM) & Se-DL-cystine (100 μM) treatment. (B) Western blot analysis of autophagic & ER stress markers, evaluated after 1, 3, 6 & 18 hrs of treatment with selenite (5 μM), GS-Se-SG (5 μM) & Se-DL-cystine (100 μM). (C) Acridine orange staining of cells after 12 hrs of treatment with selenite (5 μM), GS-Se-SG (5 μM) & Se-DL-cystine (100 μM) (magnification 40 ×). (D) High-resolution transmission electron microscopy micrographs of control & 24 hrs Se-DL-cystine-treated cells, showing the presence of autophagosome, as evident from double-layered membrane structure with inclusion bodies. (E) Western blot detection of ubiquitinylated proteins after treatments with different selenium compounds (similar doses as previous) for 6 hrs. Five microgram cell lysate was loaded into each lane. The lane intensity was analysed by using Adobe Photoshop program (version CS5). beta -actin was used as a loading control (Fig. 4A & B). C: Control; 1: selenite; 2: GS-Se-SG; 3: Se-DL-cystine. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24400844), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1509_rabbit-polyclonal-fgfr3-antibody-310202416175253.jpg)
![Western Blot: FGFR3 [p Tyr 724] Antibody (SR2305) [NBP3-21595] - Western blot analysis using NBP3-21595 in (1) MCF7 treated with pervanadate cell lysate; (2) untreated.](http://images.novusbio.com/fullsize/nbp3-21595_rabbit-fgfr3-p-tyr-724-mab-sr2305-5720231624734.jpg "Western Blot: FGFR3 [p Tyr 724] Antibody (SR2305) [NBP3-21595] - Western blot analysis using NBP3-21595 in (1) MCF7 treated with pervanadate cell lysate; (2) untreated.")
![Western Blot: FGF R3 Antibody (1E3F9) [NBP2-52469] - Analysis using FGFR3 mAb against human FGFR3 (AA: 529-694) recombinant protein. (Expected MW is 44.1 kDa)](http://images.novusbio.com/fullsize/FGF-R3-Antibody-1E3F9-Western-Blot-NBP2-52469-img0001.jpg "Western Blot: FGF R3 Antibody (1E3F9) [NBP2-52469] - Analysis using FGFR3 mAb against human FGFR3 (AA: 529-694) recombinant protein. (Expected MW is 44.1 kDa)")
![Western Blot: FGF R3 Antibody (1E3F9) [NBP2-52469] - Analysis using FGFR3 mAb against HEK293 (1) and FGFR3 (AA: 529-694)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/FGF-R3-Antibody-1E3F9-Western-Blot-NBP2-52469-img0002.jpg)
![ELISA: FGFR3 Antibody (1E3F9) [NBP2-52469] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/FGFR3-Antibody-1E3F9-ELISA-NBP2-52469-img0003.jpg)
![Western Blot: FGFR3 Overexpression Lysate (Adult Normal) [NBP2-09054] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FGFR3.](http://images.novusbio.com/fullsize/FGFR3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09054-img0001.jpg "Western Blot: FGFR3 Overexpression Lysate (Adult Normal) [NBP2-09054] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FGFR3.")