HAF109). A specific band was detected for eEF-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NRK rat normal kidney cell line, U2OS human osteosarcoma cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat eEF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3806) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eEF-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3806-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57792-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21831-50ul | |||
![Western Blot: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Western blot analysis of extracts of various cell lines, using eEF-2 Rabbit mAb (NBP3-16866) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/eEF-2-Antibody-4E2Z5-Western-Blot-NBP3-16866-img0005.jpg "Western Blot: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Western blot analysis of extracts of various cell lines, using eEF-2 Rabbit mAb (NBP3-16866) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Western blot analysis of extracts of Rat spleen, using eEF-2 Rabbit mAb (NBP3-16866) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/eEF-2-Antibody-4E2Z5-Western-Blot-NBP3-16866-img0006.jpg)
![Immunohistochemistry-Paraffin: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Immunohistochemistry of paraffin-embedded human appendix using eEF-2 Rabbit mAb (NBP3-16866) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/eEF-2-Antibody-4E2Z5-Immunohistochemistry-Paraffin-NBP3-16866-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Immunofluorescence analysis of NIH-3T3 cells using eEF-2 Rabbit mAb (NBP3-16866) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/eEF-2-Antibody-4E2Z5-Immunocytochemistry-Immunofluorescence-NBP3-16866-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Immunofluorescence analysis of C6 cells using eEF-2 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16866_rabbit-eef-2-mab-4e2z5-5220251215475.jpg)
![Immunocytochemistry/ Immunofluorescence: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Immunofluorescence analysis of U-2 OS cells using eEF-2 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16866_rabbit-eef-2-mab-4e2z5-5220251215481.jpg)
![Western Blot: eEF-2 Antibody [NBP2-57792] - Analysis using Anti-EEF2 antibody NBP2-57792 (A) shows similar pattern to independent antibody NBP2-56755 (B).](http://images.novusbio.com/fullsize/eEF-2-Antibody-Western-Blot-NBP2-57792-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: eEF-2 Antibody [NBP2-57792] - Analysis using Anti-EEF2 antibody NBP2-57792 (A) shows similar pattern to independent antibody NBP2-56755 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: eEF-2 Antibody [NBP2-57792] - Staining of human cell line HEK 293 shows localization to plasma membrane, cytosol & aggresome.](http://images.novusbio.com/fullsize/eEF-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57792-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: eEF-2 Antibody [NBP2-57792] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/eEF-2-Antibody-Western-Blot-NBP2-57792-img0005.jpg)
![Western Blot: eEF-2 Antibody [NB100-87020] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibody used at 0.04 ug/ml.](http://images.novusbio.com/fullsize/eEF-2-Antibody-Western-Blot-NB100-87020-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: eEF-2 Antibody [NB100-87020] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibody used at 0.04 ug/ml.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: eEF-2 Antibody [NB100-87020] - Section of human breast carcinoma (left) and mouse teratoma (right).Antibody: Affinity purified rabbit anti-eEF2 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/eEF-2-Antibody-Immunohistochemistry-Paraffin-NB100-87020-img0006.jpg)
![Western Blot: eEF-2 Antibody (JE32-57) [NBP3-32293] - Western blot analysis of eEF-2 on different lysates with Rabbit anti-eEF-2 antibody (NBP3-32293) at 1/1,000 dilution.<br/><br/> Lane 1: HeLa cell lysate (20 ug/Lane)<br/> Lane 2: Jurkat cell lysate (20 ug/Lane)<br/> Lane 3: NIH/3T3 cell lysate (20 ug/Lane)<br/> Lane 4: PC-12 cell lysate (20 ug/Lane)<br/> Lane 5: Mouse liver tissue lysate (40 ug/Lane)<br/> Lane 6: Rat spleen tissue lysate (40 ug/Lane)<br/><br/> Predicted band size: 95 kDa<br/> Observed band size: 95 kDa<br/><br/> Exposure time: 8 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32293) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32293_rabbit-eef-2-mab-je32-57-278202416285245.jpg "Western Blot: eEF-2 Antibody (JE32-57) [NBP3-32293] - Western blot analysis of eEF-2 on different lysates with Rabbit anti-eEF-2 antibody (NBP3-32293) at 1/1,000 dilution.<br/><br/> Lane 1: HeLa cell lysate (20 ug/Lane)<br/> Lane 2: Jurkat cell lysate (20 ug/Lane)<br/> Lane 3: NIH/3T3 cell lysate (20 ug/Lane)<br/> Lane 4: PC-12 cell lysate (20 ug/Lane)<br/> Lane 5: Mouse liver tissue lysate (40 ug/Lane)<br/> Lane 6: Rat spleen tissue lysate (40 ug/Lane)<br/><br/> Predicted band size: 95 kDa<br/> Observed band size: 95 kDa<br/><br/> Exposure time: 8 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32293) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: eEF-2 Antibody (JE32-57) [NBP3-32293] - Immunocytochemistry analysis of HeLa cells labeling eEF-2 with Rabbit anti-eEF-2 antibody (NBP3-32293) at 1/250 dilution.<br/><br/> Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-eEF-2 antibody (NBP3-32293) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32293_rabbit-eef-2-mab-je32-57-29202410334013.jpg)
![Flow Cytometry: eEF-2 Antibody (JE32-57) [NBP3-32293] - Flow cytometric analysis of HeLa cells labeling eEF-2.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32293, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32293_rabbit-eef-2-mab-je32-57-29202416453236.jpg)
![Western Blot: eEF-2 Antibody (SR2299) [NBP3-21831] - Western blot analysis using NBP3-21831 in (1) A431 cell lysate; (2) NIH/3T3 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21831_rabbit-eef-2-mab-sr2299-57202317215191.jpg "Western Blot: eEF-2 Antibody (SR2299) [NBP3-21831] - Western blot analysis using NBP3-21831 in (1) A431 cell lysate; (2) NIH/3T3 cell lysate.")
![Western Blot: eEF-2 Antibody [NBP2-56755] - Analysis using Anti-EEF2 antibody NBP2-56755 (A) shows similar pattern to independent antibody NBP2-57792 (B).](http://images.novusbio.com/fullsize/eEF-2-Antibody-Western-Blot-NBP2-56755-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: eEF-2 Antibody [NBP2-56755] - Analysis using Anti-EEF2 antibody NBP2-56755 (A) shows similar pattern to independent antibody NBP2-57792 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: eEF-2 Antibody [NBP2-56755] - Immunohistochemical staining of human ovary shows stroma positivity.](http://images.novusbio.com/fullsize/eEF-2-Antibody-Immunohistochemistry-NBP2-56755-img0003.jpg)
![Western Blot: eEF-2 Antibody [NBP1-54383] - Titration: 0.2-1 ug/ml, Positive Control: Hela cell lysate.](http://images.novusbio.com/fullsize/eEF-2-Antibody-Western-Blot-NBP1-54383-img0006.jpg "Western Blot: eEF-2 Antibody [NBP1-54383] - Titration: 0.2-1 ug/ml, Positive Control: Hela cell lysate.")
![Immunohistochemistry-Paraffin: eEF-2 Antibody [NBP1-54383] - Human small intestine tissue at an antibody concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/eEF-2-Antibody-Immunohistochemistry-Paraffin-NBP1-54383-img0005.jpg)
![Western Blot: eEF-2 Antibody [NBP2-16274] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/eEF-2-Antibody-Western-Blot-NBP2-16274-img0002.jpg "Western Blot: eEF-2 Antibody [NBP2-16274] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: EEF2 Overexpression Lysate (Adult Normal) [NBP2-08160] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EEF2.](http://images.novusbio.com/fullsize/EEF2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08160-img0001.jpg "Western Blot: EEF2 Overexpression Lysate (Adult Normal) [NBP2-08160] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EEF2.")
![SDS-Page: Recombinant Human eEF-2 Protein [NBP2-51776] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize/EEF2K-Recombinant-Protein-SDS-Page-NBP2-51776-img0001.jpg "SDS-Page: Recombinant Human eEF-2 Protein [NBP2-51776] - 15% SDS Page (3 ug)")
