| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7934-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70569 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7934-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21772-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59464-50ul | |||
![Western Blot: Dihydrofolate Reductase/DHFR Antibody (2B10) [H00001719-M01] - Folic acid treatment restores endothelial specific DHFR expression and activity in Ang II-infused apoE null mice.DHFR expression (A, n?=?8-11) and activity (B, n?=?5-7) measured from endothelial cells (ECs) and EC-denuded aortas isolated from apoE null mice after 4 weeks of infusion. Data show that DHFR expression and activity was restored in FA treated animals specifically in the endothelial cells. eNOS was stained to show that isolation/removal of ECs from the vessels was successful. *p<0.05. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0088899) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-2B10-Western-Blot-H00001719-M01-img0017.jpg "Western Blot: Dihydrofolate Reductase/DHFR Antibody (2B10) [H00001719-M01] - Folic acid treatment restores endothelial specific DHFR expression and activity in Ang II-infused apoE null mice.DHFR expression (A, n?=?8-11) and activity (B, n?=?5-7) measured from endothelial cells (ECs) and EC-denuded aortas isolated from apoE null mice after 4 weeks of infusion. Data show that DHFR expression and activity was restored in FA treated animals specifically in the endothelial cells. eNOS was stained to show that isolation/removal of ECs from the vessels was successful. *p<0.05. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0088899) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (2B10) [H00001719-M01] - Analysis of monoclonal antibody to DHFR on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-2B10-Immunocytochemistry-Immunofluorescence-H00001719-M01-img0012.jpg)
![Western Blot: Dihydrofolate Reductase/DHFR Antibody (2B10) [H00001719-M01] - DHFR monoclonal antibody (M01), clone 2B10 Analysis of DHFR expression in HeLa.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-2B10-Western-Blot-H00001719-M01-img0014.jpg)
![Western Blot: Dihydrofolate Reductase/DHFR Antibody (2B10) [H00001719-M01] - DHFR monoclonal antibody (M01), clone 2B10. Analysis of DHFR expression in PC-12.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-2B10-Western-Blot-H00001719-M01-img0015.jpg)
![Western Blot: Dihydrofolate Reductase/DHFR Antibody (2B10) [H00001719-M01] - Analysis of DHFR expression in transfected 293T cell line by DHFR monoclonal antibody (M01), clone 2B10.Lane 1: DHFR transfected lysate(21.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-2B10-Western-Blot-H00001719-M01-img0016.jpg)
![Sandwich ELISA: Dihydrofolate Reductase/DHFR Antibody (2B10) [H00001719-M01] - Detection limit for recombinant GST tagged DHFR is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-2B10-Sandwich-ELISA-H00001719-M01-img0013.jpg)
![Western Blot: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Dihydrofolate reductase monoclonal antibody.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-6G7-Western-Blot-NBP1-47727-img0014.jpg "Western Blot: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Dihydrofolate reductase monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Dihydrofolate reductase.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-6G7-Immunocytochemistry-Immunofluorescence-NBP1-47727-img0008.jpg)
![Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-Dihydrofolate reductase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-6G7-Immunohistochemistry-Paraffin-NBP1-47727-img0012.jpg)
![Western Blot: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Dihydrofolate reductase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Dihydrofolate reductase.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-6G7-Western-Blot-NBP1-47727-img0013.jpg)
![Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Paraffin-embedded Adenocarcinoma of ovary tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50)](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-6G7-Immunohistochemistry-Paraffin-NBP1-47727-img0009.jpg)
![Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Paraffin-embedded Carcinoma of thyroid tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50)](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-6G7-Immunohistochemistry-Paraffin-NBP1-47727-img0010.jpg)
![Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Dihydrofolate reductase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-6G7-Immunohistochemistry-Paraffin-NBP1-47727-img0011.jpg)
![Western Blot: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Left: lysate of glioblastoma xenograft. Non-treated. Right: methotrexate-treated lysate of glioblastoma xenograft. 10 ug each protein were loaded.Antibody dilution is 1/3000 in 2% BSA TBST. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-47727_mouse-dihydrofolate-reductase-dhfr-mab-oti6g7-western-blot-67202311401..jpg)
![Western Blot: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Dihydrofolate reductase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with ant](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-OTI6G7-Azide-and-BSA-Free-Western-Blot-NBP2-70569-img0002.jpg "Western Blot: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Dihydrofolate reductase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with ant")
![Immunocytochemistry/Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Dihydrofolate reductase.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-OTI6G7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70569-img0003.jpg)
![Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Dihydrofolate reductase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-OTI6G7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70569-img0006.jpg)
![Western Blot: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Dihydrofolate reductase monoclonal antibody.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-OTI6G7-Azide-and-BSA-Free-Western-Blot-NBP2-70569-img0001.jpg)
![Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Paraffin-embedded Adenocarcinoma of ovary tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50)](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-OTI6G7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70569-img0004.jpg)
![Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Paraffin-embedded Carcinoma of thyroid tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50)](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-OTI6G7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70569-img0005.jpg)
![Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-Dihydrofolate reductase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-OTI6G7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70569-img0007.jpg)
![Western Blot: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163] - A. 30 ug PC-12 whole cell lysate/extract 12 % SDS-PAGE DHFR antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-Western-Blot-NBP2-16163-img0005.jpg "Western Blot: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163] - A. 30 ug PC-12 whole cell lysate/extract 12 % SDS-PAGE DHFR antibody dilution: 1:1000")
![Immunocytochemistry/Immunofluorescence: Dihydrofolate reductase Antibody [NBP2-16163] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Dihydrofolate-reductase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16163-img0003.jpg)
![Immunohistochemistry-Paraffin: Dihydrofolate reductase Antibody [NBP2-16163] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Dihydrofolate-reductase-Antibody-Immunohistochemistry-Paraffin-NBP2-16163-img0002.jpg)
![Immunoprecipitation: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163] - Immunoprecipitation of DHFR protein from 293T whole cell extracts using 5 ug of DHFR antibody. Western blot analysis was performed using DHFR antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-Immunoprecipitation-NBP2-16163-img0008.jpg)
![Western Blot: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Dihydrofolate Reductase/DHFR antibody (NBP2-16163) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-16163_rabbit-polyclonal-dihydrofolate-reductase-dhfr-antibody-910202420122097.jpg)
![Western Blot: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195] - Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, Rat heart tissue, Mouse kidney tissue, Mouse liver tissue. All lanes: Dihydrofolate Reductase/DHFR antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 22, 16 KDa. Observed band size: 22 KDa.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-9B2-Western-Blot-NBP3-15195-img0004.jpg "Western Blot: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195] - Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, Rat heart tissue, Mouse kidney tissue, Mouse liver tissue. All lanes: Dihydrofolate Reductase/DHFR antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 22, 16 KDa. Observed band size: 22 KDa.")
![Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195] - IHC image of Dihydrofolate Reductase/DHFR antibody diluted at 1:100 and staining in paraffin-embedded human liver cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-9B2-Immunohistochemistry-NBP3-15195-img0003.jpg)
![Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195] - IHC image of Dihydrofolate Reductase/DHFR antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-9B2-Immunohistochemistry-NBP3-15195-img0002.jpg)
![Immunoprecipitation: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195] - Immunoprecipitating Dihydrofolate Reductase/DHFR in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of Dihydrofolate Reductase/DHFR antibody in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: Dihydrofolate Reductase/DHFR antibody (2ug)+ Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-9B2-Immunoprecipitation-NBP3-15195-img0001.jpg)
![Western Blot: Dihydrofolate Reductase/DHFR Antibody (SR1325) [NBP3-21772] - Western blot analysis using NBP3-21772 in (1) HeLa cell lysate; (2) Mouse spleen lysate.](http://images.novusbio.com/fullsize/nbp3-21772_rabbit-dihydrofolate-reductase-dhfr-mab-sr1325-57202316112191.jpg "Western Blot: Dihydrofolate Reductase/DHFR Antibody (SR1325) [NBP3-21772] - Western blot analysis using NBP3-21772 in (1) HeLa cell lysate; (2) Mouse spleen lysate.")
![Western Blot: Dihydrofolate Reductase/DHFR Antibody (JE31-05) [NBP3-32268] - Western blot analysis of Dihydrofolate Reductase/DHFR on different lysates with Rabbit anti-Dihydrofolate Reductase/DHFR antibody (NBP3-32268) at 1/1,000 dilution. Lane 1: HCT116 cell lysate (15 ug/Lane) Lane 2: HeLa cell lysate (15 ug/Lane) Lane 3: U-2 OS cell lysate (15 ug/Lane) Lane 4: K-562 cell lysate (15 ug/Lane) Lane 5: MCF7 cell lysate (15 ug/Lane) Lane 6: HEK-293 cell lysate (15 ug/Lane) Lane 7: Jurkat cell lysate (15 ug/Lane) Lane 8: HepG2 cell lysate (15 ug/Lane) Lane 9: LO2 cell lysate (15 ug/Lane) Lane 10: Mouse liver tissue lysate (30 ug/Lane) Lane 11: Rat liver tissue lysate (30 ug/Lane) Predicted band size: 21.5 kDa Observed band size: 21.5 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32268) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32268_rabbit-dihydrofolate-reductase-dhfr-mab-je31-05-278202416331265.jpg "Western Blot: Dihydrofolate Reductase/DHFR Antibody (JE31-05) [NBP3-32268] - Western blot analysis of Dihydrofolate Reductase/DHFR on different lysates with Rabbit anti-Dihydrofolate Reductase/DHFR antibody (NBP3-32268) at 1/1,000 dilution. Lane 1: HCT116 cell lysate (15 ug/Lane) Lane 2: HeLa cell lysate (15 ug/Lane) Lane 3: U-2 OS cell lysate (15 ug/Lane) Lane 4: K-562 cell lysate (15 ug/Lane) Lane 5: MCF7 cell lysate (15 ug/Lane) Lane 6: HEK-293 cell lysate (15 ug/Lane) Lane 7: Jurkat cell lysate (15 ug/Lane) Lane 8: HepG2 cell lysate (15 ug/Lane) Lane 9: LO2 cell lysate (15 ug/Lane) Lane 10: Mouse liver tissue lysate (30 ug/Lane) Lane 11: Rat liver tissue lysate (30 ug/Lane) Predicted band size: 21.5 kDa Observed band size: 21.5 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32268) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (JE31-05) [NBP3-32268] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Dihydrofolate Reductase/DHFR antibody (NBP3-32268) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32268) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32268_rabbit-dihydrofolate-reductase-dhfr-mab-je31-05-298202413585558.jpg)
![Western Blot: Dihydrofolate Reductase/DHFR Antibody (5B2) [NBP2-59464] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DHFR antibody (1:1000). Proteins werevisualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : Jurkat cell lysate Lane 3 : 293T cell lysate](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-5B2-Western-Blot-NBP2-59464-img0007.jpg "Western Blot: Dihydrofolate Reductase/DHFR Antibody (5B2) [NBP2-59464] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DHFR antibody (1:1000). Proteins werevisualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : Jurkat cell lysate Lane 3 : 293T cell lysate")
![Immunocytochemistry/Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (5B2) [NBP2-59464] - Analysis of DHFR in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human DHFR antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-5B2-Immunocytochemistry-Immunofluorescence-NBP2-59464-img0006.jpg)
![Western Blot: Dihydrofolate Reductase/DHFR Antibody (7N3X4) [NBP3-16805] - Western blot analysis of extracts of various cell lines, using Dihydrofolate Reductase/DHFR Rabbit mAb (NBP3-16805) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-7N3X4-Western-Blot-NBP3-16805-img0001.jpg "Western Blot: Dihydrofolate Reductase/DHFR Antibody (7N3X4) [NBP3-16805] - Western blot analysis of extracts of various cell lines, using Dihydrofolate Reductase/DHFR Rabbit mAb (NBP3-16805) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")