NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="RGM-C/Hemojuvelin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human/Mouse RGM-C/Hemojuvelin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3720) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3720-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3634-SP | |||
DY990). Mouse Anti-Human RGM-C/Hemojuvelin Monoclonal Antibody (Catalog # MAB37201) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human RGM-C/Hemojuvelin protein was serially diluted 2-fold and captured by Mouse Anti-Human RGM-C/Hemojuvelin Monoclonal Antibody (Catalog # MAB3720) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human RGM-C/Hemojuvelin Monoclonal Antibody (Catalog # MAB37201) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3720-SP | |||
MAB3720) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human RGM-C/Hemojuvelin Monoclonal Antibody (Catalog # MAB37201) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human RGM-C/Hemojuvelin protein was serially diluted 2-fold and captured by Mouse Anti-Human RGM-C/Hemojuvelin Monoclonal Antibody (Catalog # MAB3720) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human RGM-C/Hemojuvelin Monoclonal Antibody (Catalog # MAB37201) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB37201-SP | |||
![N/A RGM-C/Hemojuvelin [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_RGMC_MRGMC0_ELISA_1025.jpg "N/A RGM-C/Hemojuvelin [HRP]")
![N/A RGM-C/Hemojuvelin [HRP]](http://images.novusbio.com/fullsize/elisa/RGMC_MRGMC0_ELISA_462.jpg)
![Immunohistochemistry-Paraffin: RGM-C/Hemojuvelin Antibody (1C12) [H00148738-M01] - Analysis of monoclonal antibody to HFE2 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/RGM-C-Hemojuvelin-Antibody-1C12-Immunohistochemistry-Paraffin-H00148738-M01-img0002.jpg "Immunohistochemistry-Paraffin: RGM-C/Hemojuvelin Antibody (1C12) [H00148738-M01] - Analysis of monoclonal antibody to HFE2 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.")
![Sandwich ELISA: RGM-C/Hemojuvelin Antibody (1C12) [H00148738-M01] - Detection limit for recombinant GST tagged HFE2 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RGM-C-Hemojuvelin-Antibody-1C12-Sandwich-ELISA-H00148738-M01-img0003.jpg)
![N/A RGM-C/Hemojuvelin [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_RGMC_DY372005_ELISA_2439.jpg "N/A RGM-C/Hemojuvelin [Biotin]")
![Western Blot: Repulsive Guidance Molecule C Overexpression Lysate (Adult Normal) [NBL1-11525] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Repulsive Guidance Molecule C.](http://images.novusbio.com/fullsize/Repulsive-Guidance-Molecule-C-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11525-img0002.jpg "Western Blot: Repulsive Guidance Molecule C Overexpression Lysate (Adult Normal) [NBL1-11525] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Repulsive Guidance Molecule C.")
![Western Blot: RGM-C/Hemojuvelin Antibody [NBP1-79531] - Titration: 0.2-1 ug/ml, Positive Control: HT1080 cell lysate.](http://images.novusbio.com/fullsize/RGM-C-Hemojuvelin-Antibody-Western-Blot-NBP1-79531-img0003.jpg "Western Blot: RGM-C/Hemojuvelin Antibody [NBP1-79531] - Titration: 0.2-1 ug/ml, Positive Control: HT1080 cell lysate.")
