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Products Related to Gene-ID: 1482

32 Results for "1482"
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AF2444

NKX2.5 Antibody [Unconjugated]

(2 Reviews)
(14 Publications)
100 μg
AF2444
EOMES programs hESCs into functional CMs at high efficiency. a Illustration of growth factor-mediated and optimized EOMES induction-based cardiac differentiation protocols. Bottom right: Immunoblot validating doxycycline-dependent EOMES expression in a transgenic EOMESKO/E.TET-ON hESC line. b Typical yields of hESC-CMs (left, flow cytometry) and NKX2.5 expression (right, immunoblot) obtained with the two protocols (day 10). c Immunostainings 21 days after the initiation of EOMES induction. Weak perinuclear ANP staining is typical in overall MLC2v-positive hESC-CMs. Scale bars: 25 (top) and 50 µm (bottom). d Acceleration and slowdown of spontaneous beat rates in pCMs following exposure to 10 µM isoprenaline and 10 µM propranolol, respectively, on multielectrode arrays. e Microarray-based time course analysis comparing the indicated protocols and cell lines. RESCUE cells carry an inducible EOMES transgene on EOMESKO HuES6 background. Underlying data are from Supplementary Data 2 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29382828), licensed under a CC-BY license. Not internally tested by R&D Systems.
Hu
Species Glossary
Reactivity: Hu
WB, IHC
Applications: WB, IHC
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 AF2444U-100UG
Alexa Fluor 405 AF2444V-100UG
Alexa Fluor 488 AF2444G-100UG
Alexa Fluor 532 AF2444X-100UG
Alexa Fluor 594 AF2444T-100UG
Alexa Fluor 647 AF2444R-100UG
Alexa Fluor 700 AF2444N-100UG
Alexa Fluor 750 AF2444S-100UG
Allophycocyanin AF2444A
Biotin BAF2444
CoraFluor 1 AF2444CL1
DyLight 350 AF2444D
DyLight 405 AF2444E
DyLight 488 AF2444K
DyLight 550 AF2444L
DyLight 594 AF2444M
DyLight 650 AF2444W
DyLight 680 AF2444Y
DyLight 755 AF2444Z
FITC AF2444F
HRP AF2444H
Janelia Fluor 525 AF2444JF525
Janelia Fluor 549 AF2444I
Janelia Fluor 646 AF2444J
Janelia Fluor 669 AF2444JF669
PE AF2444P
PerCP AF2444C
mFluor Violet 450 SE AF2444MFV450
mFluor Violet 500 SE AF2444MFV500
mFluor Violet 610 SE AF2444MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF2444-SP
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MAB2444

NKX2.5 Antibody (259416)

(10 Publications)
100 μg
MAB2444
NKX2.5 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human NKX2.5 Monoclonal Antibody (Catalog # MAB2444) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Hu
Species Glossary
Reactivity: Hu
IHC
Applications: IHC
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB2444U-100UG
Alexa Fluor 405 FAB2444V-100UG
Alexa Fluor 488 FAB2444G-100UG
Alexa Fluor 532 FAB2444X-100UG
Alexa Fluor 594 FAB2444T-100UG
Alexa Fluor 647 FAB2444R-100UG
Alexa Fluor 700 FAB2444N-100UG
Alexa Fluor 750 FAB2444S-100UG
Allophycocyanin FAB2444A
Biotin FAB2444B
CoraFluor 1 FAB2444CL1
DyLight 350 FAB2444D
DyLight 405 FAB2444E
DyLight 488 FAB2444K
DyLight 550 FAB2444L
DyLight 594 FAB2444M
DyLight 650 FAB2444W
DyLight 680 FAB2444Y
DyLight 755 FAB2444Z
FITC FAB2444F
HRP FAB2444H
Janelia Fluor 525 FAB2444JF525
Janelia Fluor 549 FAB2444I
Janelia Fluor 646 FAB2444J
Janelia Fluor 669 FAB2444JF669
PE FAB2444P
PerCP FAB2444C
mFluor Violet 450 SE FAB2444MFV450
mFluor Violet 500 SE FAB2444MFV500
mFluor Violet 610 SE FAB2444MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB2444-SP
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NBP1-31558

NKX2.5 Antibody - BSA Free

(2 Publications)
100 ul
NBP1-31558
Immunocytochemistry/Immunofluorescence: NKX2.5 Antibody [NBP1-31558] - Paraformaldehyde-fixed mouse ESC D3, using antibody at 1:200 dilution.
Hu, Mu, Rt, Bv, Ca
Species Glossary
Reactivity: Hu, Mu, Rt, Bv, Ca
WB, Flow, ICC/IF, IHC
Applications: WB, Flow, ICC/IF, IHC
Rabbit Polyclonal
Host: Rabbit Polyclonal
Unconjugated  
         
Validated by:          
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NBP2-24675

NKX2.5 Antibody

0.1 mg
NBP2-24675
Western Blot: Nkx2.5 Antibody [NBP2-24675] - Western blot analysis of Nkx2.5 in human heart lysate using 1) prebleed and 2) NBP2-24675 at 1 ug/ml.
Hu, Pm, Po, Bv, Pm, Eq, Fe, RM
Species Glossary
Reactivity: Hu, Pm, Po, Bv, Pm, Eq, Fe, RM
WB, IHC
Applications: WB, IHC
Rabbit Polyclonal
Host: Rabbit Polyclonal
IMG-6493A
Alt Catalog# IMG-6493A
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
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NBP1-51953

NKX2.5 Antibody

(2 Publications)
0.1 mg
NBP1-51953
Western Blot: NKX2.5 Antibody [NBP1-51953] - (1ug/ml) staining of Human (A), Mouse (B) and (2ug/ml) Rat (C) Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Hu, Mu, Rt, Bv, Ca
Species Glossary
Reactivity: Hu, Mu, Rt, Bv, Ca
WB, ELISA, IHC
Applications: WB, ELISA, IHC
Goat Polyclonal
Host: Goat Polyclonal
Unconjugated  
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MAB24441

NKX2.5 Antibody (259415) [Unconjugated]

100 μg
MAB24441
Western blot shows lysates of CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Human NKX2.5 Monoclonal Antibody (Catalog # MAB24441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NKX2.5 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, Simple Western
Applications: WB, Simple Western
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB24441U-100UG
Alexa Fluor 405 FAB24441V-100UG
Alexa Fluor 488 FAB24441G-100UG
Alexa Fluor 532 FAB24441X-100UG
Alexa Fluor 594 FAB24441T-100UG
Alexa Fluor 647 FAB24441R-100UG
Alexa Fluor 700 FAB24441N-100UG
Alexa Fluor 750 FAB24441S-100UG
Allophycocyanin FAB24441A
Biotin FAB24441B
CoraFluor 1 FAB24441CL1
DyLight 350 FAB24441D
DyLight 405 FAB24441E
DyLight 488 FAB24441K
DyLight 550 FAB24441L
DyLight 594 FAB24441M
DyLight 650 FAB24441W
DyLight 680 FAB24441Y
DyLight 755 FAB24441Z
FITC FAB24441F
HRP FAB24441H
Janelia Fluor 525 FAB24441JF525
Janelia Fluor 549 FAB24441I
Janelia Fluor 646 FAB24441J
Janelia Fluor 669 FAB24441JF669
PE FAB24441P
PerCP FAB24441C
mFluor Violet 450 SE FAB24441MFV450
mFluor Violet 500 SE FAB24441MFV500
mFluor Violet 610 SE FAB24441MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB24441-SP
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NBL1-13659

NKX2.5 Overexpression Lysate

0.1 mg
NBL1-13659
Western Blot: Nkx2.5 Overexpression Lysate (Adult Normal) [NBL1-13659] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nkx2.5.
Hu
Species Glossary
Reactivity: Hu
WB
Applications: WB
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BAF2444

NKX2.5 Antibody [Biotin]

50 μg
BAF2444
NKX2.5 was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human NKX2.5 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2444) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Hu
Species Glossary
Reactivity: Hu
WB, IHC
Applications: WB, IHC
Goat Polyclonal
Host: Goat Polyclonal
Biotin  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF2444
Carrier Free AF2444-SP
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AF2444V

NKX2.5 Antibody [Alexa Fluor® 405]

100 μg
Hu
Species Glossary
Reactivity: Hu
WB, IHC
Applications: WB, IHC
Alexa Fluor 405  
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AF2444T

NKX2.5 Antibody [Alexa Fluor® 594]

100 μg
Hu
Species Glossary
Reactivity: Hu
WB, IHC
Applications: WB, IHC
Alexa Fluor 594  
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AF2444G

NKX2.5 Antibody [Alexa Fluor® 488]

100 μg
Hu
Species Glossary
Reactivity: Hu
WB, IHC
Applications: WB, IHC
Alexa Fluor 488  
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AF2444X

NKX2.5 Antibody [Alexa Fluor® 532]

100 μg
Hu
Species Glossary
Reactivity: Hu
WB, IHC
Applications: WB, IHC
Alexa Fluor 532  
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