| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35347-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35650-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99686-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57977-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37929-20ul | |||
![Western Blot: CKII alpha prime polypeptide Antibody [NB100-379] - Detection of Human and Mouse CKII alpha' by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha' antibody NB100-379 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-Western-Blot-NB100-379-img0004.jpg "Western Blot: CKII alpha prime polypeptide Antibody [NB100-379] - Detection of Human and Mouse CKII alpha' by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha' antibody NB100-379 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CKII alpha prime polypeptide Antibody [NB100-379] - Detection of human CKII alpha' by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CKII alpha' antibody NB100-379 used for IP at 6 ug per reaction. CKII alpha' was also immunoprecipitated by rabbit anti-CKII alpha' antibody NB100-378. For blotting immunoprecipitated CKII alpha', NB100-379 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-Immunoprecipitation-NB100-379-img0005.jpg)
![Western Blot: CKII alpha prime polypeptide Antibody [NBP3-35347] - Western blot analysis of various lysates using CKII alpha prime polypeptide Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/nbp3-35347_rabbit-ckii-alpha-prime-polypeptide-pab-91220241512133.jpg "Western Blot: CKII alpha prime polypeptide Antibody [NBP3-35347] - Western blot analysis of various lysates using CKII alpha prime polypeptide Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunohistochemistry: CKII alpha prime polypeptide Antibody [NBP3-35347] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35347_rabbit-ckii-alpha-prime-polypeptide-pab-912202414213764.jpg)
![Immunocytochemistry/ Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP3-35347] - Immunofluorescence analysis of NIH-3T3 cells using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35347_rabbit-ckii-alpha-prime-polypeptide-pab-912202415171197.jpg)
![Immunohistochemistry: CKII alpha prime polypeptide Antibody [NBP3-35347] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35347_rabbit-ckii-alpha-prime-polypeptide-pab-912202414163074.jpg)
![Immunohistochemistry: CKII alpha prime polypeptide Antibody [NBP3-35347] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35347_rabbit-ckii-alpha-prime-polypeptide-pab-91220241418158.jpg)
![Immunohistochemistry: CKII alpha prime polypeptide Antibody [NBP3-35347] - Immunohistochemistry analysis of paraffin-embedded Rat lung using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35347_rabbit-ckii-alpha-prime-polypeptide-pab-91220241514367.jpg)
![Western Blot: CKII alpha prime polypeptide Antibody [NBP3-35650] - Western blot analysis of various lysates using CKII alpha prime polypeptide Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35650_rabbit-ckii-alpha-prime-polypeptide-pab-10122024120692.jpg "Western Blot: CKII alpha prime polypeptide Antibody [NBP3-35650] - Western blot analysis of various lysates using CKII alpha prime polypeptide Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: CKII alpha prime polypeptide Antibody [NBP3-35650] - Western blot analysis of lysates from HeLa cells, using CKII alpha prime polypeptide Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35650_rabbit-ckii-alpha-prime-polypeptide-pab-1012202411572658.jpg)
![Immunocytochemistry/ Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP3-35650] - Immunofluorescence analysis of NIH/3T3 cells using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35650_rabbit-ckii-alpha-prime-polypeptide-pab-101220241214447.jpg)
![Immunocytochemistry/ Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP3-35650] - Immunofluorescence analysis of PC-12 cells using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35650_rabbit-ckii-alpha-prime-polypeptide-pab-1012202411572640.jpg)
![Western Blot: CKII alpha prime polypeptide Antibody [NBP2-99686] - Anti-CKII alpha prime polypeptide rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 45 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-Western-Blot-NBP2-99686-img0005.jpg "Western Blot: CKII alpha prime polypeptide Antibody [NBP2-99686] - Anti-CKII alpha prime polypeptide rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 45 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: CKII alpha prime polypeptide Antibody [NBP2-99686] - CKII alpha prime polypeptide was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate4 ul anti-CKII alpha prime polypeptide rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CKII alpha prime polypeptide rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 41 kDa](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-Immunoprecipitation-NBP2-99686-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP2-99686] - Immunofluorescence staining of CKII alpha prime polypeptide in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99686 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-99686_rabbit-polyclonal-ckii-alpha-prime-polypeptide-antibody-23520251664765.png)
![Immunocytochemistry/Immunofluorescence: CKII alpha prime polypeptide Antibody (1E8) [H00001459-M03] - Analysis of monoclonal antibody to CSNK2A2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-1E8-Immunocytochemistry-Immunofluorescence-H00001459-M03-img0002.jpg "Immunocytochemistry/Immunofluorescence: CKII alpha prime polypeptide Antibody (1E8) [H00001459-M03] - Analysis of monoclonal antibody to CSNK2A2 on HeLa cell. Antibody concentration 10 ug/ml")
![Sandwich ELISA: CKII alpha prime polypeptide Antibody (1E8) [H00001459-M03] - Detection limit for recombinant GST tagged CSNK2A2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-1E8-Sandwich-ELISA-H00001459-M03-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP2-57977] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & vesicles.](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57977-img0001.jpg "Immunocytochemistry/Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP2-57977] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & vesicles.")
![Western Blot: CKII alpha prime polypeptide Antibody (3W5P4) [NBP3-16076] - Western blot analysis of various lysates, using CKII alpha prime polypeptide Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16076_rabbit-ckii-alpha-prime-polypeptide-mab-3w5p4-7220251245330.jpg "Western Blot: CKII alpha prime polypeptide Antibody (3W5P4) [NBP3-16076] - Western blot analysis of various lysates, using CKII alpha prime polypeptide Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.")
![Western Blot: CKII alpha prime polypeptide Antibody [NBP1-56690] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-Western-Blot-NBP1-56690-img0002.jpg "Western Blot: CKII alpha prime polypeptide Antibody [NBP1-56690] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.")
![Western Blot: CKII alpha prime polypeptide Antibody [NBP1-56402] - HepG2 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-Western-Blot-NBP1-56402-img0002.jpg "Western Blot: CKII alpha prime polypeptide Antibody [NBP1-56402] - HepG2 cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: CKII alpha prime polypeptide Overexpression Lysate (Adult Normal) [NBL1-09537] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CKII alpha prime polypeptide.](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09537-img0002.jpg "Western Blot: CKII alpha prime polypeptide Overexpression Lysate (Adult Normal) [NBL1-09537] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CKII alpha prime polypeptide.")
![Western Blot: CKII alpha prime polypeptide Antibody [NBP3-37929] - Western blot analysis of various lysates, using CKII alpha prime polypeptide Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-37929_rabbit-ckii-alpha-prime-polypeptide-pab-101220241233818.jpg "Western Blot: CKII alpha prime polypeptide Antibody [NBP3-37929] - Western blot analysis of various lysates, using CKII alpha prime polypeptide Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.")
![Immunocytochemistry/Immunofluorescence: CKII alpha prime polypeptide Antibody (4F2) [H00001459-M01] - Analysis of monoclonal antibody to CSNK2A2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-4F2-Immunocytochemistry-Immunofluorescence-H00001459-M01-img0002.jpg "Immunocytochemistry/Immunofluorescence: CKII alpha prime polypeptide Antibody (4F2) [H00001459-M01] - Analysis of monoclonal antibody to CSNK2A2 on HeLa cell . Antibody concentration 10 ug/ml.")