Products Related to Gene-ID: 1435

216-MC) was serially diluted and captured by Mouse Anti-Human M‑CSF Monoclonal Antibody (Catalog # MAB616) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human M‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF216) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 216-MC) was serially diluted and captured by Mouse Anti-Human M‑CSF Monoclonal Antibody (Catalog # MAB616) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human M‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF216) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human M‑CSF (Catalog # 216-MC) was serially diluted and captured by Mouse Anti-Human M‑CSF Monoclonal Antibody (Catalog # MAB616) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human M‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF216) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

416-ML) stimulates proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse M-CSF (10 ng/mL) is neutralized (green line) by increasing concen­trations of Goat Anti-Mouse M-CSF Polyclonal Antibody (Catalog # AB-416-NA). The ND₅₀ is typically 0.2-0.6 µg/mL." class="big_lightbox"> 416-ML) stimulates proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse M-CSF (10 ng/mL) is neutralized (green line) by increasing concen­trations of Goat Anti-Mouse M-CSF Polyclonal Antibody (Catalog # AB-416-NA). The ND₅₀ is typically 0.2-0.6 µg/mL." alt="Recombinant Mouse M-CSF (Catalog # 416-ML) stimulates proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse M-CSF (10 ng/mL) is neutralized (green line) by increasing concen­trations of Goat Anti-Mouse M-CSF Polyclonal Antibody (Catalog # AB-416-NA). The ND₅₀ is typically 0.2-0.6 µg/mL." class="big_thumb" />

MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." alt="Human peripheral blood mononuclear cells (PBMC) were stained with Mouse Anti-Human CD14 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB3832F) and either (A) Mouse Anti-Human M‑CSF Monoclonal Antibody (Catalog # mab2161) or (B) isotype control antibody (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

329-MR) is immobilized at 0.5 μg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human M‑CSF Protein (Catalog # BT216) that produces 50% of the optimal binding response is 1.50-15.0 ng/mL." class="big_lightbox"> 329-MR) is immobilized at 0.5 μg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human M‑CSF Protein (Catalog # BT216) that produces 50% of the optimal binding response is 1.50-15.0 ng/mL." alt="When Recombinant Human M-CSF R/CD115 Fc Chimera Protein (329-MR) is immobilized at 0.5 μg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human M‑CSF Protein (Catalog # BT216) that produces 50% of the optimal binding response is 1.50-15.0 ng/mL." class="big_thumb" />