DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder." class="big_lightbox">
DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder." alt="EMR-1 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse EMR-1 Monoclonal Antibody (Catalog # MAB11811) at 3ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog #
DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder." class="big_thumb" />
DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder." class="big_lightbox">
DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder." class="big_thumb" />
DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder." class="big_lightbox">
DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder." class="big_thumb" />
DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder." class="big_lightbox">
DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder." class="big_thumb" />
HAF005). A specific band was detected for F4/80 at approximately 130-300 kDa (as indicated). This experiment was conducted under reducing conditions and using
Western Blot Buffer Group 1." class="big_lightbox">
HAF005). A specific band was detected for F4/80 at approximately 130-300 kDa (as indicated). This experiment was conducted under reducing conditions and using
Western Blot Buffer Group 1." class="big_thumb" />
MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113)." alt="RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 Monoclonal Antibody (Catalog # MAB5580, filled histogram) or isotype control antibody (MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113)." class="big_thumb" />
DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="EMR-1 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse EMR-1 Monoclonal Antibody (Catalog # MAB11811) at 3ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: F4/80 Antibody (CI-A3-1) [NB600-404] - Lymph node stained for macrophages, red and B cells CD79b, green; nuclei are stained with DAPI, blue.](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Immunocytochemistry-Immunofluorescence-NB600-404-img0045.jpg)
![Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - A surface stain was performed on RAW 246.7 cells with F4/80 antibody (CI-A3-1) NB600-404AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Flow-Cytometry-NB600-404-img0048.jpg)
![Immunocytochemistry/Immunofluorescence: F4/80 Antibody (CI-A3-1) [NB600-404] - Analysis of monocytes cultured in M-CSF using F4/80 antibody. Primary antibody dilution: 1:200. Overnight incubation in RT. Image from verified customer review.](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Immunocytochemistry-Immunofluorescence-NB600-404-img0053.jpg)
![Flow Cytometry: F4/80 Antibody (CI-A3-1) - BSA Free [NB600-404] - Surface staining of F4/80 in CT26 colorectal carcinoma tumor model. Using Allophycocyanin conjugated version of the antibody (NB600-404APC). Image from verified customer review](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-BSA-Free-Flow-Cytometry-NB600-404-img0054.jpg)
![Immunohistochemistry-Frozen: F4/80 Antibody (CI-A3-1) [NB600-404] - Frozen mouse spleen with F4/80 anti-mouse antibody.](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Immunohistochemistry-Frozen-NB600-404-img0046.jpg)
![Immunohistochemistry-Frozen: F4/80 Antibody (CI-A3-1) [NB600-404] - Mouse spleen stained with A: Rat anti Mouse. B: CI-A3-1 clone preincubated with 2 molar excess of human anti Idiotypic.](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Immunohistochemistry-Frozen-NB600-404-img0047.jpg)
![Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of J774 cells with F4/80 antibody.](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Flow-Cytometry-NB600-404-img0037.jpg)
![Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of mouse peritoneal macrophages with clone CI-A3-1 F4/80 antibody.](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Flow-Cytometry-NB600-404-img0043.jpg)
![Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404]](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Flow-Cytometry-NB600-404-img0049.jpg)
![Immunohistochemistry: F4/80 Antibody (Cl:A3-1) [NB600-404] - Lymphocyte interaction inside the DC harboring scaffoldsDC scaffolds (beDC) or scaffolds only, placed in tumor bearing mice or DC-scaffold placed in normal mice were recovered two weeks post-implantation & processed for immunohistochemistry to demonstrate various cell types. (A) Relative size of the biomatrices harvested from various mice groups as depicted in the figure (far left panel). n = 3–5. The dotted line in ‘gross specimen’ differentiates the biomatrix from the reactive host-tissue. Representative images of biomatrix sections stained by the respective antibodies as indicated in the figure. The bar in the ‘gross structure’ is equivalent to 1 mm, whereas for other panels is equal to 100 μm. (B) Snapshots from Supplementary Movie S2 showing lymphocyte (green) movement & interaction with DCs (red) inside the biomatrix. The start point of the observation was set as the zero time point. (C) Lymphocyte division (white arrows) inside the biomatrix in the vicinity of DC (red). Representative snapshot from Supplementary Movie S3. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27223090), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-404_rat-monoclonal-f4-80-antibody-ci-a3-1-310202416171463.jpg)
NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm." class="big_thumb" />
![Flow Cytometry: F4/80 Antibody (BM8) [NBP1-60140] - Splenocytes isolated from a IL1R-/- mouse (IL1b receptor KO) were stained with and CD45-e450 (1:100 in FACS buffer) for 30 minutes. Cells were then washed and analyzed by flow cytometry. Left panel shows the isotype control. This image was submitted via a customer review. Image from the PE version of this antibody.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Flow-Cytometry-NBP1-60140-img0006.jpg "Flow Cytometry: F4/80 Antibody (BM8) [NBP1-60140] - Splenocytes isolated from a IL1R-/- mouse (IL1b receptor KO) were stained with and CD45-e450 (1:100 in FACS buffer) for 30 minutes. Cells were then washed and analyzed by flow cytometry. Left panel shows the isotype control. This image was submitted via a customer review. Image from the PE version of this antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Frozen: F4/80 Antibody (BM8) [NBP1-60140] - Red: F4/80, Green: CD31/PECAM-1, Blue: DAPI+ nuclei. Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with PE-conjugated F4/80 and Alex647-conjugated CD31/PECAM-1 antibody (MEC 7.46) for 2 hours at the room temperature. This image was submitted via a customer review. Image from the PE version of this antibody.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Immunohistochemistry-Frozen-NBP1-60140-img0007.jpg)
![Immunohistochemistry: F4/80 Antibody (BM8) [NBP1-60140] - Expression on macrophages in mouse liver. Staining of frozen tissue section with antibody BM8.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Immunohistochemistry-NBP1-60140-img0003.jpg)
![Flow Cytometry: F4/80 Antibody (BM8) [NBP1-60140] - Analysis using the FITC conjugate of NBP1-60140. Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 FITC.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Flow-Cytometry-NBP1-60140-img0005.jpg)
DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # 
HAF005). A specific band was detected for F4/80 at approximately 130-300 kDa (as indicated). This experiment was conducted under reducing conditions and using 
VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (
VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for ![Immunohistochemistry: F4/80 Antibody (4J7L6) [NBP3-33457] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using F4/80 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33457_rabbit-f4-80-mab-4j7l6-1012202411334071.jpg "Immunohistochemistry: F4/80 Antibody (4J7L6) [NBP3-33457] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using F4/80 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: F4/80 Antibody (4J7L6) [NBP3-33457] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using F4/80 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33457_rabbit-f4-80-mab-4j7l6-1012202411353088.jpg)
![Immunohistochemistry: F4/80 Antibody (4J7L6) [NBP3-33457] - Immunohistochemistry analysis of paraffin-embedded mouse spleen using F4/80 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-33457_rabbit-f4-80-mab-4j7l6-52202511392258.jpg)
![Western Blot: F4/80 Antibody (PSH0-85) [NBP3-32323] - Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (NBP3-32323) at 1/1,000 dilution.<br/><br/> Lane 1: RAW264.7 cell lysate (no heat) (20 ug/Lane)<br/> Lane 2: L929 cell lysate (no heat) (negative) (20 ug/Lane)<br/> Lane 3: Rat spleen tissue lysate (70 heat) (40 ug/Lane)<br/><br/> Predicted band size: 102 kDa<br/> Observed band size: 160 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32323) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32323_rabbit-f4-80-mab-psh0-85-27820241631634.jpg "Western Blot: F4/80 Antibody (PSH0-85) [NBP3-32323] - Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (NBP3-32323) at 1/1,000 dilution.<br/><br/> Lane 1: RAW264.7 cell lysate (no heat) (20 ug/Lane)<br/> Lane 2: L929 cell lysate (no heat) (negative) (20 ug/Lane)<br/> Lane 3: Rat spleen tissue lysate (70 heat) (40 ug/Lane)<br/><br/> Predicted band size: 102 kDa<br/> Observed band size: 160 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32323) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: F4/80 Antibody (PSH0-85) [NBP3-32323] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-F4/80 antibody (NBP3-32323) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32323) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32323_rabbit-f4-80-mab-psh0-85-298202414262680.jpg)
![Immunohistochemistry: F4/80 Antibody (PSH0-85) [NBP3-32323] - Immunofluorescence analysis of paraffin-embedded mouse liver tissue labeling F4/80 with Rabbit anti-F4/80 antibody (NBP3-32323) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32323, green) at 1/500 dilution overnight at 4 ℃, washed with PBS.<br/><br/> Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32323_rabbit-f4-80-mab-psh0-85-298202414161820.jpg)
![Western blot analysis on different lysates with NBP3-32323 at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate (no heat) (20 µg/Lane) Lane 2: L929 cell lysate (no heat) (negative) (20 µg/Lane) Lane 3: Rat spleen tissue lysate (70℃ heat) (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 102 kDa Observed band size: 160 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32323 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32323_-western-blot-639204597797257410.jpg)
![Western Blot: F4/80 Antibody (PSH01-87) [NBP3-32324] - Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (NBP3-32324) at 1/1,000 dilution.<br/><br/> Lane 1: RAW264.7(no heat) cell lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 102 kDa<br/> Observed band size: 160 kDa<br/><br/> Exposure time: 5 minutes;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32324) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32324_rabbit-f4-80-mab-psh01-87-278202415295528.jpg "Western Blot: F4/80 Antibody (PSH01-87) [NBP3-32324] - Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (NBP3-32324) at 1/1,000 dilution.<br/><br/> Lane 1: RAW264.7(no heat) cell lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 102 kDa<br/> Observed band size: 160 kDa<br/><br/> Exposure time: 5 minutes;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32324) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: F4/80 Antibody (PSH01-87) [NBP3-32324] - Immunofluorescence analysis of paraffin-embedded mouse liver tissue labeling F4/80 with Rabbit anti-F4/80 antibody (NBP3-32324) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32324, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32324_rabbit-f4-80-mab-psh01-87-29820241435214.jpg)
![Immunohistochemistry: F4/80 Antibody (PSH01-87) [NBP3-32324] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-F4/80 antibody (NBP3-32324) at 1/5,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32324) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32324_rabbit-f4-80-mab-psh01-87-298202414161825.jpg)
![Western blot analysis on different lysates with NBP3-32324 at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate (no heat) (20 µg/Lane) Lane 2: L-929 cell lysate (no heat) (negative) (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 102 kDa Observed band size: 160 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32324 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32324_-western-blot-639204595247368316.jpg)
![Flow Cytometry: F4/80 Antibody (CI:A3-1) [NBP2-61613] - Figure 3 BMDMs were stained with Fc Silent recombinant anti-F4/80 [Cl:A3-1] or isotype control (Fc Silent anti-fluorescein [4-4-20 (enhanced)] IgG2b. These were fluorescently labelled using the secondary antibody, goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor 647(AF647) commercially available from a competitor. The Fc silent format however overcomes this issue as seen in Figure 3, where the Fc silent recombinant anti-F4/80 yields a strong and distinct signal, whilst the isotype control shows no discernible difference to the background staining from the secondary antibody alone. Therefore, with Fc Silent reagents, no Fc-blocking products are required. [Data courtesy of Lewis Taylor.]](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Flow-Cytometry-NBP2-61613-img0003.jpg "Flow Cytometry: F4/80 Antibody (CI:A3-1) [NBP2-61613] - Figure 3 BMDMs were stained with Fc Silent recombinant anti-F4/80 [Cl:A3-1] or isotype control (Fc Silent anti-fluorescein [4-4-20 (enhanced)] IgG2b. These were fluorescently labelled using the secondary antibody, goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor 647(AF647) commercially available from a competitor. The Fc silent format however overcomes this issue as seen in Figure 3, where the Fc silent recombinant anti-F4/80 yields a strong and distinct signal, whilst the isotype control shows no discernible difference to the background staining from the secondary antibody alone. Therefore, with Fc Silent reagents, no Fc-blocking products are required. [Data courtesy of Lewis Taylor.]")
