CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to vascular endothelial cells within glomeruli. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="Podocan was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse Podocan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3104) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to vascular endothelial cells within glomeruli. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3104-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93238-0.02ml | |||
![Western Blot: Podocan Antibody [NBP2-19881] - Sample (30 ug of whole cell lysate) A: 293T 7. 5% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/Podocan-Antibody-Western-Blot-NBP2-19881-img0001.jpg "Western Blot: Podocan Antibody [NBP2-19881] - Sample (30 ug of whole cell lysate) A: 293T 7. 5% SDS PAGE gel, diluted at 1:2000.")
![Immunohistochemistry-Paraffin: Podocan Antibody [NBP2-19881] - Human gastric cancer, using PODN antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Podocan-Antibody-Immunohistochemistry-Paraffin-NBP2-19881-img0002.jpg)
![Western Blot: Podocan Antibody [NBP2-93238] - Analysis of extracts of various cell lines, using Podocan at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.](http://images.novusbio.com/fullsize/Podocan-Antibody-Western-Blot-NBP2-93238-img0001.jpg "Western Blot: Podocan Antibody [NBP2-93238] - Analysis of extracts of various cell lines, using Podocan at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.")
![Western Blot: Podocan Overexpression Lysate (Adult Normal) [NBL1-14560] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Podocan.](http://images.novusbio.com/fullsize/Podocan-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14560-img0002.jpg "Western Blot: Podocan Overexpression Lysate (Adult Normal) [NBL1-14560] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Podocan.")
![Western Blot: Podocan Antibody [H00127435-B01P] - Analysis of PODN expression in transfected 293T cell line by PODN polyclonal antibody. Lane 1: PODN transfected lysate(72.71 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Podocan-Antibody-Western-Blot-H00127435-B01P-img0001.jpg "Western Blot: Podocan Antibody [H00127435-B01P] - Analysis of PODN expression in transfected 293T cell line by PODN polyclonal antibody. Lane 1: PODN transfected lysate(72.71 KDa). Lane 2: Non-transfected lysate.")
