HAF008). A specific band was detected for Acetyl Histone H4 (Lys5) at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 10mM Sodium (Na) Butyrate for 24 hours. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Acetyl Histone H4 (Lys5) Monoclonal Antibody (Catalog # MAB9549) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Acetyl Histone H4 (Lys5) at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB9549-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16137-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26013 | |||
NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for ![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys13, ac Lys5, ac Lys8] Antibody [NBP2-59253] - Mouse NIH3T3 cells were stained with the antibody against H4K5,8,12,16ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4K5,8,12,16ac antibody (left) diluted 1:500 in blocking solution followed by an anti- rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys13--ac-Lys5--ac-Lys8]-Antibody-Immunofluorescence-NBP2-59253-img0003.jpg "Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys13, ac Lys5, ac Lys8] Antibody [NBP2-59253] - Mouse NIH3T3 cells were stained with the antibody against H4K5,8,12,16ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4K5,8,12,16ac antibody (left) diluted 1:500 in blocking solution followed by an anti- rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.")
![Chromatin Immunoprecipitation: Histone H4 [ac Lys13, ac Lys5, ac Lys8] Antibody [NBP2-59253] - ChIP assays were performed using human HeLa cells, the antibody against H4K5,8,12,16ac and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP- seq kit on sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH gene, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys13--ac-Lys5--ac-Lys8]-Antibody-Chromatin-Immunoprecipitation-NBP2-59253-img0002.jpg)
![ELISA: Histone H4 [ac Lys13, ac Lys5, ac Lys8] Antibody [NBP2-59253] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H4K5,8,12,16ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:21,200.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys13--ac-Lys5--ac-Lys8]-Antibody-ELISA-NBP2-59253-img0001.jpg)
![Dot Blot: Histone H4 [ac Lys13, ac Lys5, ac Lys8] Antibody [NBP2-59253] - One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Thr figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys13-ac-Lys5-ac-Lys8]-Antibody-Dot-Blot-NBP2-59253-img0004.jpg)
![Western Blot: Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free [NBP3-16137] - Western blot analysis of lysates from NIH/3T3 cells, using Histone H4 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by Sodium lactate(100mM) for 24h.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Enhanced Kit.<br>Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-16137_rabbit-histone-h4-lactyl-lys-5-pab-52202511392278.jpg "Western Blot: Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free [NBP3-16137] - Western blot analysis of lysates from NIH/3T3 cells, using Histone H4 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by Sodium lactate(100mM) for 24h.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Enhanced Kit.<br>Exposure time: 60s.")
![Western Blot: Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free [NBP3-16137] - Western blot analysis of lysates from C6 cells, using Histone H4 Rabbit pAb at 1:1000 dilution. C6 cells were treated by Sodium lactate(100mM) for 24h.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Enhanced Kit.<br>Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-16137_rabbit-histone-h4-lactyl-lys-5-pab-52202511374158.jpg)
![Western Blot: Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free [NBP3-16137] - Western blot analysis of lysates from MCF7 cells, using Histone H4 Rabbit pAb at 1:1000 dilution. MCF7 cells were treated by Sodium lactate(100mM) for 24h.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Enhanced Kit.<br>Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-16137_rabbit-histone-h4-lactyl-lys-5-pab-52202511365166.jpg)
![Dot Blot: Histone H4 [Lactyl Lys5] Antibody [NBP3-16137] - Dot-blot analysis of all sorts of peptides using Histone H4 antibody (NBP3-16137) at 1:4000 dilution.](http://images.novusbio.com/fullsize/Histone-H4-[Lactyl-Lys5]-Antibody-Dot-Blot-NBP3-16137-img0002.jpg)
![Western Blot: Histone H4 Antibody (4R4N3) [NBP3-33209] - Western blot analysis of various lysates using Histone H4 Rabbit mAb at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-33209_rabbit-histone-h4-mab-4r4n3-91220241519515.jpg "Western Blot: Histone H4 Antibody (4R4N3) [NBP3-33209] - Western blot analysis of various lysates using Histone H4 Rabbit mAb at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 10s.")
![Immunohistochemistry: Histone H4 Antibody (4R4N3) [NBP3-33209] - Immunohistochemistry analysis of paraffin-embedded Human colon using Histone H4 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33209_rabbit-histone-h4-mab-4r4n3-9122024159577.jpg)
![Immunohistochemistry: Histone H4 Antibody (4R4N3) [NBP3-33209] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Histone H4 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33209_rabbit-histone-h4-mab-4r4n3-912202415175289.jpg)
![Immunohistochemistry: Histone H4 Antibody (4R4N3) [NBP3-33209] - Immunohistochemistry analysis of paraffin-embedded Rat liver using Histone H4 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33209_rabbit-histone-h4-mab-4r4n3-912202415161817.jpg)
![Western Blot: Histone H4 [Dimethyl Arg3] Antibody [NBP2-59265] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4R3me2(sym). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Arg3]-Antibody-Western-Blot-NBP2-59265-img0001.jpg "Western Blot: Histone H4 [Dimethyl Arg3] Antibody [NBP2-59265] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4R3me2(sym). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Dimethyl Arg3] Antibody [NBP2-59265] - HeLa cells were stained with the antibody against H4R3me2(sym) and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4R3me2(sym) antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Arg3]-Antibody-Immunofluorescence-NBP2-59265-img0004.jpg)
![Dot Blot: Histone H4 [Dimethyl Arg3] Antibody [NBP2-59265] - To test the cross reactivity of the antibody against H4R3me2(sym), a Dot Blot analysis was performed with peptides containing other histone arginine methylations and the unmodified H4R3. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:5,000. Figure shows the antibody is specific for the H4R3 symmetric dimethylation and also recognizes the H2AR3 symmetric dimethylation.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Arg3]-Antibody-Dot-Blot-NBP2-59265-img0002.jpg)
![ELISA: Histone H4 [Dimethyl Arg3] Antibody [NBP2-59265] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H4R3me2(sym) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:11,750.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Arg3]-Antibody-ELISA-NBP2-59265-img0003.jpg)
![Western Blot: Histone H4 [Methyl Lys20] Antibody [NBP2-59258] - Western blot was performed on whole cell extracts (25 ug, lane 1) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using the antibody against H4K20me1. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein is indicated on the right.](http://images.novusbio.com/fullsize/Histone-H4-[Methyl-Lys20]-Antibody-Western-Blot-NBP2-59258-img0001.jpg "Western Blot: Histone H4 [Methyl Lys20] Antibody [NBP2-59258] - Western blot was performed on whole cell extracts (25 ug, lane 1) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using the antibody against H4K20me1. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein is indicated on the right.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Methyl Lys20] Antibody [NBP2-59258] - HeLa cells were stained with the antibody against H4K20me1 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4K20me1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H4-[Methyl-Lys20]-Antibody-Immunofluorescence-NBP2-59258-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Methyl Lys20] Antibody [NBP2-59258] - ChIP assays were performed using human HeLa cells, the antibody against H4K20me1 and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit on sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the coding region of the active GAPDH and ACTB genes, used as positive controls, and for the GAPDH promoter and a region located 1 kb upstream of the GAPDH promoter, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-[Methyl-Lys20]-Antibody-Chromatin-Immunoprecipitation-NBP2-59258-img0003.jpg)
![ELISA: Histone H4 [Methyl Lys20] Antibody [NBP2-59258] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against H4K20me1 in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:51,100.](http://images.novusbio.com/fullsize/Histone-H4-[Methyl-Lys20]-Antibody-ELISA-NBP2-59258-img0002.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody (1851) [NBP2-42861] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [1851] dilution: 1:5000](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-1851-Western-Blot-NBP2-42861-img0002.jpg "Western Blot: Histone H4 [Dimethyl Lys20] Antibody (1851) [NBP2-42861] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [1851] dilution: 1:5000")
![Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody (1851) [NBP2-42861] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control mouse IgG or anti-Histone H4K20me2 (dimethyl Lys20) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-1851-Chromatin-Immunoprecipitation-NBP2-42861-img0001.jpg)
![Histone H4 (di-Methyl Lys20) antibody [GT1851] detects Histone H4 (di-Methyl Lys20) protein at nucleus by immunofluorescent analysis.<br>Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.<br>Green: Histone H4 (di-Methyl Lys20) protein stained by Histone H4 (di-Methyl Lys20) antibody [GT1851] (NBP2-42861) diluted at 1:500.<br>Blue: Hoechst 33342 staining.<br>](http://images.novusbio.com/fullsize/nbp2-42861_mouse-monoclonal-histone-h4-dimethyl-lys20-antibody-1851-immunocytochemistry-immunofluorescence-261120251962714.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [282] dilution: 1:5000](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-282-Western-Blot-NBP2-42860-img0002.jpg "Western Blot: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [282] dilution: 1:5000")
![Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control mouse IgG or anti-Histone H4K20me2 (dimethyl Lys20) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-282-Chromatin-Immunoprecipitation-ChIP-NBP2-42860-img0001.jpg)
![Histone H4 (di-Methyl Lys20) antibody [GT282] detects Histone H4 (di-Methyl Lys20) protein at nucleus by immunofluorescent analysis.<br>Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.<br>Green: Histone H4 (di-Methyl Lys20) protein stained by Histone H4 (di-Methyl Lys20) antibody [GT282] (NBP2-42860) diluted at 1:500.<br>Blue: Hoechst 33342 staining.<br>](http://images.novusbio.com/fullsize/nbp2-42860_mouse-monoclonal-histone-h4-dimethyl-lys20-antibody-282-immunocytochemistry-immunofluorescence-26112025198364.jpg)
![Western Blot: Histone H4 [ac Lys20] Antibody (RM205) [NBP2-61559] - Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using NBP2-61559, showed a band of Histone H4 acetylated at Lysine 20 in HeLa cells.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys20-Antibody-RM205-Western-Blot-NBP2-61559-img0010.jpg "Western Blot: Histone H4 [ac Lys20] Antibody (RM205) [NBP2-61559] - Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using NBP2-61559, showed a band of Histone H4 acetylated at Lysine 20 in HeLa cells.")
![Multiplex Immunoassay: Histone H4 [ac Lys20] Antibody (RM205) [NBP2-61559] - NBP2-61559 specifically reacts to Histone H4 acetylated at Lysine 20 (K20ac). No cross reactivity with unmodified Lysine 20 (K20 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys20-Antibody-RM205-Multiplex-Immunoassay-NBP2-61559-img0011.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free [NBP2-61559] - Immunocytochemical staining of HeLa cells treated with sodium butyrate, using Clone RM205 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61559_rabbit-monoclonal-histone-h4-ac-lys20-antibody-rm205-immunocytochemistry-immunofluorescence-189202523381714.png)
![Other Histone H4 [Monomethyl Arg3] Antibody Blocking Peptide](http://images.novusbio.com/fullsize/Histone-H4-[Monomethyl-Arg3]-Peptide-Chromatin-Immunoprecipitation-NB21-2011PEP-img0002.jpg "Other Histone H4 [Monomethyl Arg3] Antibody Blocking Peptide")
![Other Histone H4 [Dimethyl Lys20] Antibody Blocking Peptide](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Peptide-Chromatin-Immunoprecipitation-NB21-2089PEP-img0002.jpg "Other Histone H4 [Dimethyl Lys20] Antibody Blocking Peptide")
![Other Histone H4 [Trimethyl Lys20] Antibody Blocking Peptide](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Peptide-Chromatin-Immunoprecipitation-NB21-2090PEP-img0002.jpg "Other Histone H4 [Trimethyl Lys20] Antibody Blocking Peptide")
