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![Western Blot: Histone H4 Antibody [NBP2-42856] - Various tissue extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Western-Blot-NBP2-42856-img0008.jpg "Western Blot: Histone H4 Antibody [NBP2-42856] - Various tissue extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Histone H4 Antibody [NBP2-42856] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 protein stained by Histone H4 antibody diluted at 1:5000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:2000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42856-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H4 Antibody [NBP2-42856] - Rat brain stem. Histone H4 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Immunohistochemistry-Paraffin-NBP2-42856-img0004.jpg)
![Western Blot: Histone H4 Antibody [NBP2-42856] - Analysis of various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody diluted at a dilution of 1:5000.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Western-Blot-NBP2-42856-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H4 Antibody [NBP2-42856] - Mouse fore brain. Histone H4 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Immunohistochemistry-Paraffin-NBP2-42856-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H4 Antibody [NBP2-42856] - Mouse fore brain. Histone H4 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Immunohistochemistry-Paraffin-NBP2-42856-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H4 Antibody [NBP2-42856] - Mouse muscle. Histone H4 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Immunohistochemistry-Paraffin-NBP2-42856-img0003.jpg)
![Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (tri methyl Lys20) antibody dilution: 1:2000](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Western-Blot-NBP2-42862-img0007.jpg "Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (tri methyl Lys20) antibody dilution: 1:2000")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (tri methyl Lys20) protein stained by Histone H4 (tri methyl Lys20) antibody diluted at 1:500. Red: alpha Tubulin antibody [114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42862-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of rat fore brain. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunohistochemistry-Paraffin-NBP2-42862-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of mouse duodenum. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunohistochemistry-Paraffin-NBP2-42862-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of mouse liver. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunohistochemistry-Paraffin-NBP2-42862-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of mouse muscle. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunohistochemistry-Paraffin-NBP2-42862-img0005.jpg)
![Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using Histone H4 [Trimethyl Lys20] Antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Western-Blot-NBP2-59259-img0006.jpg "Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using Histone H4 [Trimethyl Lys20] Antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - (Top): Human osteosarcoma (U2OS) cells were stained with and DAPI. Cells were fixed with ice cold methanol for 10' and blocked with PBS/TX-100 containing 5% normal goat serum. Figure 6A: cells were immunofluorescently labeled with (left) diluted 1:300 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa568 or with DAPI (right), which specifically labels DNA. (Bottom): Staining of the cells with the after incubation of the antibody with blocking peptide, concentration: 5 ng/ul.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59259-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - ChIP assays were performed using undifferentiated human teratocarcinoma cells (NCCIT), the antibody against H4K20me3 and optimized PCR primer sets for qPCR. Sheared chromatin from 10,000 cells was used per ChIP experiment. The antibody was tested at two different dilutions of 1:500 and 1:100. The pre-immune serum (PI) was used as a negative control. Quantitative PCR was performed using primer sets for the satellite repeat Sat2 as a positive control and for the promoter of the house keeping gene c-fos, as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H4K20me3 is preferably present at heterochromatin.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Chromatin-Immunoprecipitation-NBP2-59259-img0004.jpg)
![Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - Histone (acid) extracts of NB4 cells were analysed by Western blot using the Diagenode antibody against H4K20me3, diluted 1:750 in TBS-Tween containing 5% skimmed milk. The location of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Western-Blot-NBP2-59259-img0002.jpg)
![Dot Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - Dot Blot was used to check the specificity of the antibody against H4K20me3 with peptides containing other modifications of histone H3 and H4. Other histone modifications include monoand dimethylation of the same lysine and mono-, di- and trimethylation of lysines 9, 27 and 36 of H3. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:1,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Dot-Blot-NBP2-59259-img0001.jpg)
![ELISA: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against H4K20me3. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:700.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-ELISA-NBP2-59259-img0003.jpg)
![Dot Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - A Dot Blot analysis was performed to test the cross reactivity of Histone H4 [Trimethyl Lys20] Antibody with peptides containing other histone modifications and the unmodified H4K20. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Dot-Blot-NBP2-59259-img0007.jpg)
![Western Blot: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of Un-treated (-) and treated (+, 0.4uM Trichostatin A treatment for 18hr) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 (acetyl Lys16) antibody.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Western-Blot-NBP2-42858-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of Un-treated (-) and treated (+, 0.4uM Trichostatin A treatment for 18hr) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 (acetyl Lys16) antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (acetyl Lys16) protein stained by Histone H4 (acetyl Lys16) antibody [1271] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Immunocytochemistry-Immunofluorescence-NBP2-42858-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Rat kidney. Histone H4K16ac (Acetyl Lys16) stained by Histone H4K16ac (Acetyl Lys16) antibody [1271] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Immunohistochemistry-Paraffin-NBP2-42858-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of mouse brain. Histone H4 (acetyl Lys16) antibody [1271] diluted at 1:500.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Immunohistochemistry-Paraffin-NBP2-42858-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of mouse kidney. Histone H4 (acetyl Lys16) antibody [1271] diluted at 1:500.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Immunohistochemistry-Paraffin-NBP2-42858-img0002.jpg)
![Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Rat brain. Histone H4K16ac (Acetyl Lys16) stained by Histone H4K16ac (Acetyl Lys16) antibody [1271] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-NBP2-42858-img0006.jpg)
![Western Blot: Histone H4 [ac Lys8] Antibody (GT478) [NBP3-13539] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 (acetyl Lys8) antibody (NBP3-13539) diluted at 1:1000.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-GT478-Western-Blot-NBP3-13539-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H4 [ac Lys8] Antibody (GT478) [NBP3-13539] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 (acetyl Lys8) antibody (NBP3-13539) diluted at 1:1000.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys8] Antibody (GT478) [NBP3-13539] - Histone H4 (acetyl Lys8) antibody [GT478] detects Histone H4 (acetyl Lys8) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (acetyl Lys8) protein stained by Histone H4 (acetyl Lys8) antibody [GT478] (NBP3-13539) diluted at 1:400. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (NBP2-20759) diluted at 1:300. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-GT478-Immunocytochemistry-Immunofluorescence-NBP3-13539-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody (GT478) [NBP3-13539] - Histone H4 (acetyl Lys8) antibody [GT478] detects Histone H4 (acetyl Lys8) protein at cytoplasm and nucleus in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Histone H4 (acetyl Lys8) antibody [GT478] (NBP3-13539) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-GT478-Immunohistochemistry-Paraffin-NBP3-13539-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody (GT478) [NBP3-13539] - Histone H4 (acetyl Lys8) antibody [GT478] detects Histone H4 (acetyl Lys8) protein at nucleus in human ovary by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. Histone H4 (acetyl Lys8) antibody [GT478] (NBP3-13539) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-GT478-Immunohistochemistry-Paraffin-NBP3-13539-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody (GT478) [NBP3-13539] - Histone H4 (acetyl Lys8) antibody [GT478] detects Histone H4 (acetyl Lys8) protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Histone H4 (acetyl Lys8) antibody [GT478] (NBP3-13539) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-GT478-Immunohistochemistry-Paraffin-NBP3-13539-img0004.jpg)
![Western Blot: Histone H4 [ac Lys8] Antibody [NBP2-59260] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody directed against H4K8ac diluted 1:200 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Western-Blot-NBP2-59260-img0001.jpg "Western Blot: Histone H4 [ac Lys8] Antibody [NBP2-59260] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody directed against H4K8ac diluted 1:200 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys8] Antibody [NBP2-59260] - Mouse NIH3T3 cells were stained with the antibody against H4K8ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H4K8ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunofluorescence-NBP2-59260-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NBP2-59260] - ChIP assays were performed using human HeLa cells, the antibody directed against H4K8ac and optimized PCR primer sets for qPCR. ChIP was performed with a LowCell l# ChIP kit on sheared chromatin from 10,000 cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the c-fos promoter and for the Sat2 satellite repeat region. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Chromatin-Immunoprecipitation-NBP2-59260-img0003.jpg)
![ELISA: Histone H4 [ac Lys8] Antibody [NBP2-59260] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H4K8ac, crude serum and flow through in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:16,700.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-ELISA-NBP2-59260-img0002.jpg)
![Dot Blot: Histone H4 [ac Lys8] Antibody [NBP2-59260] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H4K8ac with peptides containing other histone modifications and the unmodified H4K8. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Dot-Blot-NBP2-59260-img0006.jpg)
![Western Blot: Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody [NBP2-59252] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K5,8,12ac . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5--ac-Lys8--ac-Lys12]-Antibody-Western-Blot-NBP2-59252-img0001.jpg "Western Blot: Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody [NBP2-59252] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K5,8,12ac . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody [NBP2-59252] - HeLa cells were stained with Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody and DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. (Top): cells were immunofluorescently labeled with the Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. Subsequent images show staining of the cells with the Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody after incubation of the antibody with 10 ng/ul of the following blocking peptides: H4K5,8,12 unmodified, H4K5,8,12ac, H2A.ZK5,7,11ac and H4K5,8,12,16ac, respectively.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5--ac-Lys8--ac-Lys12]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59252-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody [NBP2-59252] - ChIP assays were performed using human K562 cells, the antibody against H4K5,8,12ac and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP- seq kit on sheared chromatin from 100,000 cells. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH gene, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5-ac-Lys8-ac-Lys12]-Antibody-Chromatin-Immunoprecipitation-NBP2-59252-img0003.jpg)
![ELISA: Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody [NBP2-59252] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H4K5,8,12ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:14,500.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5--ac-Lys8--ac-Lys12]-Antibody-ELISA-NBP2-59252-img0002.jpg)
![Dot Blot: Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody [NBP2-59252] - To test the cross reactivity of Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5--ac-Lys8--ac-Lys12]-Antibody-Dot-Blot-NBP2-59252-img0004.jpg)
![Western Blot: Histone H4 [ac Lys12] Antibody [NBP2-59266] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K12ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys12]-Antibody-Western-Blot-NBP2-59266-img0001.jpg "Western Blot: Histone H4 [ac Lys12] Antibody [NBP2-59266] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K12ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys12] Antibody [NBP2-59266] - HeLa cells were stained with the antibody against H4K12ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4K12ac antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys12]-Antibody-Immunofluorescence-NBP2-59266-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys12] Antibody [NBP2-59266] - ChIP assays were performed using human HeLa cells, the antibody against H4K12ac and optimized PCR primer sets for qPCR. ChIP was performed with the ChIP-seq kit on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active GAPDH and EIF4A2 genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys12]-Antibody-Chromatin-Immunoprecipitation-NBP2-59266-img0003.jpg)
![ELISA: Histone H4 [ac Lys12] Antibody [NBP2-59266] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H4K12ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:14,300.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys12]-Antibody-ELISA-NBP2-59266-img0002.jpg)
![Dot Blot: Histone H4 [ac Lys12] Antibody [NBP2-59266] - To test the cross reactivity of the antibody against H4K12ac, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:1,000. Figure shows the antibody is specific for the K12 acetylation with some slight cross reaction with K8ac.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys12]-Antibody-Dot-Blot-NBP2-59266-img0007.jpg)
![Western Blot: Histone H4 [ac Lys5] Antibody [NBP2-59254] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K5ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5]-Antibody-Western-Blot-NBP2-59254-img0001.jpg "Western Blot: Histone H4 [ac Lys5] Antibody [NBP2-59254] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K5ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The marker (kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys5] Antibody [NBP2-59254] - HeLa cells were stained with the antibody against H4K5ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4K5ac antibody (left) diluted 1:500 in blocking solution followed by an antirabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5]-Antibody-Immunofluorescence-NBP2-59254-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys5] Antibody [NBP2-59254] - ChIP assays were performed using human HeLa cells, the antibody against H4K5ac and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit on sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH gene, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5]-Antibody-Chromatin-Immunoprecipitation-NBP2-59254-img0003.jpg)
![ELISA: Histone H4 [ac Lys5] Antibody [NBP2-59254] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H4K5ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:8,900.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5]-Antibody-ELISA-NBP2-59254-img0002.jpg)
![Dot Blot: Histone H4 [ac Lys5] Antibody [NBP2-59254] - One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:5,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5]-Antibody-Dot-Blot-NBP2-59254-img0005.jpg)
![Western Blot: Histone H4 Antibody [NB100-56348] - Analysis of Histone H4 in human PBMC cell lysate with NB100-56348.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Western-Blot-NB100-56348-img0004.jpg "Western Blot: Histone H4 Antibody [NB100-56348] - Analysis of Histone H4 in human PBMC cell lysate with NB100-56348.")
![Histone H4 [ac Lys12] Antibody (RM202) - Azide and BSA Free [NBP3-26016] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate](http://images.novusbio.com/fullsize/nbp3-26016_rabbit-histone-h4-mab-rm202-azide-and-bsa-free-20620241532491.png "Histone H4 [ac Lys12] Antibody (RM202) - Azide and BSA Free [NBP3-26016] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate")
![Multiplex Immunoassay: Histone H4 [ac Lys12] Antibody (RM202) - Azide and BSA Free [NBP3-26016] - RM202 specifically reacts to Histone H4 acetylated at Lysine 12 (K12ac). No cross reactivity with unmodified Lysine 12 (K126 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 16 (K16ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.](http://images.novusbio.com/fullsize/nbp3-26016_rabbit-histone-h4-mab-rm202-azide-and-bsa-free-2462024133249.png)
![Western Blot: Histone H4 [ac Lys12] Antibody (RM202) - Azide and BSA Free [NBP3-26016] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated, using RM202 at 0.5 ug/mL, showed a band of Histone H4 acetylated at Lysine 12 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-26016_rabbit-histone-h4-mab-rm202-azide-and-bsa-free-22620242311076.png)
![Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys12] Antibody (RM202) - Azide and BSA Free [NBP3-26016] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM202 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-26016_rabbit-histone-h4-mab-rm202-azide-and-bsa-free-22620242311034.png)