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![Western Blot: OSBPL11 Antibody (6A6) [NBP2-03327] Analysis of extracts (35ug) from 9 different cell lines by using anti-OSBPL11 monoclonal antibody.](http://images.novusbio.com/fullsize/OSBPL11-Antibody-6A6-Western-Blot-NBP2-03327-img0005.jpg "Western Blot: OSBPL11 Antibody (6A6) [NBP2-03327] Analysis of extracts (35ug) from 9 different cell lines by using anti-OSBPL11 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: OSBPL11 Antibody (6A6) [NBP2-03327] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY OSBPL11.](http://images.novusbio.com/fullsize/OSBPL11-Antibody-6A6-Immunocytochemistry-Immunofluorescence-NBP2-03327-img0004.jpg)
![Flow Cytometry: OSBPL11 Antibody (6A6) [NBP2-03327] - Analysis of Jurkat cells, using anti-OSBPL11 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/OSBPL11-Antibody-6A6-Flow-Cytometry-NBP2-03327-img0002.jpg)
![Western Blot: OSBPL11 Antibody (6A6) [NBP2-03327] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBPL11 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBPL11.](http://images.novusbio.com/fullsize/OSBPL11-Antibody-6A6-Western-Blot-NBP2-03327-img0003.jpg)
![Flow Cytometry: OSBPL11 Antibody (6A6) [NBP2-03327] - Analysis of Hela cells, using anti-OSBPL11 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/OSBPL11-Antibody-6A6-Flow-Cytometry-NBP2-03327-img0001.jpg)
![Western Blot: OSBPL11 Antibody [NB100-1337] - (1ug/ml) of Human Brain lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/OSBPL11-Antibody-Western-Blot-NB100-1337-img0003.jpg "Western Blot: OSBPL11 Antibody [NB100-1337] - (1ug/ml) of Human Brain lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Western Blot: OSBPL11 Antibody - BSA Free [NBP3-47251] - Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with (OSBPL11 antibody) at dilution of 1:500](http://images.novusbio.com/fullsize/nbp3-47251_rabbit-osbpl11-pab-bsa-free-28420251235653.jpg "Western Blot: OSBPL11 Antibody - BSA Free [NBP3-47251] - Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with (OSBPL11 antibody) at dilution of 1:500")
![Immunohistochemistry: OSBPL11 Antibody - BSA Free [NBP3-47251] - Immunohistochemistry of paraffin-embedded human ovary tumor using (OSBPL11 antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-47251_rabbit-osbpl11-pab-bsa-free-284202512283367.jpg)
![Immunoprecipitation: OSBPL11 Antibody - BSA Free [NBP3-47251] - IP Result of anti-OSBPL11 (IP:, 4ug; Detection: 1:300) with mouse testis tissue lysate 4000ug.](http://images.novusbio.com/fullsize/nbp3-47251_rabbit-osbpl11-pab-bsa-free-284202512315780.jpg)
![Western Blot: OSBPL11 Antibody [NBP2-36544] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ORP11 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/OSBPL11-Antibody-Western-Blot-NBP2-36544-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: OSBPL11 Antibody [NBP2-36544] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ORP11 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: OSBPL11 Antibody [NBP2-36544] - Detection of human ORP11 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ORP11 antibody NBP2-36544 used for IP at 6 ug per reaction. ORP11 was also immunoprecipitated by rabbit anti-ORP11 antibody NBP2-36545. For blotting immunoprecipitated ORP11, NBP2-36544 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/OSBPL11-Antibody-Immunoprecipitation-NBP2-36544-img0002.jpg)
![Western Blot: OSBPL11 Antibody [NBP2-36545] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antiORP11 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/OSBPL11-Antibody-Western-Blot-NBP2-36545-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: OSBPL11 Antibody [NBP2-36545] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antiORP11 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: OSBPL11 Antibody [NBP2-36545] - Detection of human ORP11 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ORP11 antibody NBP2-36545 used for IP at 6 ug per reaction. ORP11 was also immunoprecipitated by rabbit anti-ORP11 antibody NBP2-36544. For blotting immunoprecipitated ORP11, NBP2-36545 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/OSBPL11-Antibody-Immunoprecipitation-NBP2-36545-img0002.jpg)
![Western Blot: OSBPL11 Antibody [NBP3-10059] - Western blot analysis of OSBPL11 in Human Liver Tumor lysates. Antibody dilution at 1ug/ml](http://images.novusbio.com/fullsize/OSBPL11-Antibody-Western-Blot-NBP3-10059-img0001.jpg "Western Blot: OSBPL11 Antibody [NBP3-10059] - Western blot analysis of OSBPL11 in Human Liver Tumor lysates. Antibody dilution at 1ug/ml")
![Western Blot: OSBPL11 Overexpression Lysate (Adult Normal) [NBL1-13986] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OSBPL11.](http://images.novusbio.com/fullsize/OSBPL11-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13986-img0002.jpg "Western Blot: OSBPL11 Overexpression Lysate (Adult Normal) [NBL1-13986] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OSBPL11.")