| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86881-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74805 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38203-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74805 | |||
![Immunocytochemistry/Immunofluorescence: USP16 Antibody [NBP1-86881] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/USP16-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86881-img0007.jpg "Immunocytochemistry/Immunofluorescence: USP16 Antibody [NBP1-86881] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.")
![Immunohistochemistry-Paraffin: USP16 Antibody [NBP1-86881] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/USP16-Antibody-Immunohistochemistry-Paraffin-NBP1-86881-img0011.jpg)
![Immunohistochemistry-Paraffin: USP16 Antibody [NBP1-86881] - Staining of human gastrointestinal shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/USP16-Antibody-Immunohistochemistry-Paraffin-NBP1-86881-img0008.jpg)
![Immunohistochemistry-Paraffin: USP16 Antibody [NBP1-86881] - Staining of human kidney shows strong nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/USP16-Antibody-Immunohistochemistry-Paraffin-NBP1-86881-img0009.jpg)
![Immunohistochemistry-Paraffin: USP16 Antibody [NBP1-86881] - Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/USP16-Antibody-Immunohistochemistry-Paraffin-NBP1-86881-img0010.jpg)
![Western Blot: USP16 Antibody (1B4) [NBP2-46305] - E.coli lysate (5 ug, left lane) and E.coli lysate expressing human recombinant protein fragment (5 ug, right lane) corresponding to amino acids 137-466 of human USP16(NP_001001992) were separated by SDS-PAGE and immunoblotted with USP16.](http://images.novusbio.com/fullsize/USP16-Antibody-1B4-Western-Blot-NBP2-46305-img0001.jpg "Western Blot: USP16 Antibody (1B4) [NBP2-46305] - E.coli lysate (5 ug, left lane) and E.coli lysate expressing human recombinant protein fragment (5 ug, right lane) corresponding to amino acids 137-466 of human USP16(NP_001001992) were separated by SDS-PAGE and immunoblotted with USP16.")
![Western Blot: USP16 Antibody (1B4) [NBP2-46305] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/USP16-Antibody-1B4-Western-Blot-NBP2-46305-img0002.jpg)
![Western Blot: USP16 Antibody [H00010600-B01P] - Analysis of USP16 expression in transfected 293T cell line by USP16 polyclonal antibody. Lane 1: USP16 transfected lysate(90.42 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/USP16-Antibody-Western-Blot-H00010600-B01P-img0004.jpg "Western Blot: USP16 Antibody [H00010600-B01P] - Analysis of USP16 expression in transfected 293T cell line by USP16 polyclonal antibody. Lane 1: USP16 transfected lysate(90.42 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: USP16 Antibody [H00010600-B01P] - Analysis of purified antibody to USP16 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/USP16-Antibody-Immunocytochemistry-Immunofluorescence-H00010600-B01P-img0003.jpg)
![Western Blot: USP16 Antibody [NB100-81638] - Detection of Human USP16 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP16 antibody NB100-81638 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/USP16-Antibody-Western-Blot-NB100-81638-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: USP16 Antibody [NB100-81638] - Detection of Human USP16 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP16 antibody NB100-81638 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: USP16 Antibody [NB100-81638] - Detection of Human USP16 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP16 antibody NB100-81638 used for IP at 3 mcg/mg lysate. USP16 was also immunoprecipitated by rabbit anti- USP16 antibody NB100-81639, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/USP16-Antibody-Western-Blot-NB100-81638-img0001.jpg)
![Western Blot: USP16 Antibody [NBP3-38203] - Western blot analysis of various lysates, using USP16 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38203_rabbit-usp16-pab-912202415111853.jpg "Western Blot: USP16 Antibody [NBP3-38203] - Western blot analysis of various lysates, using USP16 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunoprecipitation: USP16 Antibody [NB100-81639] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cellsprepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP16 antibody used for IP at 6 ug per reaction. USP16 was also immunoprecipitated by rabbit anti-USP16 antibody. For blotting immunoprecipitated USP16 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/USP16-Antibody-Immunoprecipitation-NB100-81639-img0001.jpg "Immunoprecipitation: USP16 Antibody [NB100-81639] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cellsprepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP16 antibody used for IP at 6 ug per reaction. USP16 was also immunoprecipitated by rabbit anti-USP16 antibody. For blotting immunoprecipitated USP16 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: USP16 Overexpression Lysate (Adult Normal) [NBL1-17647] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USP16.](http://images.novusbio.com/fullsize/USP16-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17647-img0002.jpg "Western Blot: USP16 Overexpression Lysate (Adult Normal) [NBL1-17647] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USP16.")
![Western Blot: USP16 Overexpression Lysate (Adult Normal) [NBP2-09188] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USP16.](http://images.novusbio.com/fullsize/USP16-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09188-img0001.jpg "Western Blot: USP16 Overexpression Lysate (Adult Normal) [NBP2-09188] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USP16.")
![Western Blot: USP16 Overexpression Lysate (Adult Normal) [NBP2-09831] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USP16.](http://images.novusbio.com/fullsize/USP16-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09831-img0001.jpg "Western Blot: USP16 Overexpression Lysate (Adult Normal) [NBP2-09831] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USP16.")