MAB32653) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32654) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human Mesothelin protein was serially diluted 2-fold and captured by Mouse Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32653) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32654) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB32654-SP | |||
NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Mesothelin was detected in immersion fixed Capan‑2 human pancreatic adenocarcinoma cells (positive), and absent in K562 human chronic myelogenous leukemia cells (negative) using Mouse Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB11417) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB11417-SP | |||
DY990). Rat Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32652) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human Mesothelin protein was serially diluted 2-fold and captured by Mouse Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32655) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32652) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB32655-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20893 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21887-50ul | |||
![Western Blot: Mesothelin Antibody (5R9H8) [NBP3-16777] - Analysis of extracts of various cell lines, using Mesothelin antibody (NBP3-16777) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Mesothelin-Antibody-5R9H8-Western-Blot-NBP3-16777-img0002.jpg "Western Blot: Mesothelin Antibody (5R9H8) [NBP3-16777] - Analysis of extracts of various cell lines, using Mesothelin antibody (NBP3-16777) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: Mesothelin Antibody (5R9H8) [NBP3-16777] - Confocal imaging of paraffin-embedded Rat lung using Mesothelin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16777_rabbit-mesothelin-mab-5r9h8-522025125481.jpg)
![Immunohistochemistry: Mesothelin Antibody (PSH0-57) [NBP3-32592] - Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue with Rabbit anti-Mesothelin antibody (NBP3-32592) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32592) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32592_rabbit-mesothelin-mab-psh0-57-298202414262646.jpg "Immunohistochemistry: Mesothelin Antibody (PSH0-57) [NBP3-32592] - Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue with Rabbit anti-Mesothelin antibody (NBP3-32592) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32592) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: Mesothelin Antibody (PSH0-57) [NBP3-32592] - Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling Mesothelin with Rabbit anti-Mesothelin antibody (NBP3-32592) at 1/100 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32592, green) at 1/100 dilution overnight at 4 ℃, washed with PBS.<br/><br/> Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32592_rabbit-mesothelin-mab-psh0-57-298202414304152.jpg)
![Immunohistochemistry: Mesothelin Antibody (PD00-68) [NBP3-32591] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Mesothelin antibody (NBP3-32591) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32591) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32591_rabbit-mesothelin-mab-pd00-68-29820241416180.jpg "Immunohistochemistry: Mesothelin Antibody (PD00-68) [NBP3-32591] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Mesothelin antibody (NBP3-32591) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32591) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32591 at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: SK-OV-3 cell lysate Lane 3: OVCAR-3 cell lysate Lane 4: SiHa cell lysate Lane 5: NCI-H226 cell lysate Lane 6: PC-3M cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 69 kDa Observed band size: 45 kDa Exposure time: 7 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32591 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32591_-western-blot-639204594550605557.jpg)
![Western Blot: Mesothelin Overexpression Lysate (Adult Normal) [NBL1-13332] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Mesothelin.](http://images.novusbio.com/fullsize/Mesothelin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13332-img0002.jpg "Western Blot: Mesothelin Overexpression Lysate (Adult Normal) [NBL1-13332] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Mesothelin.")
![Western Blot: Mesothelin Antibody (SR2132) [NBP3-21887] - Western blot analysis using NBP3-21887 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21887_rabbit-mesothelin-mab-sr2132-5720231754869.jpg "Western Blot: Mesothelin Antibody (SR2132) [NBP3-21887] - Western blot analysis using NBP3-21887 in HeLa cell lysate.")
