HAF019). A specific band was detected for Mesothelin at approximately 69 kDa (as indicated). This experiment was conducted under reducing conditions and Immunoblot Buffer Group 8." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Mesothelin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3265) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Mesothelin at approximately 69 kDa (as indicated). This experiment was conducted under reducing conditions and Immunoblot Buffer Group 8." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3265-SP | |||
5609-MU) is coated at 0.2 μg/mL (100 μL/well), Biotinylated Recombinant Human Mesothelin C-terminal (aa 296‑580) Fc Chimera Avi‑tag (Catalog # AVI10679) binds with an ED50 of 2-12 ng/mL." alt="When Recombinant Human CA125/MUC16 (5609-MU) is coated at 0.2 μg/mL (100 μL/well), Biotinylated Recombinant Human Mesothelin C-terminal (aa 296‑580) Fc Chimera Avi‑tag (Catalog # AVI10679) binds with an ED50 of 2-12 ng/mL." class="big_thumb" />
5609-MU) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Cynomolgus Mesothelin (aa 417-693) Fc Chimera (Catalog # 10760-MS) binds with an ED50 of 4.0-20.0 ng/mL." alt="When Recombinant Human CA125/MUC16 (5609-MU) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Cynomolgus Mesothelin (aa 417-693) Fc Chimera (Catalog # 10760-MS) binds with an ED50 of 4.0-20.0 ng/mL." class="big_thumb" />
5609-MU) is immobilized at 2.00 μg/mL (100 μg/well), Recombinant Cynomolgus Monkey Mesothelin C-Terminal (aa 417-693) His-tag (Catalog # 10764-MS) binds with an ED50 of 0.700-4.20 μg/mL." alt="When Recombinant Human CA125/MUC16 (5609-MU) is immobilized at 2.00 μg/mL (100 μg/well), Recombinant Cynomolgus Monkey Mesothelin C-Terminal (aa 417-693) His-tag (Catalog # 10764-MS) binds with an ED50 of 0.700-4.20 μg/mL." class="big_thumb" />
5609-MU) is immobilized at 200 ng/mL (100 µL/well), Recombinant Human Mesothelin (aa 296-580) Fc Chimera Protein (Catalog # 10959-MS) binds with an ED50 of 1.00-6.00 ng/mL." alt="When Recombinant Human CA125/MUC16 (5609-MU) is immobilized at 200 ng/mL (100 µL/well), Recombinant Human Mesothelin (aa 296-580) Fc Chimera Protein (Catalog # 10959-MS) binds with an ED50 of 1.00-6.00 ng/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3265-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3448-SP | |||
![Immunohistochemistry: Mesothelin Antibody (PSH02-30) [NBP3-32593] - Immunofluorescence analysis of paraffin-embedded human ovarian carcinoma tissue labeling Mesothelin with Rabbit anti-Mesothelin antibody (NBP3-32593) at 1/100 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32593, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32593_rabbit-mesothelin-mab-psh02-30-29820241492656.jpg "Immunohistochemistry: Mesothelin Antibody (PSH02-30) [NBP3-32593] - Immunofluorescence analysis of paraffin-embedded human ovarian carcinoma tissue labeling Mesothelin with Rabbit anti-Mesothelin antibody (NBP3-32593) at 1/100 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32593, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Mesothelin Antibody (PSH02-30) [NBP3-32593] - Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue with Rabbit anti-Mesothelin antibody (NBP3-32593) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32593) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32593_rabbit-mesothelin-mab-psh02-30-298202414161861.jpg)
![Immunocytochemistry/ Immunofluorescence: Mesothelin Antibody (PSH02-30) [NBP3-32593] - Immunocytochemistry analysis of NCI-H226 cells labeling Mesothelin with Rabbit anti-Mesothelin antibody (NBP3-32593) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Mesothelin antibody (NBP3-32593) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32593_rabbit-mesothelin-mab-psh02-30-2920241192384.jpg)
![Western blot analysis on different lysates with NBP3-32593 at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: NCI-H226 cell lysate Lane 3: HeLa cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lane 5: PC-3M cell lysate (negative) Lane 6: A549 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 68 kDa Observed band size: 40 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32593 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32593_-western-blot-639204597139334141.jpg)
![N/A Mesothelin [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Mesothelin_DY3448_ELISA_1942.jpg "N/A Mesothelin [Biotin]")
