Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: Mesothelin Antibody (PSH02-30) [NBP3-32593] - Western blot analysis of Mesothelin on different lysates with Rabbit anti-Mesothelin antibody (NBP3-32593) at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: NCI-H226 cell lysate Lane 3: HeLa cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lane 5: PC-3M cell lysate (negative) Lane 6: A549 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 68 kDa Observed band size: 40 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32593) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32593_rabbit-mesothelin-mab-psh02-30-27820241529554.jpg "Western Blot: Mesothelin Antibody (PSH02-30) [NBP3-32593] - Western blot analysis of Mesothelin on different lysates with Rabbit anti-Mesothelin antibody (NBP3-32593) at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: NCI-H226 cell lysate Lane 3: HeLa cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lane 5: PC-3M cell lysate (negative) Lane 6: A549 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 68 kDa Observed band size: 40 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32593) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Mesothelin Antibody (PSH02-30) [NBP3-32593] - Immunofluorescence analysis of paraffin-embedded human ovarian carcinoma tissue labeling Mesothelin with Rabbit anti-Mesothelin antibody (NBP3-32593) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32593, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32593_rabbit-mesothelin-mab-psh02-30-29820241492656.jpg)
![Immunohistochemistry: Mesothelin Antibody (PSH02-30) [NBP3-32593] - Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue with Rabbit anti-Mesothelin antibody (NBP3-32593) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32593) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32593_rabbit-mesothelin-mab-psh02-30-298202414161861.jpg)
![Immunocytochemistry/ Immunofluorescence: Mesothelin Antibody (PSH02-30) [NBP3-32593] - Immunocytochemistry analysis of NCI-H226 cells labeling Mesothelin with Rabbit anti-Mesothelin antibody (NBP3-32593) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Mesothelin antibody (NBP3-32593) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32593_rabbit-mesothelin-mab-psh02-30-2920241192384.jpg)
![N/A Mesothelin [Biotin]](http://images.novusbio.com/fullsize2/DATA_Mesothelin_DY3448_ELISA_1942.jpg "N/A Mesothelin [Biotin]")
