| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57361-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49481-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55886-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38552-20ul | |||
![Flow Cytometry: PATJ Antibody [NB100-1354] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PATJ-Antibody-Flow-Cytometry-NB100-1354-img0004.jpg "Flow Cytometry: PATJ Antibody [NB100-1354] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Immunofluorescence: PATJ Antibody [NB100-1354] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing junctional staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/PATJ-Antibody-Immunofluorescence-NB100-1354-img0003.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PATJ antibody [HL3842] (NBP3-48849) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48849_rabbit-patj-mab-hl3842-western-blot-141120251839540.jpg "<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PATJ antibody [HL3842] (NBP3-48849) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>PATJ antibody [HL3842] detects PATJ protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.PATJ stained by PATJ antibody [HL3842] (NBP3-48849) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48849_rabbit-patj-mab-hl3842-immunohistochemistry-paraffin-1411202518442217.jpg)
![Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PATJ antibody [HL3842] (NBP3-48849) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48849_rabbit-patj-mab-hl3842-western-blot-1411202518414121.jpg)
![Immunocytochemistry/Immunofluorescence: PATJ Antibody [NBP2-57361] - Staining of human cell line A-431 shows localization to microtubule organizing center & cell junctions.](http://images.novusbio.com/fullsize/PATJ-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57361-img0001.jpg "Immunocytochemistry/Immunofluorescence: PATJ Antibody [NBP2-57361] - Staining of human cell line A-431 shows localization to microtubule organizing center & cell junctions.")
![Immunohistochemistry: PATJ Antibody [NBP2-49481] - Staining of human colon shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/PATJ-Antibody-Immunohistochemistry-NBP2-49481-img0001.jpg "Immunohistochemistry: PATJ Antibody [NBP2-49481] - Staining of human colon shows strong membranous positivity in glandular cells.")
![Immunocytochemistry/Immunofluorescence: PATJ Antibody [NBP2-55886] - Staining of human cell line A-431 shows localization to cytosol, microtubule organizing center & cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PATJ-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55886-img0001.jpg "Immunocytochemistry/Immunofluorescence: PATJ Antibody [NBP2-55886] - Staining of human cell line A-431 shows localization to cytosol, microtubule organizing center & cell junctions. Antibody staining is shown in green.")
![Western Blot: PATJ Antibody [NBP3-38552] - Western blot analysis of various lysates using PATJ Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-38552_rabbit-patj-pab-91220241418199.jpg "Western Blot: PATJ Antibody [NBP3-38552] - Western blot analysis of various lysates using PATJ Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Western Blot: PATJ Antibody [NBP1-58843] - 293T cells lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/PATJ-Antibody-Western-Blot-NBP1-58843-img0002.jpg "Western Blot: PATJ Antibody [NBP1-58843] - 293T cells lysate, concentration 0.2-1 ug/ml.")