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VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " class="big_lightbox" target="_blank">
622-VD) was serially diluted and captured by Mouse Anti-Human VEGF‑D Monoclonal Antibody (Catalog # MAB2861) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human VEGF‑D Monoclonal Antibody (Catalog # MAB286) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in pneumocytes in lung. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
469-VD) was serially diluted and captured by Rat Anti-Mouse VEGF‑D Monoclonal Antibody (Catalog # MAB469) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse VEGF‑D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF469) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
622-VD) stimulates proliferation in HMVEC human microvascular endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human VEGF-D (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human VEGF-D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF286). The ND50 is typically 3-10 µg/mL." class="big_lightbox" target="_blank">
Vascular Endothelial Growth Factor D (VEGFD), also known as c-fos-induced growth factor (FIGF), is a member of the VEGF family of growth factors. Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. VEGFD is most closely related to VEGFC (23.3% amino acid sequence identity) and has a similar VEGF homology domain that spans the middle third of the precursor protein and the long N-terminal and C-terminal extensions. In adults, VEGFD is highly expressed in lung, heart, muscle, and small intestine. VEGFD expression in fibroblasts is induced by cell interaction mediated by cadherin 11. Recombinant human VEGFD is a ligand for the tyrosine kinases, VEGFR2 (Flk1) and VEGF receptor 3 (Flt4). Since VEGFR3 is strongly expressed in lymphatic endothelial cells, it is postulated that VEGFD is involved in the regulation of the growth and/or differentiation of lymphatic endothelium and thus, a mitogen for endothelial cells.
![Immunohistochemistry VEGF-D Antibody (78923) [Unconjugated]](http://images.novusbio.com/images/antibody/mab286_human-vegf-d-mab-clone-78923-immunohistochemistry-182023124954.jpg)
![Immunohistochemistry VEGF-D Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/VEGFD_AF469_Immunohistochemistry_19559.jpg)
![Neutralization VEGF-D Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/VEGF-D_AF286_Block_Neutralize_8841.jpg)
![N/A VEGF-D [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_VEGFD_DVED00_ELISA_875.jpg)
![N/A VEGF-D [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_VEGFD_DY622_ELISA_2154.jpg)
![ELISA VEGF-D Antibody (78923) [Unconjugated]](http://images.novusbio.com/images/mab286_human-vegf-d-mab-clone-78923-elisa-639201856339938797.jpg)
![Immunohistochemistry VEGF-D Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af469_mouse-vegf-d-affinity-purified-polyclonal-ab-immunohistochemistry-121220259214815.jpg)
![Immunohistochemistry VEGF-D Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af3690_human-mouse-gli-3-affinity-purified-polyclonal-ab-immunocytochemistry-immunofluorescence-121220258481620.jpg)
![ELISA VEGF-D Antibody [Unconjugated]](http://images.novusbio.com/images/af469_mouse-vegf-d-affinity-purified-polyclonal-ab-elisa-639201856339808471.jpg)
![N/A VEGF-D [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_VEGFD_DVED00_ELISA_876.jpg)
![N/A VEGF-D [HRP]](http://images.novusbio.com/fullsize/elisa/VEGF-D_DVED00_ELISA_210.jpg)
![Knockdown Validated VEGF-D [HRP]](http://images.novusbio.com/fullsize/dved00_human-vegf-d-quantikine-elisa-kit-4420241542507.jpg)
