NCAM-1/CD56 Research Products: Novus Biologicals

Antibodies

NCAM-1/CD56 Antibody [Unconju ...

AF2408

HAF017). Specific bands were detected for NCAM-1/CD56 at approximately 100-150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank"> Western Blot NCAM-1/CD56 Antibody [Unconjugated]

Western Blot NCAM-1/CD56 Antibody [Unconjugated]

NL001). Actin filaments were stained with FITC-conjugated Phalloidin (green) and cell nuclei were counter-stained with DAPI (blue). NCAM-1/CD56 immuno-reactivity was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox" target="_blank"> Immunocytochemistry NCAM-1/CD56 Antibody [Unconjugated]

Immunocytochemistry NCAM-1/CD56 Antibody [Unconjugated]

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." class="big_lightbox" target="_blank"> Immunocytochemistry NCAM-1/CD56 Antibody [Unconjugated]

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox" target="_blank"> Simple Western NCAM-1/CD56 Antibody [Unconjugated]

Simple Western NCAM-1/CD56 Antibody [Unconjugated]

HAF017). A specific band was detected for NCAM‑1/CD56 at approximately 160 kDa (as indicated) in the parental U937 human histiocytic lymphoma cell line, but is not detectable in knockout U937 human histiocytic lymphoma cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox" target="_blank"> Knockout Validated NCAM-1/CD56 Antibody [Unconjugated]

Knockout Validated NCAM-1/CD56 Antibody [Unconjugated]

2408-NC) was serially diluted and captured by Mouse Anti-Human/Primate NCAM‑1/CD56 Monoclonal Antibody (Catalog # MAB2408) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse NCAM‑1/CD56 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2408) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank"> ELISA NCAM-1/CD56 Antibody [Unconjugated]

ELISA NCAM-1/CD56 Antibody [Unconjugated]

(27 Publications)

Species: Hu, Mu
Applications: WB, Simple Western, Flow, CyTOF-ready, ICC/IF, KO
Host: Goat Polyclonal

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2408U-100UG
Alexa Fluor 405	AF2408V-100UG
Alexa Fluor 488	AF2408G-100UG
Alexa Fluor 532	AF2408X-100UG
Alexa Fluor 594	AF2408T-100UG
Alexa Fluor 647	AF2408R-100UG
Alexa Fluor 700	AF2408N-100UG
Alexa Fluor 750	AF2408S-100UG
Alexa Fluor Plus 405	AF2408AFP405-100UG
Alexa Fluor Plus 488	AF2408AFP488-100UG
Alexa Fluor Plus 555	AF2408AFP555-100UG
Alexa Fluor Plus 594	AF2408AFP594-100UG
Alexa Fluor Plus 647	AF2408AFP647-100UG
Alexa Fluor Plus 680	AF2408AFP680-100UG
Allophycocyanin	AF2408A
Biotin	BAF2408
CoraFluor 1	AF2408CL1
DyLight 350	AF2408D
DyLight 405	AF2408E
DyLight 488	AF2408K
DyLight 550	AF2408L
DyLight 594	AF2408M
DyLight 650	AF2408W
DyLight 680	AF2408Y
DyLight 755	AF2408Z
FITC	AF2408F
HRP	AF2408H
Janelia Fluor 525	AF2408JF525
Janelia Fluor 549	AF2408I
Janelia Fluor 646	AF2408J
Janelia Fluor 669	AF2408JF669
PE	AF2408P
PerCP	AF2408C
mFluor Violet 450 SE	AF2408MFV450
mFluor Violet 500 SE	AF2408MFV500
mFluor Violet 610 SE	AF2408MFV610

Formulation	Catalog #	Availability	Price
Carrier Free	AF2408-SP

NCAM-1/CD56 Antibody (123C3.D ...

NBP2-15184

Flow Cytometry NCAM-1/CD56 Antibody (123C3.D5)

Simple Western NCAM-1/CD56 Antibody (123C3.D5)

Western Blot NCAM-1/CD56 Antibody (123C3.D5)

(2 Reviews)

(8 Publications)

Species: Hu, Rt
Applications: Simple Western, Flow, ICC/IF, IHC
Host: Mouse Monoclonal

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-33132AF350
Alexa Fluor 405	NBP2-33132AF405
Alexa Fluor 488	NBP2-33132AF488
Alexa Fluor 532	NBP2-33132AF532
Alexa Fluor 594	NBP2-33132AF594
Alexa Fluor 647	NBP2-33132AF647
Alexa Fluor 700	NBP2-33132AF700
Alexa Fluor 750	NBP2-33132AF750
Alexa Fluor Plus 405	NBP2-33132AFP405
Alexa Fluor Plus 488	NBP2-33132AFP488
Alexa Fluor Plus 555	NBP2-33132AFP555
Alexa Fluor Plus 594	NBP2-33132AFP594
Alexa Fluor Plus 647	NBP2-33132AFP647
Alexa Fluor Plus 680	NBP2-33132AFP680
Allophycocyanin	NBP2-33132APC
Allophycocyanin/Cy7	NBP2-33132APCCY7
Biotin	NBP2-33132B
CoraFluor 1	NBP2-33132CL1
DyLight 350	NBP2-33132UV
DyLight 405	NBP2-33132V
DyLight 488	NBP2-33132G
DyLight 550	NBP2-33132R
DyLight 594	NBP2-33132DL594
DyLight 650	NBP2-33132C
DyLight 680	NBP2-33132FR
DyLight 755	NBP2-33132IR
FITC	NBP2-33132F
HRP	NBP2-33132H
Janelia Fluor 525	NBP2-33132JF525
Janelia Fluor 549	NBP2-33132JF549
Janelia Fluor 646	NBP2-33132JF646
Janelia Fluor 669	NBP2-33132JF669
PE	NBP2-33132PE
PE/Atto594	NBP2-33132PEATT594
PE/Cy5.5	NBP2-33132PECY55
PE/Cy7	NBP2-33132PECY7
PerCP	NBP2-33132PCP
mFluor Violet 450 SE	NBP2-33132MFV450
mFluor Violet 500 SE	NBP2-33132MFV500
mFluor Violet 610 SE	NBP2-33132MFV610

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-33132
IHC-Prediluted	NBP2-44711

NCAM-1/CD56 Antibody (301021) ...

MAB24081

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the neuropil. Protocol available in COMET™ Panel Builder." class="big_lightbox" target="_blank"> Multiplex Immunofluorescence NCAM-1/CD56 Antibody (301021) [Unconjugated]

Multiplex Immunofluorescence NCAM-1/CD56 Antibody (301021) [Unconjugated]

HAF018). A specific band was detected for NCAM‑1/CD56 at approximately 105-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox" target="_blank"> Western Blot NCAM-1/CD56 Antibody (301021) [Unconjugated]

Western Blot NCAM-1/CD56 Antibody (301021) [Unconjugated]

MAB0041) followed by Anti-Mouse IgG APC-conjugated secondary antibody (F0101B) and Mouse Anti-Human NKp46 PE-conjugated Monoclonal Antibody (FAB1850P). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox" target="_blank"> Flow Cytometry NCAM-1/CD56 Antibody (301021) [Unconjugated]

Flow Cytometry NCAM-1/CD56 Antibody (301021) [Unconjugated]

CLD004) and were stained with either (A) Mouse Anti-Human NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB24081) or (B) Mouse IgG2B Isotype Control (MAB0041) followed by Anti-Mouse IgG APC-conjugated secondary antibody (F0101B) and Mouse Anti-HumanCD3 PE-conjugated Monoclonal Antibody (FAB100P). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox" target="_blank"> Flow Cytometry NCAM-1/CD56 Antibody (301021) [Unconjugated]

VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the neuropil. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank"> Immunohistochemistry NCAM-1/CD56 Antibody (301021) [Unconjugated]

Immunohistochemistry NCAM-1/CD56 Antibody (301021) [Unconjugated]

(12 Publications)

Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, mIF
Host: Mouse Monoclonal

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB24081U-100UG
Alexa Fluor 405	FAB24081V-100UG
Alexa Fluor 488	FAB24081G-100UG
Alexa Fluor 532	FAB24081X
Alexa Fluor 594	FAB24081T-100UG
Alexa Fluor 647	FAB24081R-100UG
Alexa Fluor 700	FAB24081N-100UG
Alexa Fluor 750	FAB24081S-100UG
Alexa Fluor Plus 405	FAB24081AFP405-100UG
Alexa Fluor Plus 488	FAB24081AFP488-100UG
Alexa Fluor Plus 555	FAB24081AFP555-100UG
Alexa Fluor Plus 594	FAB24081AFP594-100UG
Alexa Fluor Plus 647	FAB24081AFP647-100UG
Alexa Fluor Plus 680	FAB24081AFP680-100UG
Allophycocyanin	FAB24081A
Allophycocyanin/Cy7	FAB24081APCCY7
Biotin	FAB24081B
CoraFluor 1	FAB24081CL1
DyLight 350	FAB24081D
DyLight 405	FAB24081E
DyLight 488	FAB24081K
DyLight 550	FAB24081L
DyLight 594	FAB24081M
DyLight 650	FAB24081W
DyLight 680	FAB24081Y
DyLight 755	FAB24081Z
FITC	FAB24081F
HRP	FAB24081H
Janelia Fluor 525	FAB24081JF525
Janelia Fluor 549	FAB24081I
Janelia Fluor 646	FAB24081J
Janelia Fluor 669	FAB24081JF669
PE	FAB24081P
PE/Atto594	FAB24081PEATT594
PE/Cy5.5	FAB24081PECY55
PE/Cy7	FAB24081PECY7
PerCP	FAB24081C
mFluor Violet 450 SE	FAB24081MFV450
mFluor Violet 500 SE	FAB24081MFV500
mFluor Violet 610 SE	FAB24081MFV610

Formulation	Catalog #	Availability	Price
Carrier Free	MAB24081-SP
Carrier Free	FAB24081V-100UG
Carrier Free	FAB24081U-100UG
Carrier Free	FAB24081T-100UG
Carrier Free	FAB24081S-100UG
Carrier Free	FAB24081R-100UG
Carrier Free	FAB24081N-100UG
Carrier Free	FAB24081G-100UG

We have 78 NCAM-1/CD56 Antibodies

ELISA Kits

Human NCAM-1/CD56 DuoSet ELIS ...

DY2408

(6 Publications)

Species: Hu
Applications: ELISA

Mouse NCAM-1/CD56 ELISA Kit ( ...

NBP2-81180

Species: Mu
Applications: ELISA

Rat NCAM-1/CD56 ELISA Kit (Co ...

NBP2-81181

Species: Rt
Applications: ELISA

We have 5 NCAM-1/CD56 ELISA Kits

Lysates

NCAM-1/CD56 Overexpression Ly ...

NBL1-13501

Species: Hu
Applications: WB

We have 1 NCAM-1/CD56 Lysate

Proteins

Recombinant Human NCAM-1/CD56 ...

10136-NC

Binding Activity NCAM-1/CD56 [Unconjugated]

Species: Hu
Applications: Bioactivity

Formulation	Catalog #	Availability	Price
Carrier Free	10136-NC-050

Recombinant Mouse NCAM-1/CD56 ...

6070-NC

(1 Publication)

Species: Mu
Applications: Bioactivity

Formulation	Catalog #	Availability	Price
Carrier Free	6070-NC-050

Recombinant Human NCAM-1/CD56 ...

2408-NC

Species: Hu
Applications: Bioactivity

Formulation	Catalog #	Availability	Price
Carrier Free	2408-NC-050

We have 5 NCAM-1/CD56 Proteins

Description

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.

Bioinformatics

Uniprot	AAH47244 Human AAH47244.1 Human NM_181351 Human NP_851996.2 Human P13591 Human P13592 Human
Product By Gene ID	4684
Alternate Names	CD56 / NCAM-1 CD56 antigen CD56 MSK39 N-CAM-1 NCAM-1 NCAMantigen recognized by monoclonal 5.1H11 neural cell adhesion molecule 1 neural cell adhesion molecule, NCAM

Research Areas for NCAM-1/CD56

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Stem Cell Markers

NCAM-1/CD56 Products

Description

Bioinformatics

Research Areas for NCAM-1/CD56