Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51596-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37417-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52409-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61695-0.025ml | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-75127 | |||
| Biotin | NBP1-75128 | |||
| DyLight 350 | NBP1-75948 | |||
| DyLight 405 | NBP1-75949 | |||
| DyLight 488 | NBP1-75132 | |||
| DyLight 550 | NBP1-75950 | |||
| DyLight 594 | NBP1-75951 | |||
| DyLight 633 | NBP1-75952 | |||
| DyLight 650 | NBP1-75953 | |||
| FITC | NBP1-75129 | |||
| HRP | NBP1-75130 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05166SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05122-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23558-0.025ml | |||
![Western Blot: SMC1 Antibody (5B6) [NBP1-51596] - Analysis using SMC1 mouse mAb against K562 (1), Jurkat (2) and A549 (3) cell lysate.](http://images.novusbio.com/fullsize/SMC1-Antibody-5B6-Western-Blot-NBP1-51596-img0007.jpg "Western Blot: SMC1 Antibody (5B6) [NBP1-51596] - Analysis using SMC1 mouse mAb against K562 (1), Jurkat (2) and A549 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: SMC1 Antibody (5B6) [NBP1-51596] - Analysis of NIH3T3 cells using SMC1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/SMC1-Antibody-5B6-Immunocytochemistry-Immunofluorescence-NBP1-51596-img0010.jpg)
![Immunohistochemistry-Paraffin: SMC1 Antibody (5B6) [NBP1-51596] - Analysis of paraffin-embedded human colon using SMC1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/SMC1-Antibody-5B6-Immunohistochemistry-Paraffin-NBP1-51596-img0008.jpg)
![Flow Cytometry: SMC1 Antibody (5B6) [NBP1-51596] - Analysis of Hela cells using SMC1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/SMC1-Antibody-5B6-Flow-Cytometry-NBP1-51596-img0009.jpg)
![Western Blot: ID2 Antibody (4E12G5) [NBP2-37417] - Analysis using ID2 mAb against human ID2 recombinant protein. (Expected MW is 17.3 kDa).](http://images.novusbio.com/fullsize/ID2-Antibody-4E12G5-Western-Blot-NBP2-37417-img0002.jpg "Western Blot: ID2 Antibody (4E12G5) [NBP2-37417] - Analysis using ID2 mAb against human ID2 recombinant protein. (Expected MW is 17.3 kDa).")
![Immunohistochemistry-Paraffin: ID2 Antibody (4E12G5) [NBP2-37417] - Analysis of rectum cancer tissues using ID2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ID2-Antibody-4E12G5-Immunohistochemistry-NBP2-37417-img0005.jpg)
![Flow Cytometry: ID2 Antibody (4E12G5) [NBP2-37417] - Analysis of SK-N-SH cells using ID2 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/ID2-Antibody-4E12G5-Flow-Cytometry-NBP2-37417-img0003.jpg)
![Immunohistochemistry-Paraffin: ID2 Antibody (4E12G5) [NBP2-37417] - Analysis of breast cancer tissues using ID2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ID2-Antibody-4E12G5-Immunohistochemistry-NBP2-37417-img0004.jpg)
![ELISA: ID2 Antibody (4E12G5) [NBP2-37417] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/ID2-Antibody-4E12G5-ELISA-NBP2-37417-img0001.jpg)
![Western Blot: alpha Adducin Antibody (5D4H1) [NBP2-52409] - Analysis using ADD1 mAb against human ADD1 (AA: 1-193)recombinant protein. (Expected MW is 47.8 kDa)](http://images.novusbio.com/fullsize/alpha-Adducin-Antibody-5D4H1-Western-Blot-NBP2-52409-img0002.jpg "Western Blot: alpha Adducin Antibody (5D4H1) [NBP2-52409] - Analysis using ADD1 mAb against human ADD1 (AA: 1-193)recombinant protein. (Expected MW is 47.8 kDa)")
![Immunohistochemistry-Paraffin: alpha Adducin Antibody (5D4H1) [NBP2-52409] - Analysis of paraffin-embedded cervical cancer tissues using alpha Adducin mAb with DAB staining.](http://images.novusbio.com/fullsize/alpha-Adducin-Antibody-5D4H1-Immunohistochemistry-Paraffin-NBP2-52409-img0006.jpg)
![Western Blot: alpha Adducin Antibody (5D4H1) [NBP2-52409] - Analysis using ADD1 mAb against HEK293 (1) and ADD1 (AA: 1-193)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/alpha-Adducin-Antibody-5D4H1-Western-Blot-NBP2-52409-img0003.jpg)
![Immunohistochemistry-Paraffin: alpha Adducin Antibody (5D4H1) [NBP2-52409] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using 1-193 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/alpha-Adducin-Antibody-5D4H1-Immunohistochemistry-Paraffin-NBP2-52409-img0004.jpg)
![ELISA: alpha Adducin Antibody (5D4H1) [NBP2-52409] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/alpha-Adducin-Antibody-5D4H1-ELISA-NBP2-52409-img0005.jpg)
![Immunohistochemistry-Paraffin: Goat IgG Isotype Control [NB410-28088] - Human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (NB410-28088; left) and Goat Anti-Type III Collagen-UNLB (NBP1-26547; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP DAB, and hematoxylin.](http://images.novusbio.com/fullsize/Goat-IgG-Isotype-Control-Immunohistochemistry-Paraffin-NB410-28088-img0005.jpg "Immunohistochemistry-Paraffin: Goat IgG Isotype Control [NB410-28088] - Human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (NB410-28088; left) and Goat Anti-Type III Collagen-UNLB (NBP1-26547; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP DAB, and hematoxylin.")
![Immunohistochemistry-Paraffin: Goat IgG Isotype Control [NB410-28088] - Human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (NB410-28088; left) and Goat Anti-Type IV Collagen-UNLB (NBP1-26549 right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP DAB, and hematoxylin.](http://images.novusbio.com/fullsize/Goat-IgG-Isotype-Control-Immunohistochemistry-Paraffin-NB410-28088-img0003.jpg)
![Western Blot: Axin-1 Antibody (4E9F1) [NBP2-61695] - Analysis using AXIN1 mAb against human AXIN1 (AA: 546-752) recombinant protein. (Expected MW is 48.7 kDa)](http://images.novusbio.com/fullsize/Axin-1-Antibody-4E9F1-Western-Blot-NBP2-61695-img0004.jpg "Western Blot: Axin-1 Antibody (4E9F1) [NBP2-61695] - Analysis using AXIN1 mAb against human AXIN1 (AA: 546-752) recombinant protein. (Expected MW is 48.7 kDa)")
![Immunocytochemistry/Immunofluorescence: Axin-1 Antibody (4E9F1) [NBP2-61695] - Analysis of Hela cells using AXIN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Axin-1-Antibody-4E9F1-Immunocytochemistry-Immunofluorescence-NBP2-61695-img0003.jpg)
![Flow Cytometry: Axin-1 Antibody (4E9F1) [NBP2-61695] - Analysis of Hela cells using AXIN1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Axin-1-Antibody-4E9F1-Flow-Cytometry-NBP2-61695-img0002.jpg)
![Western Blot: Axin-1 Antibody (4E9F1) [NBP2-61695] - Analysis using AXIN1 mAb against HEK293 (1) and AXIN1 (AA: 546-752)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Axin-1-Antibody-4E9F1-Western-Blot-NBP2-61695-img0005.jpg)
![ELISA: Axin-1 Antibody (4E9F1) [NBP2-61695] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Axin-1-Antibody-4E9F1-ELISA-NBP2-61695-img0001.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75321] - Total cell lysate from human UMUC1. This image was submitted via customer Review. Image from the HRP version of Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-75321-img0004.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75321] - Total cell lysate from human UMUC1. This image was submitted via customer Review. Image from the HRP version of Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).")
![Immunocytochemistry/Immunofluorescence: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166] - Testing of tissue from human cerebral cortex, applying ultra thin sections using a technique called array tomography (antibody: green, DAPI-stained nuclei: blue). Image shows labeling of NPY-positive processes in the neutrophil. Image courtesy of Dr. Nancy O'Rourke, Stanford University.](http://images.novusbio.com/fullsize/Neuropeptide-Y-Antibody-8-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-05166-img0002.jpg "Immunocytochemistry/Immunofluorescence: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166] - Testing of tissue from human cerebral cortex, applying ultra thin sections using a technique called array tomography (antibody: green, DAPI-stained nuclei: blue). Image shows labeling of NPY-positive processes in the neutrophil. Image courtesy of Dr. Nancy O'Rourke, Stanford University.")
![Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166] - Paraffin embedded mouse brain tissue (interneurons in the cortex) diluted 1/1000](http://images.novusbio.com/fullsize/Neuropeptide-Y-Antibody-8-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05166-img0005.jpg)
![Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166] - Paraffin embedded mouse brain tissue (fibres in the hilus of the hippocampus) diluted 1/1000](http://images.novusbio.com/fullsize/Neuropeptide-Y-Antibody-8-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05166-img0003.jpg)
![Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166] - Enlarged section of a paraffin embedded mouse brain tissue (fibres in the hilus of the hippocampus) diluted 1/1000](http://images.novusbio.com/fullsize/Neuropeptide-Y-Antibody-8-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05166-img0004.jpg)
![Immunohistochemistry: Plectin Antibody (7A8) [NB120-11220] - Frozen sections acetone fixed Rat cardiac muscle tissue sections were stained with 1:200 Monoclonal Anti-Plectin antibody produced in Mouse, Clone: 7A8, followed by 1:30 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat.](http://images.novusbio.com/fullsize/Plectin-Antibody-7A8-Immunohistochemistry-NB120-11220-img0001.jpg "Immunohistochemistry: Plectin Antibody (7A8) [NB120-11220] - Frozen sections acetone fixed Rat cardiac muscle tissue sections were stained with 1:200 Monoclonal Anti-Plectin antibody produced in Mouse, Clone: 7A8, followed by 1:30 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat.")
![Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining in human kidney. Strong staining of plasma of nephritis kidney was seen. Dilution: 1:75.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-05122-img0011.jpg "Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining in human kidney. Strong staining of plasma of nephritis kidney was seen. Dilution: 1:75.")
![Western Blot: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Lane 1: purified C3, lane 2: purifed C3 reduced/DTT, lane 3: purified C3a , lane 4: EDTA-plasma. Lane 5: zymosan-activated serum](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Western-Blot-NBP1-05122-img0004.jpg)
![Immunohistochemistry-Paraffin: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining of human kidney. Strong staining of veins of nephritis kidney was seen. Dilution: 1:150.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05122-img0007.jpg)
![Sandwich ELISA Capture: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - The calibration curve of a sandwich assay for Complement C3a using NBP1-05122 as the capture antibody and NBP2-23496 as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Sandwich-ELISA-Capture-NBP1-05122-img0005.jpg)
![Immunohistochemistry: Glucagon Antibody (4) - BSA Free [NBP2-23558] - Endocrine alpha cells from diabetic mice stained with (1:500). Courtesy of Dr. Annette Plesner, University of British Columbia](http://images.novusbio.com/fullsize/GLP1-Antibody-4-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-23558-img0001.jpg "Immunohistochemistry: Glucagon Antibody (4) - BSA Free [NBP2-23558] - Endocrine alpha cells from diabetic mice stained with (1:500). Courtesy of Dr. Annette Plesner, University of British Columbia")
![Glucagon Antibody (4) - BSA Free [NBP2-23558] - BSA Free [NBP2-23558]](http://images.novusbio.com/fullsize/GLP1-Antibody-4-BSA-Free-NBP2-23558-img0002.jpg)