Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2732 | |||
| BSA Free | NB100-2732SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB9895U-100UG | |||
| Alexa Fluor 405 | FAB9895V-100UG | |||
| Alexa Fluor 488 | FAB9895G-100UG | |||
| Alexa Fluor 532 | FAB9895X-100UG | |||
| Alexa Fluor 594 | FAB9895T-100UG | |||
| Alexa Fluor 647 | FAB9895R-100UG | |||
| Alexa Fluor 700 | FAB9895N-100UG | |||
| Alexa Fluor 750 | FAB9895S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB9895-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB100491-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75735 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB104161-SP | |||
| Carrier Free | IC104161V-100UG | |||
| Carrier Free | IC104161U-100UG | |||
| Carrier Free | IC104161T-100UG | |||
| Carrier Free | IC104161S-100UG | |||
| Carrier Free | IC104161R-100UG | |||
| Carrier Free | IC104161N-100UG | |||
| Carrier Free | IC104161G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB11382-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB10940-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB44971-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB11012-SP | |||
| Carrier Free | F0158-100 | |||
| Carrier Free | F0157-100 | |||
| Carrier Free | F0156-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB11323-SP | |||
![Western Blot: CD3 epsilon Antibody (C3e/1931) - Azide and BSA Free [NBP2-79840] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (C3e/1931).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1931-Azide-and-BSA-Free-Western-Blot-NBP2-79840-img0002.jpg "Western Blot: CD3 epsilon Antibody (C3e/1931) - Azide and BSA Free [NBP2-79840] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (C3e/1931).")
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody (C3e/1931) - Azide and BSA Free [NBP2-79840] - Formalin-fixed, paraffin-embedded human Spleen stained with CD3 epsilon Antibody (C3e/1931).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1931-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79840-img0001.jpg)
![Protein Array: CD3 epsilon Antibody (C3e/1931) - Azide and BSA Free [NBP2-79840] - Flow Cytometric Analysis of Jurkat cells. CD3 epsilon Antibody (C3e/1931) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1931-Azide-and-BSA-Free-Protein-Array-NBP2-79840-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys14] Antibody (RM130) - Azide and BSA Free [NBP3-25982] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM130 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-25982_rabbit-histone-h3-mab-rm130-azide-and-bsa-free-2462024132610.png "Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys14] Antibody (RM130) - Azide and BSA Free [NBP3-25982] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM130 (red). Actin filaments have been labeled with fluorescein phalloidin (green).")
![Multiplex Immunoassay: Histone H3 [ac Lys14] Antibody (RM130) - Azide and BSA Free [NBP3-25982] - The binding specificity of RM130 to Histone H3 acetylated at Lysine 14 (K14ac) is not affected by the modification of neighboring amino acids.](http://images.novusbio.com/fullsize/nbp3-25982_rabbit-histone-h3-mab-rm130-azide-and-bsa-free-2462024132621.png)
![Western Blot: Histone H3 [ac Lys14] Antibody (RM130) - Azide and BSA Free [NBP3-25982] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) or, using RM130 at 0.5 ug/mL.](http://images.novusbio.com/fullsize/nbp3-25982_rabbit-histone-h3-mab-rm130-azide-and-bsa-free-2462024133241.png)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys14] Antibody (RM130) - Azide and BSA Free [NBP3-25982] - ChIP performed on HeLa cells using RM130, 5ug. Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp3-25982_rabbit-histone-h3-mab-rm130-azide-and-bsa-free-226202422321239.png)
![Multiplex Immunoassay: Histone H3 [ac Lys14] Antibody (RM130) - Azide and BSA Free [NBP3-25982] - RM130 specifically reacts to Histone H3 acetylated at Lysine 14 (K14ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.](http://images.novusbio.com/fullsize/nbp3-25982_rabbit-histone-h3-mab-rm130-azide-and-bsa-free-24620241245915.png)
![Western Blot: POU2F2 Antibody (Oct2/2137) [NBP2-75734] - Analysis (A) Recombinant Protein (B) Daudi cell lysate POU2F2 Mouse Monoclonal Antibody (OCT2/2137).](http://images.novusbio.com/fullsize/OCT2-Antibody-Oct2-2137-Western-Blot-NBP2-75734-img0004.jpg "Western Blot: POU2F2 Antibody (Oct2/2137) [NBP2-75734] - Analysis (A) Recombinant Protein (B) Daudi cell lysate POU2F2 Mouse Monoclonal Antibody (OCT2/2137).")
![Immunohistochemistry-Paraffin: POU2F2 Antibody (Oct2/2137) [NBP2-75734] - Formalin-fixed, paraffin-embedded human Tonsil stained with POU2F2 Mouse Monoclonal Antibody (OCT2/2137).](http://images.novusbio.com/fullsize/OCT2-Antibody-Oct2-2137-Immunohistochemistry-Paraffin-NBP2-75734-img0002.jpg)
![Western Blot: POU2F2 Antibody (Oct2/2137) [NBP2-75734] - Analysis Ramos cell lysate using POU2F2 Mouse Monoclonal Antibody (OCT2/2137).](http://images.novusbio.com/fullsize/OCT2-Antibody-Oct2-2137-Western-Blot-NBP2-75734-img0003.jpg)
![Immunohistochemistry-Paraffin: POU2F2 Antibody (Oct2/2137) [NBP2-75734] - Formalin-fixed, paraffin-embedded human Lymph Node stained with POU2F2 Mouse Monoclonal Antibody (OCT2/2137).](http://images.novusbio.com/fullsize/OCT2-Antibody-Oct2-2137-Immunohistochemistry-Paraffin-NBP2-75734-img0001.jpg)
![Protein Array: POU2F2 Antibody (Oct2/2137) [NBP2-75734] - Analysis of Protein Array containing more than 19,000 full-length human proteins using POU2F2 Antibody (Oct2/2137). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/OCT2-Antibody-Oct2-2137-Protein-Array-NBP2-75734-img0006.jpg)
