Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NB7187 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB9617-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48282 | |||
| Azide and BSA Free | NBP3-11466 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4749-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2209-SP | |||
| Carrier Free | MAB2209-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF8198-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26497-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34512-0.1mg | |||
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB7186] - An inhibitor of EZH2 reactivates miR-125b-1 in MCF7 breast cancer cells. To determine the dose of GSK126 that reduces H3K27me3 levels, we treated the breast cell lines with different GSK126 concentrations and performed Western blot analysis. Image collected and cropped by CiteAb from the following publication (//springerplus.springeropen.com/articles/10.1186/s40064-016-2475-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NB7186-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB7186] - An inhibitor of EZH2 reactivates miR-125b-1 in MCF7 breast cancer cells. To determine the dose of GSK126 that reduces H3K27me3 levels, we treated the breast cell lines with different GSK126 concentrations and performed Western blot analysis. Image collected and cropped by CiteAb from the following publication (//springerplus.springeropen.com/articles/10.1186/s40064-016-2475-z), licensed under a CC-BY license.")
![Western Blot: Mitochondria Antibody (AE-1) [NBP2-44371] - Western Blot Analysis of HeLa cell lysate using Mitochondria Antibody (AE-1).](http://images.novusbio.com/fullsize/Mitochondria-Antibody-AE-1-Western-Blot-NBP2-44371-img0006.jpg "Western Blot: Mitochondria Antibody (AE-1) [NBP2-44371] - Western Blot Analysis of HeLa cell lysate using Mitochondria Antibody (AE-1).")
![Immunocytochemistry/Immunofluorescence: Mitochondria Antibody (AE-1) [NBP2-44371] - Confocal immunofluorescence image of HeLa cells using Mitochondria Antibody (AE-1) Green (CF488) and Red Dot is used to label the nuclei Red.](http://images.novusbio.com/fullsize/Mitochondria-Antibody-AE-1-Immunocytochemistry-Immunofluorescence-NBP2-44371-img0003.jpg)
![Immunohistochemistry-Paraffin: Mitochondria Antibody (AE-1) [NBP2-44371] - Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Antibody (AE-1).](http://images.novusbio.com/fullsize/Mitochondria-Antibody-AE-1-Immunohistochemistry-Paraffin-NBP2-44371-img0005.jpg)
![Immunohistochemistry-Paraffin: Mitochondria Antibody (AE-1) [NBP2-44371] - Formalin-fixed, paraffin-embedded human Tonsil stained with Mitochondria Antibody (AE-1).](http://images.novusbio.com/fullsize/Mitochondria-Antibody-AE-1-Immunohistochemistry-Paraffin-NBP2-44371-img0004.jpg)
![ELISA p53 [p Ser15] [Biotin]](http://images.novusbio.com/fullsize2/dyc1839-2_human-phospho-p53-s15-duoset-ic-elisa-5-plate-elisa-169202013376.jpg "ELISA p53 [p Ser15] [Biotin]")
![Western Blot: XPF Antibody [NB100-60679] - Detection of Human XPF/ERCC4 on HeLa whole cell lysate using NB100-60679.](http://images.novusbio.com/fullsize/XPF-Antibody-Western-Blot-NB100-60679-img0004.jpg "Western Blot: XPF Antibody [NB100-60679] - Detection of Human XPF/ERCC4 on HeLa whole cell lysate using NB100-60679.")
![Immunohistochemistry-Paraffin: XPF Antibody [NB100-60679] - Human prostate carcinoma. Antibody: Affinity purified rabbit anti- XPF/ERCC4 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/XPF-Antibody-Immunohistochemistry-Paraffin-NB100-60679-img0007.jpg)
![Immunoprecipitation: ATF4 Antibody (9E1) [NBP3-26497] - Immunoprecipitating ATF4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26497 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26497 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-26220241322155.jpg "Immunoprecipitation: ATF4 Antibody (9E1) [NBP3-26497] - Immunoprecipitating ATF4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26497 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26497 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).")
![Immunohistochemistry: ATF4 Antibody (9E1) [NBP3-26497] - Image of ATF4 Antibody (9E1) diluted at 1:160 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-26220241510616.jpg)
![Western Blot: ATF4 Antibody (9E1) [NBP3-26497] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate. All lanes: ATF4 Antibody at 1.6ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 39 KDa Observed band size: 50 KDa](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-282202411472134.jpg)
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody (9E1) [NBP3-26497] - Staining of HepG2 cells with ATF4 Antibody (9E1) at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-28220241312211.jpg)
![Flow Cytometry: ATF4 Antibody (9E1) [NBP3-26497] - Overlay histogram showing Hela cells stained with ATF4 Antibody (9E1) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-282202414312786.jpg)
![Immunohistochemistry-Paraffin: ACTH Antibody (57) - N-terminal [NBP2-32912] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using ACTH monoclonal antibody (57) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/ACTH-Antibody-57-N-terminal-Immunohistochemistry-Paraffin-NBP2-32912-img0002.jpg "Immunohistochemistry-Paraffin: ACTH Antibody (57) - N-terminal [NBP2-32912] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using ACTH monoclonal antibody (57) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: ACTH Antibody (57) - N-terminal [NBP2-32912] - Formalin-fixed, paraffin-embedded human Pituitary Gland stained with ACTH Monoclonal Antibody (57).](http://images.novusbio.com/fullsize/ACTH-Antibody-57-N-terminal-Immunohistochemistry-Paraffin-NBP2-32912-img0001.jpg)
![Immunohistochemistry-Paraffin: ACTH Antibody (57) - N-terminal [NBP2-32912] - Formalin-fixed, paraffin-embedded human pituitary gland stained with ACTH Mouse Monoclonal Antibody (57).](http://images.novusbio.com/fullsize/ACTH-Antibody-57-N-terminal-Immunohistochemistry-Paraffin-NBP2-32912-img0003.jpg)